RESUMO
A simple procedure was used for the concentration and partial purification of coxsackievirus B3 (Nancy strain). For a large-scale production of virus. Vero cells grown in roller bottles were used. Virus concentrate from a large volume of cell culture supernatant was prepared by precipitation with 6% (w/w) polyethylene glycol. This crude antigen was further purified by banding in cesium chloride gradient using ultracentrifugation. The infectivity and haemagglutination activity of virus were checked up during the whole procedure and the final recovery of infections virus was about 60%.
Assuntos
Antígenos Virais/isolamento & purificação , Enterovirus Humano B/isolamento & purificação , Animais , Centrifugação com Gradiente de Concentração , Meios de Cultura , Enterovirus Humano B/imunologia , Testes de Hemaglutinação , Polietilenoglicóis , Células Vero/microbiologiaRESUMO
Groups of volunteers given intramuscularly 5 or 6.5 dex or perorally 6.5 dex newborn mouse icLD50 of the plaquesegregated "14" clone of the Langat E5 virus strain (tick-borne encephalitis comples) were followed for periods of 12 and 24-27 months. Circulating specific virus neutralizing antibodies persisted in them in the absence of apparent reaction as evidenced by clinical, electroencephalographic and cerebrospinal fluid findings.
