RESUMO
Radioligand assay showed that the cholinesterase (ChE) reactivators dipiroxime and benzyxime, but not carboxime, modulate selective absorption of some cholinolytics (tributam, pediphen, aprophen) in rat brain. Significant suppression of the specific binding of muscarine antagonists was recorded after chlorophos (2.LD50) intoxication. Under such conditions, the ChE reactivators induce increase in the number of binding sites and in the parameters of the constant of cholinolytic absorption on the brain membranes. It was also established by equilibrium dialysis that the binding of cholinolytics in blood plasma under the effect of ChE reactivators is reduced, leading to redistribution of their free and bound fractures, which is most favorable for tissue sorption.
Assuntos
Encéfalo/metabolismo , Antagonistas Colinérgicos/farmacocinética , Reativadores da Colinesterase/farmacocinética , Plasma/metabolismo , Animais , Química Encefálica , Radioisótopos de Carbono , Antagonistas Colinérgicos/análise , Antagonistas Colinérgicos/uso terapêutico , Reativadores da Colinesterase/análise , Reativadores da Colinesterase/uso terapêutico , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Interações Medicamentosas , Inseticidas/intoxicação , Masculino , Plasma/química , Intoxicação/tratamento farmacológico , Intoxicação/metabolismo , Ensaio Radioligante , Ratos , Fatores de Tempo , Triclorfon/intoxicação , TrítioRESUMO
Conditions for gas-liquid and chromato-mass-spectrometric analyses of chloracetone, dinitril o-chlorobenzylidene malonic acid, and pelargonic acid morpholide have been developed. The proposed methods may be used for identification of these compounds in investigation of material evidence in cases involving the use of defence gas weapons.
Assuntos
Cromatografia Gasosa/métodos , Irritantes/análise , Cromatografia Gasosa/instrumentação , Ácidos Graxos/análise , Cromatografia Gasosa-Espectrometria de Massas/instrumentação , Cromatografia Gasosa-Espectrometria de Massas/métodos , o-Clorobenzilidenomalonitrila/análise , ômega-Cloroacetofenona/análiseRESUMO
Pharmacokinetics of dipyroxime was studied following intravenous injection to noninbred albino male rats. Dipyroxime is a cholinesterase reactivating drug now widely used as an antidote in poisonings caused by organophosphorus pesticides. Dipyroxime pharmacokinetics analysis made it possible to propose a formula for approximation of the data on another animal species (dogs). The drug concentration in the dog blood plasma is in a good correlation with the predicted level.
