In extruded feline diets, thiamine degraded at a similar rate when stored at -20°C, compared to room temperature.

Thiamine is an essential dietary nutrient in cats; however, studies on the stability of thiamine in pet food are limited. The objective of this study was to analyze thiamine concentrations in commercial feline extruded diets over time at room and freezing temperatures. Twelve diets were split in half and thiamine concentrations were assessed using fluorometry. One half of each diet was then stored at room temperature (24°C) and the other half was frozen (-20°C). Subsamples were analyzed at 2 other time points at 6-month intervals up to 1 year. Data were assessed using a mixed procedure (2-factor factorial model with factors time and treatment). Based on F-tests, thiamine concentrations decreased over time (P = 0.001), with no treatment*time interaction (P = 0.9534). In conclusion, regardless of treatment, thiamine degraded at a similar rate over time.

Dans les aliments extrudés pour félins, la thiamine s'est dégradée à un taux similaire lorsqu'elle est conservée à −20 °C par rapport à la température ambiante. La thiamine est un nutriment alimentaire essentiel chez les chats; cependant, les études sur la stabilité de la thiamine dans les aliments pour animaux de compagnie sont limitées. L'objectif de la présente étude était d'analyser les concentrations de thiamine dans les aliments extrudés commerciaux pour félins au fil du temps à des températures ambiantes et de congélation. Douze aliments ont été divisés en deux et les concentrations de thiamine ont été évaluées par fluorométrie. La moitié de chaque aliment a ensuite été conservée à température ambiante (24 °C) et l'autre moitié a été congelée (−20 °C). Les sous-échantillons ont été analysés à deux moments supplémentaires à des intervalles de 6 mois jusqu'à 1 an. Les données ont été évaluées à l'aide d'une procédure mixte (modèle factoriel à deux facteurs avec les facteurs temps et traitement). Les tests F globaux ont montré une diminution de la concentration de thiamine au fil du temps (P = 0,001) et aucune interaction entre le traitement et le temps (P = 0,9534). En conclusion, quel que soit le traitement, la thiamine s'est dégradée à un rythme similaire dans le temps.(Traduit par Dr Serge Messier).

Quantification of vitamin D3 in commercial dog foods and comparison with Association of American Feed Control Officials recommendations and manufacturer-reported concentrations.

OBJECTIVE To quantify vitamin D3 (VitD3) concentrations in commercial dog foods and compare those concentrations with Association of American Feed Control Officials (AAFCO) recommendations and manufacturer-reported concentrations. DESIGN Cross-sectional study. SAMPLE 82 commercial dog foods. PROCEDURES Samples of commercially available dog foods were obtained from owners of healthy dogs in the Guelph, ON, Canada, area and owners of dogs that were patients at the Ontario Veterinary College Health Sciences Centre's Mona Campbell Centre for Animal Cancer. For each food, the VitD3 concentration was determined by high-performance liquid chromatography-tandem mass spectrometry, and adherence to AAFCO and National Research Council recommendations was assessed. Analyzed VitD3 concentrations were compared with manufacturer-reported VitD3 concentrations and between wet and dry foods, among AAFCO nutritional adequacy statements (nutrient profiles vs feeding trials and adult maintenance vs all life stages), between foods sold only by veterinarians and those sold over the counter, and between small and large manufacturers. RESULTS The analyzed VitD3 concentration was below both AAFCO and National Research Council recommendations for one sample and below the assay detection limit for another. Analyzed VitD3 concentrations did not differ significantly from manufacturer-reported VitD3 concentrations or between wet and dry foods, among foods with different AAFCO nutritional adequacy statements, between foods sold only by veterinarians and those sold over the counter, or between foods produced by small and large manufacturers. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that manufacturer-reported VitD3 concentrations were accurate and that dog owners can be confident that VitD3 intake is adequate for AAFCO-compliant commercial dog foods.