RESUMO
In 7 children aged 18 months to 7 years isolated from a group of 128 children with infectious mononucleosis the cytomegalovirus infection was found. Infection was diagnosed by determination of antibodies against immediate early and late CMV antigen by means of the ELISA test. Besides that, antibodies were determined against the capsid antigen and early antigen of EB virus by the method of indirect immunofluorescence. In four children only cytomegalovirus infection was found and three had a mixed infection with both viruses and the diagnosis in these cases was: infectious mononucleosis (due to EBV) with coexistent or following CMV infection. In the sera of two children with cytomegalovirus mononucleosis changes were observed in the antibodies against EBV which is explained as a result of interactions between CMV and EBV in the organism of the host.
Assuntos
Proteínas do Capsídeo , Infecções por Citomegalovirus/diagnóstico , Mononucleose Infecciosa/etiologia , Adolescente , Anticorpos Antivirais/análise , Antígenos Virais/imunologia , Criança , Pré-Escolar , Citomegalovirus/imunologia , Infecções por Citomegalovirus/complicações , Ensaio de Imunoadsorção Enzimática , Herpesvirus Humano 4/imunologia , Humanos , Imunoglobulina G/análise , Imunoglobulina M/análise , Lactente , Mononucleose Infecciosa/complicações , Mononucleose Infecciosa/diagnósticoAssuntos
Anticorpos Antivirais/isolamento & purificação , Antígenos Virais/imunologia , Capsídeo/imunologia , Herpesvirus Humano 4/imunologia , Mononucleose Infecciosa/diagnóstico , Proteínas Virais/imunologia , Adolescente , Criança , Pré-Escolar , Imunofluorescência , Humanos , Lactente , Mononucleose Infecciosa/microbiologiaRESUMO
Cytophotometric determinations of DNA were carried out with a pulse cytophotometer in peripheral blood leucocytes in 25 patients with infectious mononucleosis and 30 patients with other infectious diseases. DNA cytograms of patients with mononucleosis showed presence of a high percent of cells in phase S and G2+M of the cell cycle. This is an evidence of active proliferation of these cells persisting for about 6--8 weeks. The authors believe that pulse cytophotometry may be used for detection and investigation of states with lymphocytes stimulation in vivo, e.g. in certain infectious diseases, during immunotherapy of neoplasms, rejection reaction etc.
