RESUMO
Changes in the fatty acid composition and ultrastructure of Pseudomonas aeruginosa 1C strain--a destructor of alkyl sulphates under the effect of the stress evoked by sodium dodecyl-sulphate have been studied. It has been established that the "detergent" stress changes the ratio of the cell fatty acids (FA) towards the increase of their nonsaturation. Ultrastructural changes in the cells are revealed earlier than biochemical ones. Several stages in stress development have been distinguished. The escape from the stress state is performed both at the population level and at the level of individual cells, highly-resistant to dodecyl sulphate.
Assuntos
Ácidos Graxos/análise , Lipídeos de Membrana/metabolismo , Pseudomonas aeruginosa/efeitos dos fármacos , Dodecilsulfato de Sódio/farmacologia , Tensoativos/farmacologia , Detergentes/metabolismo , Lipídeos de Membrana/química , Microscopia Eletrônica , Pseudomonas aeruginosa/metabolismo , Pseudomonas aeruginosa/ultraestruturaAssuntos
Pseudomonas aeruginosa/fisiologia , Ésteres do Ácido Sulfúrico/metabolismo , Ácidos Sulfúricos/metabolismo , Proteínas da Membrana Bacteriana Externa/análise , Movimento Celular/efeitos dos fármacos , Fenômenos Químicos , Físico-Química , Eletroforese , Variação Genética , Concentração de Íons de Hidrogênio , Cinética , Lipopolissacarídeos/análise , Potenciais da Membrana/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Dodecilsulfato de Sódio/farmacologia , Propriedades de SuperfícieAssuntos
Variação Genética , Pseudomonas aeruginosa/enzimologia , Ésteres do Ácido Sulfúrico/metabolismo , Ácidos Sulfúricos/metabolismo , Antibacterianos/farmacologia , Biodegradação Ambiental , Resistência Microbiana a Medicamentos , Variação Genética/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Dodecilsulfato de Sódio/metabolismo , Dodecilsulfato de Sódio/farmacologiaRESUMO
The fatty acid composition of Pseudomonas aeruginosa 1C destroying anionic surfactant alkyl sulfates was studied after its cultivation under different conditions which caused different resistance of the cells against sodium dodecyl sulfate (SDS). The content of monounsaturated fatty acids (in particular, octadecenoic acid) increased while the content of cyclopropane fatty acids decreased in cells resistant against SDS.
Assuntos
Ácidos Graxos/metabolismo , Pseudomonas aeruginosa/metabolismo , Dodecilsulfato de Sódio/farmacologia , Resistência Microbiana a Medicamentos , Pseudomonas aeruginosa/efeitos dos fármacosRESUMO
The products of Na dodecyl sulphate destruction by the three bacterial cultures--Pseudomonas aeruginosa, Flavobacterium devorans, Achromobacter guttatus--were examined. The cultures were shown to decompose Na dodecyl sulphate in a similar way. The primary mechanism of destruction was found to be hydrolysis of the sulpho-ester bond in the molecule, leading to the separation of sulphate-ion and formation of dodecanol. Products of bacterial destruction of alkyl sulphates did not show foam forming capacity.
Assuntos
Alcaligenes/metabolismo , Flavobacterium/metabolismo , Pseudomonas aeruginosa/metabolismo , Dodecilsulfato de Sódio/metabolismo , Hidrólise , Especificidade da EspécieRESUMO
The following bacteria assimilating alkyl sulphates as a sole source of carbon and energy were isolated from various substrates: Pseudomonas, Achromobacter, Flavobacterium, Citrobacter, Enterobacter. The bacteria decomposed sodium dodecyl sulphate at a high rate, and some of them, industrial preparations of alkyl sulphates. The ability to assimilate alkyl sulphates was tested in 257 collection cultures belonging to different taxonomic groups. Alkyl sulphates were found to be decomposed by heterotrophous gram-negative rod-like bacteria belonging to different families and genera. The frequency of bacteria destroying alkyl sulphates at a high rate was found most often in the Pseudomonas genus.
