[Comparison of digital and visual methods for Ki-67 assessment in invasive breast carcinomas]. / Sravnenie tsifrovogo i vizual'nogo metodov dlia otsenki indeksa Ki-67 v invazivnykh kartsinomakh molochnoi zhelezy.

AIM: to compare two methods for quantitative assessment of the proliferative activity index (PAI): a visual estimation method by several investigators and digital image analysis (DIA). The use of the Ki-67 index in the daily clinical practice of a Morbid Anatomy Department is associated with the problem of reproducibility of quantitative assessment of the Ki-67 PAI. Due to the development of digital imaging techniques in morphology, new methods for PAI evaluation using the DIA are proposed. MATERIAL AND METHODS: The Ki-67 PAI data obtained during visual assessment and digital image analysis were compared in 104 cases of grades 2-3 breast carcinoma. The histological sections were scanned using a Panoramic III scanner (3D Histech, Hungary) and digital images were obtained. DIA was carried out using the software 3D Histech QuantCenter (3D Histech, Hungary), by marking 3-10 zones. Evaluation of the obtained sections was done independently by two investigators engaged in cancer pathology. RESULTS: The level of agreement between visual and digital methods did not differ significantly (p>0.001). The authors selected a gray area in the range of 10-35% IPA, where the Ki-67 index showed a weak relationship between the analyzed groups (ICC, 0.47). The Ki67 index below 10% and above 35% showed a sufficient reproducibility in the same laboratory. CONCLUSION: The authors consider that the scanned digital form of a histological section, which can be evaluated using automated software analysis modules, is an independent and objective method to assess proliferative activity for Ki-67 index validation.

[The Paris system of interpretation of urinary cytopathology.]

The article presents the Paris system of interpretation of urinary cytopathology developed by joint efforts of cytologists, pathologists and urologists in April 2016. The seven accepted categories of cytological opinion letters are presented, including criteria of their evaluation, risk of malignancy development under various urinary pathology and recommended tactics of management of patients.