RESUMO
In recent years, much attention has been paid to the development of tissue engineering and regenerative medicine, especially when stem cells of various sources are concerned. In addition to the interest in mesenchymal stem cells isolated from bone marrow, recently more consideration has been given to stem cells isolated from adipose tissue (AdMSCs), due to their less invasive method of collection as well as their ease of isolation and culture. However, the development of regenerative medicine requires both the application of biocompatible material and the stem cells to accelerate the regeneration. In this study, we investigated the morphology, proliferation rate index (PRi), and population doubling time factor of adipose-derived mesenchymal stem cells cultured on non-aqueous sol-gel-derived SiO2, TiO2, and SiO2/TiO2 oxide coatings. The results indicated an increase in PRi of AdMSCs when cultured on to titanium dioxide, suggesting its high attractiveness for AdMSCs. In addition, the proper morphology and the shortest doubling time of AdMSCs were observed when cultured on titanium dioxide coating.
Assuntos
Tecido Adiposo/metabolismo , Proliferação de Células , Materiais Revestidos Biocompatíveis/química , Células-Tronco Mesenquimais/metabolismo , Dióxido de Silício/química , Titânio/química , Tecido Adiposo/citologia , Animais , Células Cultivadas , Teste de Materiais/métodos , Células-Tronco Mesenquimais/citologia , Transição de Fase , Ratos , Ratos WistarRESUMO
In this study, metal-based biomaterials were functionalized with ascorbic acid (LAA). Two types of substrates were used: austenitic steel 316L and titanium Ti6Al4V. Coatings were prepared with the sol-gel method and applied on metal surfaces using the dip-coating technique. Ascorbic acid was delivered with SiO2-coating at concentrations of 0.1 and 0.4 M. The morphology of the surfaces and coatings was determined using scanning electron microscope (SEM), whereas their elemental composition by SEM-EDX. Immobilization of ascorbic acid in the coatings was confirmed with Raman spectroscopy. The biocompatibility of the materials obtained was tested in vitro using both bone marrow- and adipose-derived mesenchymal stem cells (BMMSC and ADMSC, respectively). Proliferation rate and morphology of cells cultured in the presence of designed biomaterials were monitored after 24, 48, 120 and 168 h of propagation. The results obtained indicated that silica coatings doped with 0.4 M LAA had a positive effect on the proliferation rate of investigated cells, and in some cases on the growth pattern of culture.
Assuntos
Materiais Biocompatíveis/química , Teste de Materiais/métodos , Células-Tronco Mesenquimais/citologia , Tecido Adiposo/citologia , Ligas , Animais , Ácido Ascórbico/química , Células da Medula Óssea/citologia , Proliferação de Células , Células Cultivadas , Materiais Revestidos Biocompatíveis/química , Microscopia Eletrônica de Varredura , Ratos , Medicina Regenerativa , Dióxido de Silício/química , Espectrometria por Raios X , Análise Espectral Raman , Aço Inoxidável/química , Propriedades de Superfície , Titânio/químicaRESUMO
Pure and calcium-doped silica and titanium dioxide thin films were prepared by the sol-gel method. Two different metallic substrates, i.e. stainless steel (316L) and titanium alloy (Ti6Al4V), were used for thin film deposition. Physicochemical properties and roughness of the thin films derived were investigated using the Raman spectroscopy, X-ray diffraction analysis, scanning electron microscopy and Taylor-Hobson's surface analyser. It is suggested that the synthesized coatings display physicochemical and surface properties suitable for materials used for implant.
