[Effect of iron, zinc, and copper on lipid peroxidation in liver in vivo]. / Vliianie zheleza, tsinka, medi na protsessy perekisnogo okisleniia lipidov pecheni in vivo.

Changes in the levels of lipid peroxides, natural antioxidants (NA), iron, zinc and copper as well as the ability of the lipid substrate to oxidize in animal liver in the presence of iron, zinc and copper have been studied. It has been found that those metals increase the amplitude of natural fluctuations in iron, zinc and copper levels in the liver (by 5-30% in comparison with control). Within the first few hours following administration of iron and copper, the concentration of lipid peroxidation (LPO) products in animal livers increases, while the NA level and lipid oxidizeability decrease, in contrast. Zinc inhibits LPO in the liver within the first few hours following the injection. The changes in the content of NA, lipid oxidizeability and the level of LPO products in animal livers following iron, zinc and copper administration are phase-dependent. Data from regression analysis indicate a direct dependence (kcor = 0.98 at p < or = 0.05) between lipid oxidizeability and NA content in the liver following administration of iron, zinc and copper which, in turn, is suggestive of the lack of disturbances in the system of NA regulation

[Reduction of voluntary alcohol consumption under the effects of prolonged-action zinc]. / Snizhenie dobrovol'nogo potrebleniia étanola pod vliianiem preparata tsinka prolongirovannogo deistviia.

It was revealed that an involuntary rats' intoxication with 10% ethanol solution during 8 months caused a reduction of zinc content in the brain. A subcutaneous depot administration of highly dispersed zinc powder in a dose of 5 mg/kg reduced ethanol consumption under conditions of free choice for two weeks. This effect of the preparation was associated with normalization of zinc content and increase of a malondialdehyde level in the brain.

[The study of the additive antioxidative activity of total natural lipid antioxidants]. / Izuchenie additivnogo antiokislitel'nogo deistviia summy prirodnykh antioksidantov lipidov.

Kinetics of methyl oleate and ethylbenzene oxidation was studied in presence of tocopherol homologues alpha, beta, gamma, delta, tocopherol derivatives C6 and C11, ubichinol QH2-9, which were isolated from natural lipids. Effects of various natural antioxidants in mixtures were interindependent.

[Changes in the physico-chemical characteristics of mouse liver lipids after administration of a mixture of unsaturated fatty acids]. / Izmenenie fiziko-khimicheskikh kharakteristik lipidov pecheni myshei pri vvedenii zhivotnym smesi nenasyshchennykh zhirnykh kislot.

The effects of a mixture of unsaturated fatty acids (UFA) on changes in the lipid oxidation substrate, a system of natural antioxidants and the functioning of a system controlling lipid peroxidation were studied. It was shown that arachidonate induces the incorporation of fatty acids into individual lipid fractions and the dilution of lipids by UFA esters, as a result of which the relative amount of natural antioxidants diminishes. Under these conditions, the oxidative capacity of lipids decreases, which manifests itself in a reduction of the rate of natural antioxidants utilization and an increase in their number. It was shown that purposeful modification of the lipid substrate does not interfere with the interaction between individual parameters of the system controlling lipid peroxidation.

[Rate of enzymatic lipid peroxidation of mouse liver microsomes after the administration of a highly-dispersed iron powder]. / Intensivnost' fermentativnogo perekisnogo okisleniia lipidov mikrosom pecheni myshei posle vvedeniia vysokodispersnogo poroshka zheleza.

It was found that the rate of the NaDPH-dependent lipid peroxidation in rat microsomes in vitro decreases 3 hours and increases 24, 48 and 72 hours after the injection of a highly dispersed iron powder (2 mg/kg). The number of readily oxidizable phospholipid fractions (phosphatidylethanolamine, phosphatidylinositol) was shown to decrease 3 hours and to increase 24 hours following the injection. A direct correlation was observed between the activity of the NADPH-dependent microsomal lipid peroxidation system and the level of natural antioxidants in the lipids.

[Effect of lateral wick chain of tocopherols on oxidative reactions in lipids]. / O vliianii bokovoi fitil'noi tsepi tokoferolov na okislitel'nye reaktsii, protekaiushchie v lipidakh.

An injection of a alpha-tocopherol and analog devoid of the lateral chain (C1) at a dose of 90 mg/kg of body weight to experimental animals increases the levels of natural antioxidants and lipid oxidation in mouse liver 4- and 4.5-fold, respectively. The effects of alpha-tocopherol on the lipids possessing a high antiradical activity and containing the lateral chain and that of its analog, C1, also possessing a high antiradical activity but devoid of the lateral chain, were compared. The similarity of alpha-tocopherol and its analog effects on the lipids can be due to their antiradical properties. The degree of this effect is, however, different, which can be accounted for by possible fixation of the molecular in the membrane due to the presence of the lateral chain and a resulting increase of antioxidant concentration in the lipids.

[Interrelationship between the level of natural antioxidants and lipid oxidation in the normal mouse liver and after injection of alpha-tocopherol]. / Vzaimosviaz' mezhdu kolichestvom prirodnykh antioksidantov i okisliaemost'iu lipidov pecheni myshei v norme i pri vvedenii al'fa-tokoferola.

The changes in the level of natural antioxidants and lipid oxidation in normal mouse liver and after injection of the natural antioxidant alpha-tocopherol were studied. The level of natural antioxidants and lipid oxidation were increased 1--2-fold within 24 hrs; after alpha-tocopherol injection these parameters were increased 37- and 39-fold, respectively. A direct correlation between the amount of natural antioxidants and lipid oxidation in normal mouse liver and after alpha-tocopherol injection was established.

[Effect of small dispersed iron powder on the physico-chemical properties of mouse liver lipids]. / Vliianie melkodispersnogo poroshka zheleza na fiziko-khimicheskie kharakteristiki lipidov pecheni myshei.

The changes in the number of natural antioxidants, antioxidative activity, lipid peroxidation products, composition and oxidizing capacity of mouse liver lipids induced by subcutaneous injection of small dispersed iron powder (2 mg/kg) were investigated. During the first 6 hours following the injection the number of natural antioxidants, the antioxidative activity and lipid-oxidizing capacity are decreased, while the number of lipid hydroperoxides is increased, i.e. Fe exerts prooxidant effects on the lipids. All the changes in the physico-chemical parameters of the lipids under study are phasic and time-consuming. A direct correlation between the changes in the oxidizing capacity of the lipids and the content of natural oxidants in the lipids induced by injections of small dispersed iron poweder was established.

[Chemiluminescent study of the antiradical activity of tocopherol analogs and homologs]. / Izuchenie antiradikal'noi aktivnosti analogov i gomologov tokoferola metodom khemiliuminestsentsii.

Antioxidative peculiarities of the effect of tocopherol derivatives are considered. Attempts are made to reveal interrelation between tocopherol pharmacological effect and antiradical activity of its derivatives exemplified by an elementary reaction of tocopherol interaction with free peroxide radicals (FR). It is shown that the presence of free hydroxyl groups, number and location of CH3--groups in tocopherol benzol ring produce a significant effect on tocopherol ability to react with FR. The length of lateral phitil chain produces no appreciable effect on the rate of tocopherol reaction with free radicals. The values of energy activation in this reaction are calculated for tocopherol derivatives. Correlation between biological and antiradical activity of tocopherol homologs is shown. The absence of such correlation for tocopherol analogs is explained by the difference in the ability of analogs to be incorporated into biological membranes. Possible tocopherol regulations of the rates of free radical processes proceeding in lipid membranes are considered.