[Effect of cryopreserved cord blood nucleated cells on course of pathological processes in progressive aging of brain (experimental study).]

The data on morphometric indices of the vascular bed and brain substance of spontaneously hypertensive SHR rats, which can be considered as an evidence of the developing pathological brain aging processes in these animals, are presented in the work. Chronic alcohol intoxication aggravates the neurodegeneration, significantly reducing the indices of the neuroglial index, the number of functioning vessels and activating the LPO processes. Single intraperitoneal administration of cryopreserved human cord blood nucleated cells in a dose of (1-5)‧107/kg promoted the regeneration of neurocells, stimulation of angiogenesis and an increased level and quality of neurotissue metabolism, reducing the signs of dystrophic, destructive and pathologically altered compensatory adaptive processes in the rat's brain as a result of the restoration of its microhemocirculation and cytoarchitecture.

[The effect of initial bioenergetic state on the cryostability of yeast cells]. / Vliianie iskhodnogo sostoianiia bioénergetiki na kriostabil'nost' drozhzhevykh kletok.

The cryostability of Saccharomyces cerevisiae cells decreased when they were cultivated under anaerobic conditions in a liquid growth medium YEPD as compared to the culture grown under aerobic conditions. The effect of cultivation conditions on the different cryostability of S. cerevisiae cells is discussed. The initial state of their bioenergetics was shown to influence the cryostability of yeast cells.

[Cryostability of yeast cells and their sensitivity to basic factors of freezing]. / kriostabil'nost'' drozhzhevykh kletok i ikh chuvstvitel'nost' k osnovnym faktoram zamorazhivaniia.

The cryostability of Saccharomyces cerevisiae cells was found to correlate with their sensitivity to some physico-chemical factors of freezing. The threshold of their resistance to the main factors of cryoconservation (a low-temperature shock, the presence of a salt, a change in the pH of the medium) was determined at a temperature value physiological for this microorganism. This work was done in order to substantiate and predict optimal freezing regimes while making microbial cells pass into the state of deep cryptobiosis.