Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Klin Lab Diagn ; 64(9): 560-564, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31610109

RESUMO

Human babesiosis caused by parasitic protozoan Babesia spp. is sporadic zoonotic vector-borne infection. The course of babesiosis and prognosis depend on the type of pathogen and on the patient's immunological status. Significance this disease is a severe, often fatal course with immunocompromissed patients resembling complicated falciparum malaria. In Europe to date, more than 50 cases of confirmed human babesiosis have been reported in most cases caused by Babesia divergens. Possible there are unrecognized cases. Pathogen is an obligate intraerythrocyte parasite of vertebrate animals. The organism is transmitted from animal to man through bite of Ixodidae tick. Asexual reproduction of the parasite occurs in a vertebrate host. The pathogenesis of babesiosis is caused by the destruction of host cells. Intensive haemolysis of red blood cells leads to the development of haemolytic anemia, haematuria, jaundice, and polyorgan failure may develop. The clinical manifestations of the disease are nonspecific. Detection of intraerythrocyte parasites in blood smears stained Gimsa-Romanovsky confirms the proposed diagnosis. Blood smears and some laboratory signs from fatal cases were analyzed in the Reference-centre of E. I. Martsinovskii Institute. Original microphotographs B. divergens are shown. The main morphological forms of the parasite are shown. In addition to the well-known tetrades of parasites «Maltese Cross¼, for the first time, the parasites dividing into 6 interconnected trophozoites - "sextet" - were found. Originally, the invasion of Babesia in a normoblast is shown. An unusually high multiple invasion (14 parasites) of erythrocytes is noted. Because the patients, initially, were incorrectly diagnosed with malaria, the differential diagnosis of Babesia with Plasmodium is described step-by-step. It is important, since the treatment with antimalarial drugs is ineffective. Deviation laboratory signs are discussed. Complex morphological characteristics allowed us to speciated the parasites as B. divergens. DNA was detected in the sample with specific primers Bab di hsp70F/Bab di hsp70R and the probe Bab di hsp70P. The sequence demonstrated 99-100% and 98% similarity to the 18S rRNA gene fragment of B. divergence and Babesia venatorum, respectively. Molecular biological and serological methods of laboratory diagnosis of babesiosis are considered.


Assuntos
Babesia , Babesiose/diagnóstico , Animais , Técnicas de Laboratório Clínico , Primers do DNA , Diagnóstico Diferencial , Eritrócitos/parasitologia , Humanos , Malária Falciparum , Carrapatos/parasitologia
2.
Med Parazitol (Mosk) ; (4): 46-8, 2010.
Artigo em Russo | MEDLINE | ID: mdl-21395044

RESUMO

The efficiency of P. vivax malaria treatment with delagil (chloroquine) was evaluated in 122 patients, including 82 cases in Moscow and the Moscow region. The origin of the cases was malaria endemic areas in Asia, Africa, the Pacific Region, South America, and Transcaucasia. Forty other cases were imported malaria cases (secondary to imported ones), detected in Moscow and the Moscow region. Standard treatment with delagil (2.5 g) resulted in clinical improvement during 3 days in the majority of cases. Initial signs of degradation of asexual stages of P. vivax--kernels of nucleus, refinement of cytoplasm and its vacuolization, aggregation of pigment in isolated instances, its pushing out from cytoplasm--were observed after 1-2 hours after administration of delagil. Thereafter, parasite degradation was increasing, and it disappeared within 48 hours. Disappearance of fever slowed down in a few cases. However, degradation of parasites occurred during the same period among the rest of cases. It can not be excluded that fever was determined by the pyrogenic effect of remnants of degraded parasites and by the products of destroyed infected erythrocytes. It is probable that the findings of gametocytes, not completely degraded after disappearance of asexual forms in conjunction with prolonged fever, could result in a wrong conclusion of drug resistance. Negative results of microscopy and nested PCR on the last day of treatment, as well as in the following 10 days and absence of complains during 45 days, suggest the absence of resistance to delagil in P. vivax strains imported from different regions of the world. It is also probable that the literature on P. vivax resistance to chloroquine is limited to sporadic cases.


Assuntos
Antimaláricos/farmacologia , Cloroquina/análogos & derivados , Malária Vivax/tratamento farmacológico , Plasmodium vivax/efeitos dos fármacos , Antimaláricos/administração & dosagem , Antimaláricos/uso terapêutico , Cloroquina/administração & dosagem , Cloroquina/farmacologia , Cloroquina/uso terapêutico , Resistência a Medicamentos , Humanos , Plasmodium vivax/isolamento & purificação , Federação Russa/epidemiologia , Viagem
3.
Med Parazitol (Mosk) ; (2): 10-2, 2006.
Artigo em Russo | MEDLINE | ID: mdl-16813240

RESUMO

The KAT-Quick P.f. test (KAT Medical, South African Republic) is based on the detection of protein HPR II produced by trophozoites and young gametocytes of P. falciparum. This test was conducted by the authors in the distribution areas of P. falciparum strains differing in the spectrum of drug resistance. Five hundred and forty-nine blood samples from febrile patients in Vietnam (n=84), Sierra Leone (n=41), Nigeria (n=14), Tanzania (n=8), Kenya (n=5), and Tadjikistan (n=397) were tested. Microscopy served as a primary control. Detection of P. falciparum DNA, using polymerase chain reaction (PCR) with included primers (nested PCR) of the most sensitive modification of PCR was a final control. The efficiency of the KAT-Quick P.f. test was estimated as a ratio of the number of its positive results to those of PCR. It was equal to 98-95%. The KAT-Quick P.f. test revealed no false-positive case associated with the genome of the parasite. The specificity of the test was determined as a ratio of the number of its negative (no P. falciparum) results to those of PCR. The blood samples from patients with vivax malaria and from those with nonmalarial fever were investigated. There was no cross reaction of the KAT-Quick P.f. test system for P. falciparum with that for P. vivax. The KAT-Quick P.f. test yielded no positive reaction with the blood from patients with non-malarial fever. Drug resistance depending on the spectrum of specific drugs caused its emergence may be determined by one or several mechanisms that are ultimately determined by one, the key mechanism. Thus, the findings suggest that multidrug resistance of P. falciparum does not trigger the occurrence of changes in its surface antigen--HRPII that is responsible for the efficiency of the KAT-Quick P.f. test. These may be also extrapolated to other rapid tests patterned after the same principle.


Assuntos
Malária Falciparum/diagnóstico , Plasmodium falciparum/isolamento & purificação , Kit de Reagentes para Diagnóstico , África Oriental , África Ocidental , Animais , Antígenos de Protozoários/sangue , Antimaláricos/farmacologia , Resistência a Múltiplos Medicamentos , Humanos , Malária Falciparum/sangue , Plasmodium falciparum/efeitos dos fármacos , Plasmodium falciparum/imunologia , Sensibilidade e Especificidade , Tadjiquistão , Vietnã
4.
Med Parazitol (Mosk) ; (2): 17-20, 2002.
Artigo em Russo | MEDLINE | ID: mdl-12214515

RESUMO

A new rapid KAT Quick Malaria test for the diagnosis of falciparum malaria, which is based on the detection of a monoclonal antibody-antigen complex of malaria parasites, has been worked out by the KAT Medical CC in South Africa. The efficiency and specificity of the KAT test were compared with those of the microscopic method and with the ICT test for rapid diagnosis of P. falciparum and P. vivax. The polymerase chain reaction was used as a control test. Testing for malaria was performed on 98 blood samples from feverish patients in Vietnam and Tadjikistan and among the persons who had returned to Moscow from endemic regions. The efficiency of the KAT test for falciparum-malaria was found to be 100% versus 90.5% with ICT. The absence of cross-reactions with P. vivax and the presence of pseudopositive results of the KAT test for fever cases of non-malaria origin indicate its high specificity. There was no correlation between the rate of test line colouring and the level of parasitemia. The KAT test yielded positive results only when gametocytes were found in blood specimens.


Assuntos
Malária Falciparum/diagnóstico , Plasmodium falciparum/isolamento & purificação , Kit de Reagentes para Diagnóstico , Adolescente , Adulto , Animais , Anticorpos Monoclonais , Complexo Antígeno-Anticorpo/análise , Criança , Histidina , Humanos , Malária Falciparum/sangue , Plasmodium falciparum/imunologia , Proteínas de Protozoários/química , Proteínas de Protozoários/imunologia , Sensibilidade e Especificidade
5.
Mol Biol (Mosk) ; 35(3): 515-25, 2001.
Artigo em Russo | MEDLINE | ID: mdl-11443936

RESUMO

Stages life cycle of the malaria parasite differ in the rate of replication and the structural properties of functionally active A-, S-, and O-type ribosomes. Regions of A-type rDNA including ITS1, 5.8S, and ITS2 from two strains of Plasmodium vivax with different incubation periods were amplified and sequenced. No substantial differences in the sequences of two strains were revealed. Phylogenetic analysis of the obtained and homologous sequences of ITS1 rDNA of A, S, and O types of P. vivax; A and S types of P. falciparum; and Cryptosporidium parvum, Eimeria maxima, Toxoplasma gondii as outgroup, by the maximum parsimony method using PAUP 4.0 revealed that divergence of ITS1 might have occurred after speciation and at different rates in individual lineages of the Plasmodium genus. Basing on the results of the analysis of orthologous sequences of P. vivax and P. falciparum, we developed genus- and species-specific primers for PCR diagnostics of malaria, as well as a one-step effective method of DNA isolation from Giemsa-Romanovsky-stained thick blood smears. It was demonstrated that stained preparations could be a reliable source of plasmodial DNA, and the quality of preparations and storage time (10-20 years) did not interfere with the results of PCR analysis.


Assuntos
DNA Ribossômico/genética , Malária/diagnóstico , Plasmodium vivax/isolamento & purificação , Proteínas de Protozoários/genética , RNA Ribossômico 5,8S/genética , Animais , Sequência de Bases , Primers do DNA , Humanos , Malária/sangue , Dados de Sequência Molecular , Filogenia , Plasmodium vivax/genética , Reação em Cadeia da Polimerase , Homologia de Sequência do Ácido Nucleico
7.
Med Parazitol (Mosk) ; (2): 3-8, 1994.
Artigo em Russo | MEDLINE | ID: mdl-7935186

RESUMO

When Swiss mice were experimentally infected with P. yoelii yoelii 264 BY sporozoites through bites of the mosquito Anopheles stephensi, there were no blood parasites in most (85%) animals after a short (on day 4-5) incubation period. The hepatic parenchymal cells from the animals without primary infection manifestation (following 2 months of the infection) or with the disease self-arrested (on day 26 of postinfection) were the first to display 5-11-microunits mononuclear parasites that are likely to be similar to the hypnozoites of some malaria parasite strains in man and primates. This suggests that rodent malaria parasites, as P. vivax, might cause infection with prolonged incubation and true relapses previously unknown for these parasites. Therefore, P. yoelii is a promising accessible model for studying the persistence phenomenon of malarial parasites at the tissue level.


Assuntos
Plasmodium yoelii/crescimento & desenvolvimento , Animais , Anopheles , Células Cultivadas , Feminino , Insetos Vetores , Fígado/parasitologia , Malária/parasitologia , Malária/transmissão , Camundongos , Plasmodium yoelii/patogenicidade , Recidiva
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...