Phenyl Propionate and Sex Pheromone for Monitoring Navel Orangeworm (Lepidoptera: Pyralidae) in the Presence of Mating Disruption.

The recent availability of sex pheromone lures for the navel orangeworm, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), improves options for monitoring this key pest in conventionally managed almonds. These lures are, however, minimally effective in the presence of mating disruption. Experiments were conducted to determine if phenyl propionate (PPO), an attractant for the navel orangeworm, acts in an additive or synergistic manner when presented together with the pheromone. In the absence of mating disruption, traps baited with PPO captured significantly fewer adults than traps baited with a sex pheromone lure. There was no difference in the number of adults captured in traps with both attractants when mating disruption was not used. In the presence of mating disruption, pheromone traps were completely suppressed, yet traps with both pheromone and PPO captured significantly more adults than traps baited with only PPO. Traps with only PPO captured equal numbers of both sexes, whereas traps with both attractants had significantly more males. These findings demonstrate that PPO is likely to be useful for monitoring navel orangeworm in fields treated with mating disruption.

Measure your septa release ratios: pheromone release ratio variability affected by rubber septa and solvent.

The type of solvent and the volume used to load pheromone components onto rubber septa had significant effects on pheromone release ratios, the variability of those release ratios, and the recoverability of the volatile components during subsequent extraction with hexane. Volatile release ratios of synthetic Oriental fruit moth (OFM) pheromone and additional volatile compounds were determined using a gas chromatograph column as a volatile trap for rapid (≤1 hr) analysis from individual rubber septa. Volatile compound solutions were prepared in hexane, pentane, CH2Cl2, and methyl tert-butyl ether, and a 10, 33, or 100 µl aliquot of each solution was applied to rubber septa. Septa loaded with 100 µl of CH2Cl2 emitted significantly (P < 0.05) higher alcohol: acetate (OH:Ac) ratios than septa loaded with the other solvents, which were all similar. Release ratios of the alcohol and acetate components of the OFM pheromone components were assessed over a 3 week period using septa loaded with each solvent. Regardless of loading solvent, the OFM OH:Ac ratios declined logarithmically over 3 weeks; however, the decay slope from septa loaded with CH2Cl2 solutions was different from those of the other three solvents, which were nearly all the same. A high variability in OH:Ac release ratios was measured overall, regardless of the solvent used or the volume it was applied in. Four compounds of near-equal mass: 1-dodecanol, 1-dodecanal, methyl decanoate, and tridecane emitted different release ratios dependent on the solvent, hexane or CH2Cl2, with which a septum was loaded. The more polar and the greater the mass of the test compound, the slower it was emitted from a septum regardless of solvent. These combined results plus comparisons to earlier reports, suggest that researchers should empirically assess the release ratios from septa to be used in bioassays rather than just reporting the type of septum, ratios of compounds applied and solvent used to prepare them.

Variable developmental rate and survival of navel orangeworm (Lepidoptera: Pyralidae) on pistachio.

A series of laboratory and field studies were conducted using two lines of navel orangeworm, reared on different stages of new crop and mummy pistachios, Pistacia vera L. This study demonstrated the potential importance of malformed pistachios (pea splits) to the population dynamics of navel orangeworm, because these nuts, which are available as early as two months before mature nuts, supported navel orangeworm development and survival. Overall, the developmental rate on new crop pistachios is fastest on mature nuts, 422.3 +/- 123 degree-days (DD, degrees C), but other factors such as exposure to insecticide residue also sped development, although survival decreased. Development took the longest on unharvested nuts (mummies) dried at 90 degrees C for 24 h, 2664.7 +/- 131.4 DD. In most trials development was variable and two generations could develop at the fastest rate before the slowest individual completed development, which in turn calls into question the concept of discrete generations. Generally, survival was highest on mature pistachios and other stages of new crop nut and lowest on mummies collected in May. Survival was also higher on the new varieties 'Lost Hills' and 'Golden Hills' (24.7 and 32.0%, respectively) than on the most extensively planted variety 'Kerman' (13.3%). In our trials, both the rate of development and survival were dependent on nut stage, age, variety, and quality, indicating that pistachios, like almonds, Prunus dulcis (Mill.) D. A. Webb, are a dynamic rather than a static nutrient source for navel orangeworm.

Variable development rate and survival of navel orangeworm (Lepidoptera: Pyralidae) on wheat bran diet and almonds.

A series of laboratory and field studies were conducted using three lines of navel orangeworm, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), reared on wheat bran diet and almonds, Prunus dulcis (Mill.) D.A.Webb, at constant and fluctuating temperature. The duration of development on wheat bran diet at constant temperature differed significantly among the three lines. Development was as much as 40% faster at constant temperature than at fluctuating temperatures, consequently the developmental duration determined at constant temperature was not an absolute measure. When the maximum temperature in fluctuating regimes exceeded 43 degrees C, survival decreased by 50% compared with the constant temperature control. In almonds held at constant temperature, the developmental rate on new-crop nuts was variety-dependent and was fastest on 'Nonpareil' almonds and slowest on the experimental selection '23-122'. Development and survival were also variety-dependent on unharvested (mummy) almonds, and navel orangeworm average emergence was earliest from Nonpareil and latest from 'Carmel' nuts, differing by 529 degree-days, whereas survival was the highest on 'Butte', 35.7%, and the lowest on Carmel nuts, 7.2%. In our trials, both the speed of development and survival depended on host age, variety and quality, indicating that almonds were a dynamic rather than a static nutrient source for navel orangeworm. Identifying the factors responsible for variation in development and survival will give insight into improving control strategies.

Attractiveness of a four-component pheromone blend to male navel orangeworm moths.

The attractiveness to male navel orangeworm moth, Amyelois transitella, of various combinations of a four-component pheromone blend was measured in wind-tunnel bioassays. Upwind flight along the pheromone plume and landing on the odor source required the simultaneous presence of two components, (11Z,13Z)-hexadecadienal and (3Z,6Z,9Z,12Z,15Z)-tricosapentaene, and the addition of either (11Z,13Z)-hexadecadien-1-ol or (11Z,13E)-hexadecadien-1-ol. A mixture of all four components produced the highest levels of rapid source location and source contact. In wind-tunnel assays, males did not seem to distinguish among a wide range of ratios of any of the three components added to (11Z,13Z)-hexadecadienal. Dosages of 10 and 100 ng of the 4-component blend produced higher levels of source location than dosages of 1 and 1,000 ng.

Identification of critical secondary components of the sex pheromone of the navel orangeworm (Lepidoptera: Pyralidae).

We identified a four-component sex pheromone blend that is as attractive or more attractive to male navel orangeworm moths, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), than either unmated females or hexane extracts of pheromone glands of females. The blend consisted of (11Z,13Z)-hexadecadienal; (11Z,13Z)-hexadecadien-1-ol; (11Z,13E)-hexadecadien-1-ol; and a hydrocarbon, (3Z,6Z,9Z,12Z,15Z)-tricosapentaene (C23 pentaene), in ratios of approximately 100:100:5:5. Other minor components of pheromone gland extracts included (11Z,13E)-hexadecadienal; (11E,13Z)-hexadecadienal; (11Z,13Z)-hexadecadien-1-yl acetate; (Z)-11-hexadecenal; hexadecanal; hexadecan-1-ol; and a second pentaene, (3Z,6Z,9Z,12Z,15Z)-pentacosapentaene (C25 pentaene). These minor components did not increase attraction of male navel orangeworm to the basic four-component blend. The use of another, cross-attracted pyralid moth, Pyralisfarinalis L., as a model species was crucial in implicating the C23 pentaene as an important component of the navel orangeworm pheromone blend. The four-component navel orangeworm pheromone blend was optimized using a combination of wind tunnel and field bioassays. Attractiveness of field deployed synthetic pheromone lures decreased rapidly despite incorporation of stabilizers and use of different release devices, suggesting that degradation products antagonize male navel orangeworm responses. Overall, the combination of type I lepidopteran pheromone components consisting of C16 aldehydes and alcohols with type II components consisting of long-chain polyunsaturated hydrocarbons has now been documented in several lepidopteran species and may indicate a paradigm shift in the range of compounds that constitute sex pheromone blends for individual lepidopteran species. This suggests that careful reexamination of pheromone gland contents for both type I and type II compounds may prove fruitful in species that have been studied but for which full attractant blends have eluded identification.

Protracted emergence of overwintering Amyelois transitella (Lepidoptera: Pyralidae) from pistachios and almonds in California.

The navel orangeworm, Amyelois transitella (Walker), is the primary insect pest of pistachios and almonds in California. Four years of research (2002-2006) were conducted in Madera and Kern Counties to elucidate the pattern of adult emergence of the overwintering navel orangeworm population. Springtime emergence from unharvested (mummy) nuts was protracted (600 degree-days or more from 1 January of each year) and in 2004 and 2006 extended to mid-July. The population structure, sex ratio, and timing of emergence differed between pistachio and almond mummies. Pistachio populations had a significantly greater proportion of late stage individuals compared with almond mummies, 85.7 versus 34.1%. The sex ratio of adults emerging from pistachio mummies was significantly skewed with a ratio 57:43 male:female compared with 50:50 in almond mummies. Emergence from mummies held outdoors (variable temperature) began in early March and continued through early June in both pistachio mummies and almond mummies. The adult emergence pattern from pistachio mummies contained a single emergence peak, whereas emergence from almond mummies occurred in multiple peaks. These same patterns occurred when mummies were held at constant temperature, and the emergence peak from pistachio mummies occurred sooner. The impact of these findings on understanding navel orangeworm population dynamics and current control recommendations is discussed.

Multimodal integration: visual cues help odor-seeking fruit flies.

Olfactory stimuli are uniquely devoid of directional information, so that multimodal cues are typically required for their localization. A clever new experimental paradigm with flying Drosophila has shown that accurate heading into an odor plume requires panoramic visual cues.

Effects of western honey bee (Hymenoptera: Apidae) colony, cell type, and larval sex on host acquisition by female Varroa destructor (Acari: Varroidae).

Female mites of the genus Varroa reproduce on the immature stages of Apis cerana F. and A. mellifera L. Mites are found more often in drone brood than worker brood, and while evolutionary explanations for this bias are well supported, the proximate mechanisms are not known. In one experiment, we verified that the proportion of hosts with one or more mites (MPV, mite prevalence value) was significantly greater for drones (0.763 +/- 0.043) (lsmean +/- SE) than for workers (0.253 +/- 0.043) in populations of mites and bees in the United States. Similar results were found for the average number of mites per host. In a second experiment, using a cross-fostering technique in which worker and drone larvae were reared in both worker and drone cells, we found that cell type, larval sex, colony and all interactions affected the level of mites on a host. Mite prevalence values were greatest in drone larvae reared in drone cells (0.907 +/- 0.025), followed by drone larvae reared in worker cells (0.751 +/- 0.025), worker larvae reared in worker cells (0.499 +/- 0.025), and worker larvae reared in drone cells (0.383 +/- 0.025). Similar results were found for the average number of mites per host. Our data show that mite levels are affected by environmental factors (cell type), by factors intrinsic to the host (sex), and by interactions between these factors. In addition, colony-to-colony variation is important to the expression of intrinsic and environmental factors.

Identification of sex pheromone component of spruce budmothZeiraphera canadensis.

The analyses of virgin female sex pheromone gland extracts by gas chromatography (GC), GC-electroantennographic detection (GC-EAD) and GC-mass spectrometry (GC-MS) followed by field-trapping experiments, have identified (E)-9-tetradecenyl acetate (E9-14:Ac) as the primary sex pheromone component of the spruce budmoth,Zeiraphera canadensis. Dosages of 1.0-100.0 [Symbol: see text]g ofE9-14:Ac impregnated in rubber septa provide effective trap baits.

Sex pheromone of purplestriped shootworm,Zeiraphera unfortunana powell.

The analyses of virgin female sex pheromone gland extracts and gland volatiles by GC, GC-EAD and GC-MS, followed by field trapping experiments, have identified (E)-9-dodecenyl acetate (E9-12∶Ac) as the primary sex pheromone component of the purplestriped shootworm,Zeiraphera unfortunana. Dosages of 1.0-10.0 µg ofE9-12∶Ac impregnated in rubber septa provide an effective trap bait and can be used for monitoring purposes.

Spruce budworm (Choristoneura fumiferana) pheromone chemistry and behavioral responses to pheromone components and analogs.

This paper reviews the sex pheromone chemistry and pheromone-mediated behavior of the spruce budworm and related coniferophagous (Choristoneura) budworms. InC. fumiferana, temporal changes in pheromone-gland monounsaturated fatty acids (pheromone precursors) enable the prediction of the primary sex pheromone components. This technique may also be applicable for predicting additional pheromone components. Tetradecanal (14∶ Ald), previously shown to enhance close-range precopulatory behavior, lowers the threshold of response by males for upwind flight to a pheromone-component source. Spruce budworm males maintain upwind flight to 95∶5 (E/Z)-1,12-pentadecadiene (diolefin analog) after initiating upwind flight to a primary-component pheromone source (95∶5E/Z11-14∶Ald). This is the first demonstration of apparently normal male flight responses to a pheromone analog.

Identification of sex pheromone components of jack pine budworm,Choristoneura pinus pinus freeman.

Chemical identification and field-trapping experiments have shown that a blend of 85∶15 (E,Z)-11-tetradecenyl acetates and 85∶15 (E,Z)-11-tetradecen-1-ols (in a 9∶1 ratio) are female sex pheromone components for jack pine budworm,Choristoneura pinus pinus. This blend of chemicals, formulated in PVC (0.1 %, w/w) sources is as effective a trap bait as virgin females. Preliminary wind tunnel observations have indicated that this blend, effective as a trap bait, is not equivalent to females.

Pheromone source location by flying moths: a supplementary non-anemotactic mechanism.

After the wind was stopped in an insect flight tunnel, male oriental fruit moths continued to fly in zigzag fashion along a stationary pheromone plume. Their lateral excursions from the time-averaged pheromone plume were no greater without wind than in wind of 38 centimeters per second. When the pheromone plume was removed and the wind stopped, males initiated wider track reversals when they reached the pheromone-free area in still air than they had made while in the pheromone plume. This non-anemotactic mechanism of maintaining plume contact-possibly a special kind of klinotaxis-when coupled with the orthokinetic retinal velocity of apparent ground pattern motion, allowed males to reach the pheromone source area from 1 to 2 meters away without wind.

A high-efficiency collection device for quantifying sex pheromone volatilized from female glands and synthetic sources.

A high-efficiency collection device for sex pheromones volatized from forcibly extruded female glands is described. Filtered nitrogen gas is the carrier and glass wool the adsorbent. Small quantities of distilled carbon disulfide are used to rinse the glass wool. Recovery efficiency of synthetic compounds was usually 90-100%, and a mean of 2.4 ± 0.65 SD ng/min of (Z)-7-dodecenyl acetate was recovered in emissions from individualTrichoplusia ni (Hubner) glands.