Keratoconus progression after intrastromal corneal ring segment implantation according to age: 5-year follow-up cohort study.

BACKGROUND/AIMS: Intrastromal corneal ring segments (ICRS) have been found to be useful in correcting keratoconus by decreasing irregular astigmatism, thereby potentially improving visual acuity. However, its long-term effects in keratoconus progression are not completely understood, mainly concerning the effects of age on ICRS implantation results. This study aimed to evaluate long-term effects of ICRS implantation according to age at implantation. METHODS: We conducted a longitudinal retrospective study, where we evaluated patients with keratoconus who underwent ICRS implantation between 2004 and 2012. RESULTS: We evaluated 34 eyes for 5 years post-operatively. The mean age of the 21 men and 7 women was 20.59 ± 4.65 years. Best spectacle-corrected visual acuity (BSCVA) improved from 0.32 ± 0.19 in the preoperative period to 0.46 ± 0.27 6 months post-operatively. After up to 5 years of follow-up, there was no significant difference in mean visual acuity of the group. As expected, there was a significant decrease in keratometric values after corneal ring implantation, which remained stable over the 5-year follow-up. Among the 34 cases analysed, nine (26%) showed signs of disease progression. In eight of the nine documented progression cases, patients were 21 years old or younger, revealing that these patients were sevenfold more likely to progress than those aged over 21 years. CONCLUSION: In our series of cases, ICRS implantation was shown to be an excellent treatment to reduce corneal curvature and improve visual acuity at all ages, but it did not stabilize the disease, especially in young patients with more aggressive forms of the keratoconus.

Quercetin pentaacetate inhibits in vitro human respiratory syncytial virus adhesion.

Human respiratory syncytial virus (hRSV) is one of the main etiological agents of diseases of the lower respiratory tract and is often responsible for the hospitalization of children and the elderly. To date, treatments are only palliative and there is no vaccine available. Natural products show exceptional structural diversity and they have played a vital role in drug research. Several investigations focused on applied structural modification of natural products to improved metabolic stability, solubility and biological actions them. Quercetin is a flavonoid that presents several biological activities, including anti-hRSV role. Some works criticize the pharmacological use of Quercetin because it has low solubility and low specificity. In this sense, we acetylated Quercetin structure and we used in vitro and in silico assays to compare anti-hRSV function between Quercetin (Q0) and its derivative molecule (Q1). Q1 shows lower cytotoxic effect than Q0 on HEp-2 cells. In addition, Q1 was more efficient than Q0 to protect HEp-2 cells infected with different multiplicity of infection (0.1-1 MOI). The virucidal effects of Q0 and Q1 suggest interaction between these molecules and viral particle. Dynamic molecular results suggest that Q0 and Q1 may interact with F-protein on hRSV surface in an important region to adhesion and viral infection. Q1 interaction with F-protein showed ΔG= -14.22 kcal/mol and it was more stable than Q0. Additional, MTT and plate assays confirmed that virucidal Q1 effects occurs during adhesion step of cycle hRSV replication. In conclusion, acetylation improves anti-hRSV Quercetin effects because Quercetin pentaacetate could interact with F-protein with lower binding energy and better stability to block viral adhesion. These results show alternative anti-hRSV strategy and contribute to drug discovery and development.

Efeito da idade, do sexo, da altura e do índice de massa corporal no tempo de sucção oral de líquido / Effect of age, gender, height and body mass index on time of oral liquid suction

OBJETIVO: Avaliar o efeito da idade, do sexo, da altura e do índice de massa corporal (IMC) no tempo de sucção oral de água em pessoas normais. MÉTODO: O método constou de, em duplicata, cronometrar o tempo de sucção contínua de 40 ml de água à temperatura ambiente, utilizando um canudo com 6 mm de diâmetro e outro com 3 mm de diâmetro. Cada voluntário foi avaliado sentado, com a cabeça formando um ângulo de 90º entre a mandíbula e o pescoço. Foi permitida a deglutição. Em um grupo de 15 indivíduos, foi avaliada a reprodutibilidade do teste em quatro dias diferentes. O trabalho incluiu 180 indivíduos, o grupo jovem (20 a 30 anos, 45 homens e 45 mulheres) e o grupo idoso (65 a 89 anos, 45 homens e 45 mulheres). RESULTADOS: Observou-se diferença significativa no tempo de sucção entre jovens e idosos, sendo maior para os idosos do que para os jovens. Com o canudo de menor diâmetro, as mulheres idosas tiveram maior tempo de sucção do que os homens idosos. Em mulheres jovens, foi encontrada correlação positiva entre o IMC e o tempo de sucção. Não se observou influência da altura. A repetição do estudo em quatro dias mostrou o mesmo resultado. CONCLUSÃO: Concluímos que o envelhecimento compromete a sucção de líquido, e que este comprometimento é maior nas mulheres do que nos homens. Em mulheres jovens, maior IMC está associado ao maior tempo de sucção.

OBJECTIVE: To assess the effect of age, gender, height and body mass index (BMI) on oral water suction. METHOD: During the test, performed in duplicate, the time one person needs to continuously sip 40 ml of water was measured at room temperature using a straw 6 mm in diameter and another straw 3 mm in diameter. The volunteers were asked to remain seated with their head in a 90º angle between jaw and neck. If necessary they could swallow. In order to verify the reproducibility of the test the same evaluation was conducted in a group of 15 individuals, four times on separate days. The investigation included 180 asymptomatic individuals, a younger group (aged 20 to 30 years, 45 men and 45 women) and an older group (aged 65 to 89 years, 45 men and 45 women). RESULTS: Sucking time was longer for elderly than for young individuals. There was a gender difference for older subjects when the 3 mm straw was used, with a longer time for women than for men. Intra-subject variation was not significant. In young women BMI showed positive correlation with time of suction. Time of suction was not associated with height. CONCLUSION: We concluded that the ageing process causes impairment of liquid suction, and that this impairment is more intense in women than in men. In young women an increase in BMI is associated with a longer liquid suction time.