RESUMO
On the basis of the evidence that the basolateral amygdala (BLA) modulates hippocampal memory processes via synaptic plasticity, here we report that adrenergic ß receptor activation in the BLA rescues amyloid ß1-42 (Aß1-42)-induced attenuation of long-term potentiation (LTP) at perforant pathway-dentate granule cell (DGC) synapses. When 500⯵M isoproterenol (2⯵l), an adrenergic ß receptor agonist, was injected into the BLA 20â¯min before LTP induction, LTP was enhanced. Isoproterenol-mediated enhancement of LTP was blocked by co-injection with 100⯵M ZnAF-2DA, an intracellular Zn2+ chelator, suggesting that intracellular Zn2+ is required for the intracellular signaling cascade after adrenergic ß receptor activation in the BLA. Aß1-42-induced attenuation of LTP, which was induced by Aß1-42 injection into the dentate gyrus 60â¯min before LTP induction, was rescued by isoproterenol injection into the BLA 20â¯min before LTP induction, but not by 500⯵M phenylephrine (2⯵l), an adrenergic α1 receptor agonist, injection into the BLA, which did not enhance LTP unlike the case of isoproterenol injection. Interestingly, Aß1-42-induced attenuation of LTP was also rescued by 100⯵M isoproterenol injection into the BLA 20â¯min before LTP induction, which did not enhance LTP. The present study demonstrates that adrenergic ß receptor activation in the BLA, which is linked with intracellular Zn2+ signaling, rescues Aß1-42-induced attenuation of dentate gyrus LTP. It is likely that adrenergic ß receptor activation in the BLA is a strategy for rescuing Aß1-42-induced cognitive decline that is associated with hippocampal synaptic plasticity.
Assuntos
Quelantes/farmacologia , Giro Denteado/efeitos dos fármacos , Receptores Adrenérgicos beta 1/metabolismo , Sinapses/efeitos dos fármacos , Peptídeos beta-Amiloides/farmacologia , Animais , Complexo Nuclear Basolateral da Amígdala/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Potenciação de Longa Duração/efeitos dos fármacos , Potenciação de Longa Duração/fisiologia , Masculino , Memória/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Fragmentos de Peptídeos/farmacologia , Ratos Wistar , Receptores Adrenérgicos beta 1/efeitos dos fármacosRESUMO
Hippocampus-dependent memory is modulated by the amygdala. However, it is unknown whether intracellular Zn2+ signaling in the amygdala is involved in hippocampus-dependent memory. On the basis of the evidence that intracellular Zn2+ signaling in dentate granule cells (DGC) is necessary for object recognition memory via LTP at medial perforant pathway (PP)-DGC synapses, the present study examined whether intracellular Zn2+ signaling in the amygdala influences object recognition memory via modulation of LTP at medial PP-DGC synapses. When ZnAF-2DA (100 µM, 2 µl) was injected into the basolateral amygdala (BLA), intracellular ZnAF-2 locally chelated intracellular Zn2+ in the amygdala. Recognition memory was affected when training of object recognition test was performed 20 min after ZnAF-2DA injection into the BLA. Twenty minutes after injection of ZnAF-2DA into the BLA, LTP induction at medial PP-DGC synapses was attenuated, while LTP induction at PP-BLA synapses was potentiated and LTP induction at BLA-DGC synapses was attenuated. These results suggest that intracellular Zn2+ signaling in the BLA is involved in BLA-associated LTP and modulates LTP at medial PP-DGC synapses, followed by modulation of object recognition memory.