RESUMO
Bovine monocyte chemoattractant protein-1 (bovine MCP-1) cDNA has recently been characterized and shown to be highly expressed in bovine seminal vesicles. In an attempt to isolate the MCP-1 gene, we screened a bovine genomic lambda EMBL-3 library with an MCP-1 specific probe pH42. A positive clone lambda MCP1 was subjected to restriction analysis and a pH42-positive EcoRI-fragment of 3.8 kb was subcloned and sequenced. The bovine MCP-1 gene consists of three exons separated by two introns. We undertook to identify homologous sequences 5'-upstream of the transcriptional start site within the genes of rat, mouse, human and bovine MCP-1. Six conserved sequence stretches located within 400 bp upstream of the transcriptional start site in MCP-1 genes were detected. Interestingly an approximately 45 bp long region, displaying identities in the range of 68-72% relative to the human sequence, covers in the rat gene the -141/88 region responsible for TPA induction of gene expression.
Assuntos
Fatores Quimiotáticos/genética , Citocinas/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Bovinos , Quimiocina CCL2 , Fatores Quimiotáticos/metabolismo , DNA Complementar , Humanos , Dados de Sequência Molecular , Homologia de Sequência do Ácido NucleicoRESUMO
As part of an attempt to understand the androgen-regulated expression of seminalplasmin, a major basic protein of bovine seminal vesicle secretion, we have characterized the bovine seminalplasmin gene. The compact gene of approximate size of 2.1 kb is organized in four exons and three introns. Regulatory sequences involved in regulation of transcription could not be identified by simple sequence homologies. A putative promoter element TATAA is located 29 bp upstream of exon 1.
