RESUMO
Synthesis of an artificial gene encoding the signal peptide of the Yersinia pestis capsule antigen (Caf1) was accomplished. A set of plasmids coding for hybrid proteins in which a modified sequence of the Caf1 signal peptide is connected to the amino acid sequence of the mature granulocyte-macrophage colony stimulating factor (GM-CSF) were constructed. Topography of the cleavage site of signal proteases was studied. The presence of an arginine residue within the N-terminal part of the mature human GM-CSF was shown to hinder the proper processing and translocation of the precursor through periplasmic membrane. A number of E. coli strains secreting biologically active mutants of human GM-CSF were obtained.
Assuntos
Fator Estimulador de Colônias de Granulócitos e Macrófagos/metabolismo , Sinais Direcionadores de Proteínas/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Escherichia coli/genética , Escherichia coli/metabolismo , Fator Estimulador de Colônias de Granulócitos e Macrófagos/genética , Humanos , Dados de Sequência Molecular , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismoAssuntos
Acidentes , Exposição Ambiental/efeitos adversos , Militares , Reatores Nucleares , Centrais Elétricas , Poluentes Radioativos/efeitos adversos , Glândula Tireoide/diagnóstico por imagem , Glândula Tireoide/efeitos da radiação , Feminino , Humanos , Hiperplasia/diagnóstico por imagem , Masculino , República de Belarus , Federação Russa , Glândula Tireoide/patologia , Ucrânia , UltrassonografiaRESUMO
The authors studied the prevalence, clinical state and socio-labour adaptation of schizophrenic patients studying at multiprofile institutes, within a large student campus. Among the students of technical faculties schizophrenic patients were registered 7 times less than among the students of the physico-mathematical faculty and 4 times less than among the students of humanitarian faculties. Half of the students with schizophrenia successfully enough continue to study and graduate from the institutes. The degree of socio-labour adaptation of the sick students did not depend upon the profile of the faculty, but was due mainly to the progressiveness of the schizophrenic process and the rehabilitative programmes. The authors recommend a perfection of mental health services to the students, a wider inclusion of clinical indications and individual approaches to the admittance and possible studies of schizophrenic patients, at establishments of higher education.
