Mathematical modeling based on RT-qPCR analysis of SARS-CoV-2 in wastewater as a tool for epidemiology.

Coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerges to scientific research and monitoring of wastewaters to predict the spread of the virus in the community. Our study investigated the COVID-19 disease in Bratislava, based on wastewater monitoring from September 2020 until March 2021. Samples were analyzed from two wastewater treatment plants of the city with reaching 0.6 million monitored inhabitants. Obtained results from the wastewater analysis suggest significant statistical dependence. High correlations between the number of viral particles in wastewater and the number of reported positive nasopharyngeal RT-qPCR tests of infected individuals with a time lag of 2 weeks/12 days (R2 = 83.78%/R2 = 52.65%) as well as with a reported number of death cases with a time lag of 4 weeks/27 days (R2 = 83.21%/R2 = 61.89%) was observed. The obtained results and subsequent mathematical modeling will serve in the future as an early warning system for the occurrence of a local site of infection and, at the same time, predict the load on the health system up to two weeks in advance.

Environmental risk of nanomaterials and nanoparticles and EPR technique as an effective tool to study them-a review.

Nanotechnologies and different types of nanomaterials belong in present day to intensively studied materials due to their unique properties and diverse potential applications in, e.g., electronics, medicine, or display technologies. Together with the investigation of their desired beneficial properties, a need to investigate and evaluate their influence on the environment and possible harmful effects towards living organisms is growing. This review summarizes possible toxic effects of nanomaterials on environment and living organisms, focusing on the possible bioaccumulation in organisms, toxicity, and its mechanisms. The main goal of this review is to refer to potential environmental risks rising from the use of nanomaterials and the necessity to deal with the possible toxic effects considering the growing interest in the wide-scale utilization of these materials. Electron paramagnetic resonance spectroscopy as the only analytical technique capable of detecting radical species enables detection, quantification, and monitoring of the generation of short-lived radicals often coupled with toxic effects of nanomaterials, which makes it an important method in the process of nanotoxicity mechanism determination.