RESUMO
Using tryptophan fluorescence quenching by iodide and acrylamide the effect of electrons with the energy 5 Mev on the structure of microsomal membrane proteins has been studied. Conformation of microsomal proteins were found to change upon irradiation.
Assuntos
Elétrons , Proteínas de Membrana/metabolismo , Microssomos Hepáticos/efeitos da radiação , Acrilamida/química , Animais , Fluorescência , Iodetos/química , Proteínas de Membrana/química , Conformação Proteica , Coelhos , Triptofano/químicaRESUMO
The effect of triton X-100 and glutaraldehyde on the changes in Ca(2+)- and Mg(2+)-ATPase activity of erythrocyte plasma membrane under irradiation of rat erythrocytes has been studied. It was found that irradiation of erythrocyte ghosts in a dose of 1000 Gy leads to the inhibition of the both enzymes. Triton X-100 enhanced the radiation effect, while glutaraldehyde decreased Ca(2+)-ATPase sensitivity to the action of radiation. The mechanisms of the effects observed are discussed.
Assuntos
ATPase de Ca(2+) e Mg(2+)/efeitos dos fármacos , ATPases Transportadoras de Cálcio/efeitos dos fármacos , Membrana Eritrocítica/efeitos dos fármacos , Glutaral/farmacologia , Octoxinol/farmacologia , Animais , ATPase de Ca(2+) e Mg(2+)/sangue , ATPases Transportadoras de Cálcio/sangue , Detergentes , Membrana Eritrocítica/enzimologia , Membrana Eritrocítica/efeitos da radiação , Membrana Eritrocítica/ultraestrutura , RatosRESUMO
Influence of the 5 MeV electron beam on the structural dynamic organization of the protein phase of erythrocyte shadows has been studied by suppression of the tryptophane fluorescence of proteins by acrylamide. It has been observed that the irradiation leads to conformation changes and increase of the structural rigidity of the protein molecules.
Assuntos
Membrana Eritrocítica/efeitos da radiação , Proteínas de Membrana/química , Acrilamida , Acrilamidas/química , Animais , Bovinos , Elétrons , Conformação ProteicaRESUMO
The effect of electrons with energy 5 MeV on the functional state of rabbit liver microsome oxidoreductases has been investigated. Decrease in enzyme activity upon irradiation with a dose of 10 kGy was found. The possible mechanisms of the effects observed are discussed.
Assuntos
Microssomos Hepáticos/enzimologia , Oxirredutases/antagonistas & inibidores , Animais , Relação Dose-Resposta à Radiação , Técnicas In Vitro , Peroxidação de Lipídeos/efeitos da radiação , Malondialdeído/metabolismo , Microssomos Hepáticos/metabolismo , Microssomos Hepáticos/efeitos da radiação , Coelhos , Tolerância a RadiaçãoRESUMO
The effect of electrons with the energy of 5 MeV on the erythrocyte membrane structure was investigated using a fluorescent probe (4-dimethylaminostiryl)-1-methylpyridinium (DSM). Analysis of a competitive binding of DSM and ribonuclease with the erythrocyte ghosts has shown that irradiation causes an increase in the constant of protein association with membranes. It is suggested that a negative surface change increase with irradiation.
Assuntos
Membrana Eritrocítica/efeitos da radiação , Corantes Fluorescentes , Compostos de Piridínio/sangue , Ribonucleases/sangue , Animais , Ligação Competitiva , Membrana Eritrocítica/metabolismo , Membrana Eritrocítica/ultraestrutura , RatosRESUMO
Electrons with energy 5 MeV have been studied for their effect on the electrokinetic properties of erythrocytes. The electrophoretic mobility of the cells was shown to decrease under the effect of radiation. It is supposed that the effect observed is determined by the structural changes of the membrane proteins.
Assuntos
Elétrons , Eritrócitos/efeitos da radiação , Animais , Eletroforese , RatosRESUMO
Using fluorescent probe 4-(n-dimethyl-aminostyryl)-1-methyl-pyridine (DSM) the effect of electrons with the energy 5 MeV on the structure of erythrocyte membrane has been investigated. It was found that in the irradiated erythrocyte ghosts the number of DSM binding sites increases. Irradiation was suggested to increase the negative surface charge of the erythrocyte membrane.
Assuntos
Membrana Eritrocítica/efeitos da radiação , Animais , Sítios de Ligação , Elétrons , Membrana Eritrocítica/metabolismo , Corantes Fluorescentes/metabolismo , Compostos de Piridínio/metabolismo , RatosRESUMO
Effect of electrons with the energy 5MeV on the erythrocyte ghosts structure has been investigated using fluorescent probes 1.8-ANS and DSP-12. The number of ANS binding sites was shown to decrease as affected by irradiation. It was found that radiation caused reduction of the erythrocyte membrane surface area.
