RESUMO
The article presents the results of the study C677T and A1298C polymorphisms of MTHFR gene and their influence on plasma homocysteine levels in patients with nonalcoholic fatty liver disease (NAFLD). The study involved 100 patients with NAFLD and 40 apparently healthy individuals (control group). Determination of allelic polymorphism was performed by polymerase chain reaction with the detection results of hybridization by fluorescence in real-time. Determination of plasma homocysteine levels was performed by ELISA. As a result, studies have not found significant differences in the distribution of genotypes investigated C677T and A1298C MTHFR gene between patients with NAFLD and control group. We have detected statistically significant relationship between the level of homocysteine plasma C677T polymorphism of the MTHFR gene in patients with NAFLD and lack of connection with the A1298C polymorphism of the gene MTHFR.
Assuntos
Diabetes Mellitus Tipo 2/genética , Homocisteína/sangue , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Hepatopatia Gordurosa não Alcoólica/genética , Polimorfismo de Nucleotídeo Único , Alelos , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/fisiopatologia , Expressão Gênica , Genótipo , Heterozigoto , Homozigoto , Humanos , Metilenotetra-Hidrofolato Redutase (NADPH2)/metabolismo , Hepatopatia Gordurosa não Alcoólica/complicações , Hepatopatia Gordurosa não Alcoólica/metabolismo , Hepatopatia Gordurosa não Alcoólica/fisiopatologiaRESUMO
The contribution of different forest pathways to the irradiation of inhabitants of the forest settlements after the ChNPP accident is estimated. It has been shown that the long-term negative impact of forest ecosystems contaminated to the rural population requires application of special protective measures for a long time after the ChNPP accident.
Assuntos
Radioisótopos de Césio/análise , Ecossistema , Centrais Elétricas , Lesões por Radiação/prevenção & controle , Proteção Radiológica , Liberação Nociva de Radioativos , Poluentes Radioativos/análise , Humanos , República de Belarus , População Rural , Federação Russa , Fatores de Tempo , UcrâniaRESUMO
The effect of ionizing irradiation on the viability of pine stands after the fallout from the damaged nuclear energy plant at Chernobyl (ChNPP) was shown within the territory of the 10-km zone. During the period 1986-1991, irradiated and damaged forest stands, so-called 'red forest', located in this area were systematically classified by observation. Mortality rate, re-establishment, development of tree canopies, reproduction anomalies and stand viability were shown to be dependent on absorbed irradiation dose, on the age of the stand and on forest composition. For pine stands in the acutely affected zone, doses of more than 60 Gy resulted in a massive mortality and no regeneration of pine trees since 1987. The injured trees had burned or had dried-up. The drying process was accelerated by a massive production of pathogenic insects invading the dying trees. Specifically, irradiation doses of 10-60 Gy, 1-10 Gy and 0.1-1 Gy caused high, medium and low injury to the forest stands, respectively. Doses of less than 0.1 Gy did not cause any visible damage to the trees. In 1987, repair processes were displayed by the tree canopies and practically the entire viability of the forest stands had recovered except for trees in the acute and highly affected zones. The young forest was reestablished in the same place as the perished trees and new pine saplings were planted on the reclaimed areas.
