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1.
Sci Rep ; 12(1): 722, 2022 01 14.
Artigo em Inglês | MEDLINE | ID: mdl-35031635

RESUMO

Following its association with dyslexia in multiple genetic studies, the KIAA0319 gene has been extensively investigated in different animal models but its function in neurodevelopment remains poorly understood. We developed the first human cellular knockout model for KIAA0319 in RPE1 retinal pigment epithelia cells via CRISPR-Cas9n to investigate its role in processes suggested but not confirmed in previous studies, including cilia formation and cell migration. We observed in the KIAA0319 knockout increased cilia length and accelerated cell migration. Using Elastic Resonator Interference Stress Microscopy (ERISM), we detected an increase in cellular force for the knockout cells that was restored by a rescue experiment. Combining ERISM and immunostaining we show that RPE1 cells exert highly dynamic, piconewton vertical pushing forces through actin-rich protrusions that are surrounded by vinculin-rich pulling sites. This protein arrangement and force pattern has previously been associated to podosomes in other cells. KIAA0319 depletion reduces the fraction of cells forming these actin-rich protrusions. Our results suggest an involvement of KIAA0319 in cilia biology and cell-substrate force regulation.


Assuntos
Comunicação Celular/genética , Comunicação Celular/fisiologia , Movimento Celular/genética , Movimento Celular/fisiologia , Cílios/genética , Cílios/fisiologia , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/fisiologia , Epitélio Pigmentado da Retina/citologia , Actinas/metabolismo , Sistemas CRISPR-Cas , Linhagem Celular , Humanos , Microscopia de Interferência , Modelos Genéticos , Podossomos/fisiologia , Epitélio Pigmentado da Retina/metabolismo , Vinculina/metabolismo
2.
Nat Commun ; 12(1): 3552, 2021 06 11.
Artigo em Inglês | MEDLINE | ID: mdl-34117241

RESUMO

Important dynamic processes in mechanobiology remain elusive due to a lack of tools to image the small cellular forces at play with sufficient speed and throughput. Here, we introduce a fast, interference-based force imaging method that uses the illumination of an elastic deformable microcavity with two rapidly alternating wavelengths to map forces. We show real-time acquisition and processing of data, obtain images of mechanical activity while scanning across a cell culture, and investigate sub-second fluctuations of the piconewton forces exerted by macrophage podosomes. We also demonstrate force imaging of beating neonatal cardiomyocytes at 100 fps which reveals mechanical aspects of spontaneous oscillatory contraction waves in between the main contraction cycles. These examples illustrate the wider potential of our technique for monitoring cellular forces with high throughput and excellent temporal resolution.


Assuntos
Diagnóstico por Imagem/métodos , Mecanotransdução Celular/fisiologia , Microscopia de Interferência/métodos , Animais , Adesão Celular , Fibroblastos/citologia , Humanos , Macrófagos/citologia , Camundongos , Modelos Teóricos , Células NIH 3T3 , Podossomos/metabolismo
3.
Int J Mol Sci ; 22(8)2021 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-33921304

RESUMO

Local basement membrane (BM) disruption marks the initial step of breast cancer invasion. The activation mechanisms of force-driven BM-weakening remain elusive. We studied the mechanical response of MCF10A-derived human breast cell acini with BMs of tuneable maturation to physical and soluble tumour-like extracellular matrix (ECM) cues. Traction force microscopy (TFM) and elastic resonator interference stress microscopy (ERISM) were used to quantify pro-invasive BM stress and protrusive forces. Substrate stiffening and mechanically impaired BM scaffolds induced the invasive transition of benign acini synergistically. Robust BM scaffolds attenuated this invasive response. Additional oncogenic EGFR activation compromised the BMs' barrier function, fuelling invasion speed and incidence. Mechanistically, EGFR-PI3-Kinase downstream signalling modulated both MMP- and force-driven BM-weakening processes. We show that breast acini form non-proteolytic and BM-piercing filopodia for continuous matrix mechanosensation, which significantly push and pull on the BM and ECM under pro-invasive conditions. Invasion-triggered acini further shear and compress their BM by contractility-based stresses that were significantly increased (3.7-fold) compared to non-invasive conditions. Overall, the highest amplitudes of protrusive and contractile forces accompanied the highest invasiveness. This work provides a mechanistic concept for tumour ECM-induced mechanically misbalanced breast glands fuelling force-driven BM disruption. Finally, this could facilitate early cell dissemination from pre-invasive lesions to metastasize eventually.


Assuntos
Mama/metabolismo , Fator de Crescimento Epidérmico/genética , Neoplasias/genética , Células Acinares/metabolismo , Células Acinares/patologia , Membrana Basal/metabolismo , Membrana Basal/patologia , Mama/patologia , Linhagem Celular Tumoral , Receptores ErbB/genética , Matriz Extracelular/genética , Matriz Extracelular/patologia , Feminino , Humanos , Glândulas Mamárias Humanas/patologia , Fenômenos Mecânicos , Invasividade Neoplásica/genética , Neoplasias/patologia , Pseudópodes/genética , Pseudópodes/patologia
4.
Front Cell Neurosci ; 14: 552213, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33088261

RESUMO

Willin/FRMD6 is part of a family of proteins with a 4.1 ezrin-radixin-moesin (FERM) domain. It has been identified as an upstream activator of the Hippo pathway and, when aberrant in its expression, is associated with human diseases and disorders. Even though Willin/FRMD6 was originally discovered in the rat sciatic nerve, most studies have focused on its functional roles in cells outside of the nervous system, where Willin/FRMD6 is involved in the formation of apical junctional cell-cell complexes and in regulating cell migration. Here, we investigate the biochemical and biophysical role of Willin/FRMD6 in neuronal cells, employing the commonly used SH-SY5Y neuronal model cell system and combining biochemical measurements with Elastic Resonator Interference Stress Micropscopy (ERISM). We present the first direct evidence that Willin/FRMD6 expression influences both the cell mechanical phenotype and neuronal differentiation. By investigating cells with increased and decreased Willin/FRMD6 expression levels, we show that Willin/FRMD6 not only affects proliferation and migration capacity of cells but also leads to changes in cell morphology and an enhanced formation of neurite-like membrane extensions. These changes were accompanied by alterations of biophysical parameters such as cell force, the organization of actin stress fibers and the formation of focal adhesions. At the biochemical level, changes in Willin/FRMD6 expression inversely affected the activity of the extracellular signal-regulated kinases (ERK) pathway and downstream transcriptional factor NeuroD1, which seems to prime SH-SY5Y cells for retinoic acid (RA)-induced neuronal differentiation.

5.
Nat Mater ; 19(9): 1019-1025, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32451510

RESUMO

Cortical stiffness is an important cellular property that changes during migration, adhesion and growth. Previous atomic force microscopy (AFM) indentation measurements of cells cultured on deformable substrates have suggested that cells adapt their stiffness to that of their surroundings. Here we show that the force applied by AFM to a cell results in a significant deformation of the underlying substrate if this substrate is softer than the cell. This 'soft substrate effect' leads to an underestimation of a cell's elastic modulus when analysing data using a standard Hertz model, as confirmed by finite element modelling and AFM measurements of calibrated polyacrylamide beads, microglial cells and fibroblasts. To account for this substrate deformation, we developed a 'composite cell-substrate model'. Correcting for the substrate indentation revealed that cortical cell stiffness is largely independent of substrate mechanics, which has major implications for our interpretation of many physiological and pathological processes.


Assuntos
Córtex Cerebral/citologia , Diferenciação Celular , Módulo de Elasticidade , Microscopia de Força Atômica/métodos , Especificidade por Substrato
6.
Sci Adv ; 4(6): eaap8030, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29963620

RESUMO

In the healthy kidney, specialized cells called podocytes form a sophisticated blood filtration apparatus that allows excretion of wastes and excess fluid from the blood while preventing loss of proteins such as albumin. To operate effectively, this filter is under substantial hydrostatic mechanical pressure. Given their function, it is expected that the ability to apply mechanical force is crucial to the survival of podocytes. However, to date, podocyte mechanobiology remains poorly understood, largely because of a lack of experimental data on the forces involved. We perform quantitative, continuous, nondisruptive, and high-resolution measurements of the forces exerted by differentiated podocytes in real time using a recently introduced functional imaging modality for continuous force mapping. Using an accepted model for podocyte injury, we find that injured podocytes experience near-complete loss of cellular force transmission but that this loss of force is reversible under certain conditions. The observed changes in force correlate with F-actin rearrangement and reduced expression of podocyte-specific proteins. By introducing robust and high-throughput mechanical phenotyping and by demonstrating the significance of mechanical forces in podocyte injury, this research paves the way to a new level of understanding of the kidney. In addition, in an advance over established force mapping techniques, we integrate cellular force measurements with immunofluorescence and perform continuous long-term force measurements of a cell population. Hence, our approach has general applicability to a wide range of biomedical questions involving mechanical forces.


Assuntos
Biomarcadores , Fenômenos Biomecânicos , Mecanotransdução Celular , Podócitos/metabolismo , Animais , Diferenciação Celular , Citoesqueleto/metabolismo , Imunofluorescência , Humanos , Camundongos , Fenótipo , Podócitos/citologia , Estresse Fisiológico
7.
Nat Commun ; 9(1): 1525, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29717120

RESUMO

Organic semiconductors enable the fabrication of a range of lightweight and mechanically flexible optoelectronic devices. Most organic semiconductor lasers, however, have remained rigid until now, predominantly due to the need for a support substrate. Here, we use a simple fabrication process to make membrane-based, substrate-less and extremely thin (<500 nm) organic distributed feedback lasers that offer ultralow-weight (m/A<0.5 gm-2) and excellent mechanical flexibility. We show operation of the lasers as free-standing membranes and transfer them onto other substrates, e.g. a banknote, where the unique lasing spectrum is readily read out and used as security feature. The pump thresholds and emission intensity of our membrane lasers are well within the permissible exposures for ocular safety and we demonstrate integration on contact lenses as wearable security tags.

8.
Nat Cell Biol ; 19(7): 864-872, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28628084

RESUMO

Cellular forces are crucial for many biological processes but current methods to image them have limitations with respect to data analysis, resolution and throughput. Here, we present a robust approach to measure mechanical cell-substrate interactions in diverse biological systems by interferometrically detecting deformations of an elastic micro-cavity. Elastic resonator interference stress microscopy (ERISM) yields stress maps with exceptional precision and large dynamic range (2 nm displacement resolution over a >1 µm range, translating into 1 pN force sensitivity). This enables investigation of minute vertical stresses (<1 Pa) involved in podosome protrusion, protein-specific cell-substrate interaction and amoeboid migration through spatial confinement in real time. ERISM requires no zero-force reference and avoids phototoxic effects, which facilitates force monitoring over multiple days and at high frame rates and eliminates the need to detach cells after measurements. This allows observation of slow processes such as differentiation and further investigation of cells, for example, by immunostaining.


Assuntos
Movimento Celular , Dictyostelium/fisiologia , Fibroblastos/fisiologia , Macrófagos/fisiologia , Microscopia de Interferência/métodos , Podossomos/fisiologia , Linfócitos T/fisiologia , Células 3T3 , Animais , Fenômenos Biomecânicos , Adesão Celular , Dictyostelium/metabolismo , Elasticidade , Matriz Extracelular/metabolismo , Fibroblastos/metabolismo , Humanos , Macrófagos/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Transgênicos , Microscopia de Vídeo , Podossomos/metabolismo , Estresse Mecânico , Linfócitos T/metabolismo , Fatores de Tempo , Imagem com Lapso de Tempo
9.
Sci Adv ; 2(8): e1600666, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27551686

RESUMO

Under adequate conditions, cavity polaritons form a macroscopic coherent quantum state, known as polariton condensate. Compared to Wannier-Mott excitons in inorganic semiconductors, the localized Frenkel excitons in organic emitter materials show weaker interaction with each other but stronger coupling to light, which recently enabled the first realization of a polariton condensate at room temperature. However, this required ultrafast optical pumping, which limits the applications of organic polariton condensates. We demonstrate room temperature polariton condensates of cavity polaritons in simple laminated microcavities filled with biologically produced enhanced green fluorescent protein (eGFP). The unique molecular structure of eGFP prevents exciton annihilation even at high excitation densities, thus facilitating polariton condensation under conventional nanosecond pumping. Condensation is clearly evidenced by a distinct threshold, an interaction-induced blueshift of the condensate, long-range coherence, and the presence of a second threshold at higher excitation density that is associated with the onset of photon lasing.


Assuntos
Lasers , Proteínas Luminescentes/química , Proteínas de Fluorescência Verde/química , Fótons , Análise Espectral
10.
Nano Lett ; 15(8): 5647-52, 2015 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-26186167

RESUMO

We report on a laser that is fully embedded within a single live cell. By harnessing natural endocytosis of the cell, we introduce a fluorescent whispering gallery mode (WGM) microresonator into the cell cytoplasm. On pumping with nanojoule light pulses, green laser emission is generated inside the cells. Our approach can be applied to different cell types, and cells with microresonators remain viable for weeks under standard conditions. The characteristics of the lasing spectrum provide each cell with a barcode-type label which enables uniquely identifying and tracking individual migrating cells. Self-sustained lasing from cells paves the way to new forms of cell tracking, intracellular sensing, and adaptive imaging.


Assuntos
Rastreamento de Células/métodos , Animais , Movimento Celular , Sobrevivência Celular , Células Cultivadas , Células HEK293 , Humanos , Lasers , Macrófagos/citologia , Camundongos , Microglia/citologia , Células NIH 3T3
13.
Org Lett ; 12(16): 3666-9, 2010 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-20704411

RESUMO

A series of near-infrared absorbing merocyanine dyes bearing the strong electron-accepting 2-oxo-5-dicyanomethylene-pyrrolidine unit was synthesized and applied in combination with PC(61)BM and PC(71)BM in solution-processed photoactive layers of bulk heterojunction solar cells, exhibiting a remarkable performance range with power conversion efficiencies from 0.01% to 1.00%.

14.
Adv Mater ; 22(37): 4193-7, 2010 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-20512818

RESUMO

Identically configured bulk heterojunction organic solar cells based on merocyanine dye donor and fullerene acceptor compounds (see figure) are manufactured either from solution or by vacuum deposition, to enable a direct comparison. Whereas the former approach is more suitable for screening purposes, the latter approach affords higher short-circuit current density and power conversion efficiency.


Assuntos
Corantes Fluorescentes/química , Pirimidinonas/química , Energia Solar , Compostos Bicíclicos Heterocíclicos com Pontes/química , Fulerenos/química , Polímeros/química , Poliestirenos/química , Teoria Quântica
15.
Chem Commun (Camb) ; (48): 6489-91, 2008 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-19057755

RESUMO

Traditional low-molecular weight colorants that are widely applied in textile coloration, for printing purposes and nonlinear optics, now afford bulk heterojunction solar cells in combination with soluble C(60) fullerene derivative PCBM with power conversion efficiencies up to 1.7% under standard solar radiation.

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