RESUMO
A total of 2087 Pseudomonas aeruginosa isolates collected during the period 1994-1997 were used as starting material. Out of 1704 in-patient isolates, 299 strains were selected for the preparation of phage lysates but only five strains provided stable lysates, i.e., maintained the ability to be repeatedly and completely lysed by the appropriate phage in the course of several years. A set of 193 out-patients (189) and water sources (4) isolates failed to yield strains suitable for phage lysate preparation; 190 strains isolated abroad from patients with cystic fibrosis or respiratory infections included three isolates which, despite having a high degree of mucus production, were suitable for lysate preparation. The antigenic pattern of the phage lysates was ascertained by SDS-polyacrylamide gel electrophoresis.
Assuntos
Antígenos de Bactérias/isolamento & purificação , Fagos de Pseudomonas/fisiologia , Pseudomonas aeruginosa/virologia , Tipagem de Bacteriófagos , Eletroforese em Gel de Poliacrilamida , Humanos , Fagos de Pseudomonas/classificação , Pseudomonas aeruginosa/imunologiaRESUMO
A new bacteriophage typing set, composed of 22 phages, was established as a tool for differentiation of Proteus strains. All the phages were tailed and included 4 morphological types (A1, A2, B1 and C1). They were classified into the families Myoviridae, Siphoviridae and Podoviridae. From the set, 19 phages had double-stranded DNA and 3 were single-stranded DNA phages.
Assuntos
Tipagem de Bacteriófagos/métodos , Caudovirales/classificação , Proteus mirabilis/classificação , Proteus mirabilis/virologia , Proteus vulgaris/classificação , Proteus vulgaris/virologia , Caudovirales/genética , Caudovirales/ultraestrutura , DNA de Cadeia Simples/genética , DNA de Cadeia Simples/isolamento & purificação , DNA Viral/genética , DNA Viral/isolamento & purificação , Genoma Viral , Humanos , Microscopia Eletrônica , Myoviridae/classificação , Myoviridae/genética , Myoviridae/ultraestrutura , Podoviridae/classificação , Podoviridae/genética , Podoviridae/ultraestrutura , Proteus mirabilis/isolamento & purificação , Proteus vulgaris/isolamento & purificação , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/ultraestruturaRESUMO
The metabolic activity (oxygen radical formation) of human phagocytes was not substantially affected by the tested cephalosporins. Therapeutic concentrations caused only a mild suppression or immunopotentiation in some cases or there were no effects altogether.
Assuntos
Cefalosporinas/farmacologia , Fagócitos/efeitos dos fármacos , Explosão Respiratória/efeitos dos fármacos , Cefotaxima/farmacologia , Cefsulodina/farmacologia , Ceftazidima/farmacologia , Ceftriaxona/farmacologia , Humanos , Técnicas In Vitro , Cinética , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Oxirredução , Fagócitos/metabolismoRESUMO
Phagocyte released reactive oxygen species are often discussed in connection with ischemic and reperfusion injuries to the myocardium. The kinetics of the accumulation and oxidative burst of human blood phagocytes was studied by chemiluminescence during open heart surgery in the myocardium of human patients. Direct evidence is presented for an accumulation of neutrophils along with their markedly increased metabolic activity (oxygen radical formation), especially following the reperfusion of the ischemic myocardium. Leukocyte numbers and activity remained significantly elevated even in the venous blood obtained 24 h after the operation.
Assuntos
Procedimentos Cirúrgicos Cardíacos , Neutrófilos/fisiologia , Movimento Celular/fisiologia , Feminino , Hematócrito , Humanos , Contagem de Leucócitos , Medições Luminescentes , Luminol , Masculino , Pessoa de Meia-Idade , Miocárdio/metabolismo , Neutrófilos/citologia , Oxigênio/metabolismo , Fagocitose , Traumatismo por Reperfusão/sangue , Traumatismo por Reperfusão/fisiopatologia , Fatores de TempoRESUMO
Studies were made on the morphological variety in plaques produced by phage lysis and autoplaques in Pseudomonas aeruginosa strains. The great variability of phage plaque morphology may lead to a confusion with the autoplaques produced spontaneously by P. aeruginosa strains. The production of autoplaques is characteristic of a large number of clinical strains of P. aeruginosa. The appearance of autoplaques may complicate analysis of significant clinical strains of P. aeruginosa.
Assuntos
Bacteriófagos/crescimento & desenvolvimento , Pseudomonas aeruginosa/metabolismo , Pseudomonas/metabolismo , Ensaio de Placa ViralRESUMO
Genetic mapping of the chromosome of the bacteriophage PS8 of Agrobacterium tumefaciens using temperature sensitive (ts) mutants of the bacteriophage is described.
