Assuntos
Doenças Autoimunes/virologia , Doenças do Tecido Conjuntivo/virologia , DNA Viral/análise , Herpesvirus Humano 4/fisiologia , Saliva/virologia , Eliminação de Partículas Virais , Adulto , Idoso , Anticorpos Antivirais/sangue , Antígenos Virais/imunologia , Proteínas do Capsídeo/imunologia , Estudos de Casos e Controles , Infecções por Vírus Epstein-Barr/complicações , Antígenos Nucleares do Vírus Epstein-Barr/sangue , Feminino , Herpesvirus Humano 4/imunologia , Humanos , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-Idade , Gravidade do Paciente , Projetos Piloto , Recidiva , Saliva/química , Ativação Viral , Adulto JovemAssuntos
Anticorpos Monoclonais/efeitos adversos , Antirreumáticos/efeitos adversos , Toxidermias/etiologia , Imunoglobulina G/efeitos adversos , Psoríase/induzido quimicamente , Artrite Reumatoide/tratamento farmacológico , Etanercepte , Feminino , Humanos , Infliximab , Pessoa de Meia-Idade , Receptores do Fator de Necrose TumoralAssuntos
Herpes Zoster/complicações , Herpes Zoster/diagnóstico , Linfoma de Células B/complicações , Aciclovir/análogos & derivados , Aciclovir/uso terapêutico , Idoso , Antivirais/uso terapêutico , Herpes Zoster/tratamento farmacológico , Humanos , Masculino , Pele/patologia , Coxa da Perna/patologia , Valaciclovir , Valina/análogos & derivados , Valina/uso terapêutico , Vidarabina/uso terapêuticoRESUMO
BACKGROUND/PURPOSE: 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) is widely performed in the clinical setting for superficial skin cancers, giving favorable results, but residual tumor and recurrence occur occasionally. Thioredoxin is a common antioxidant that suppresses apoptosis and facilitates cell growth. We investigated the expression of thioredoxin following ALA-PDT in human skin squamous cell carcinoma cell line, HSC-5. METHODS: ALA-PDT was performed in HSC-5 cells using low-dose (5 J/cm(2), 100 mW/cm(2)) or high-dose (30 J/cm(2), 100 mW/cm(2)) irradiation, and the expression of thioredoxin was measured by Western blotting. An MTT assay was used to assess cell growth following a low dose of multiple irradiations. Cell death was examined by Western blotting for caspase-3 and PARP. Immunofluorescence double staining using annexin V and propidium iodine was also performed. RESULTS: Expression of thioredoxin was only observed following low-dose exposure ALA-PDT. Multiple low-dose exposure ALA-PDT significantly proliferated cell growth. With high-dose exposure ALA-PDT, caspase-3 and PARP expression were seen, and cell death due to apoptosis and/or necrosis was observed, but thioredoxin was barely detected. CONCLUSION: Low-dose exposure ALA-PDT increased the expression of thioredoxin and facilitated the growth of HSC-5 cells.
Assuntos
Ácido Aminolevulínico/farmacologia , Carcinoma de Células Escamosas/metabolismo , Morte Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Tiorredoxinas/metabolismo , Ácido Aminolevulínico/efeitos adversos , Análise de Variância , Western Blotting , Carcinoma de Células Escamosas/tratamento farmacológico , Carcinoma de Células Escamosas/patologia , Caspase 3/metabolismo , Morte Celular/fisiologia , Linhagem Celular Tumoral , Colágeno Tipo XI/metabolismo , Humanos , Lasers de Corante , Microscopia de Fluorescência , Recidiva Local de Neoplasia/induzido quimicamente , Fotoquimioterapia/efeitos adversos , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/efeitos adversos , Doses de Radiação , Regulação para Cima/efeitos dos fármacosRESUMO
The reliability of a loop-mediated isothermal amplification (LAMP) method for the detection of human herpesvirus 8 (HHV-8) DNA was evaluated. Although LAMP products were produced with the DNA sample extracted from BCP-1 cells, LAMP products were not produced with the DNAs from seven other human herpesviruses. The detection limit of the HHV-8 LAMP method was 100 copies of target sequence/tube. To determine whether the HHV-8 LAMP method could be used to quantify viral DNA, threshold times, which are defined as the time (in s) it takes to reach the threshold turbidity level (0.1), were measured for the amplification of serial dilutions of a DNA plasmid containing the target sequence. The standard curve possessed a correlation coefficient of 0.9428 with a slope of -84.079 and y-intercept value of 1936.2. Additionally, an attempt was made to detect viral DNA in 17 specimens collected from Kaposi's sarcomas and two cell lines obtained from primary effusion lymphomas. HHV-8 DNA was detected in 14 of the 17 Kaposi's sarcoma tissue samples and both of the primary effusion lymphoma cell lines. Viral DNA was not detected in HHV-8 LAMP-negative samples using the real-time PCR method.
Assuntos
DNA Viral/análise , Herpesvirus Humano 8/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Sarcoma de Kaposi/diagnóstico , Humanos , Sarcoma de Kaposi/virologia , Sensibilidade e EspecificidadeRESUMO
BACKGROUND: Sezary syndrome and mycosis fungoides are forms of cutaneous T-cell lymphoma, and in the early stage of these diseases psoralen plus ultraviolet A (PUVA) is one of the treatments of choice. Photodynamic therapy using 5-aminolevulinic acid (ALA-PDT) is an effective, non-invasive, and safe treatment for most superficial skin cancers. In order to obtain greater efficacy of PUVA, we investigated the synergistic anti-tumor effects of ALA-PDT and PUVA using 8-methoxypsoralen (8-MOP) and a UVA lamp. METHODS: The in vitro effects of PUVA and ALA-PDT and their combination in HUT-78 cell line from human SS were determined by MTT assay. RESULTS: In our results, cell proliferation compared with controls was inhibited to 53.2% with UVA alone, 52.3% with 1 microM 8-MOP, 43.8% with 100 microM ALA, and 19.2% with combined 8-MOP and ALA. CONCLUSION: Combined use of ALA and PUVA using 8-MOP and UVA lamps, which are widespread in Japan, had a strong anti-tumor effect in vitro. Combined treatment with ALA-PDT and PUVA using a UVA lamp appears to have a strong treatment effect.