RESUMO
The possibility of differentiation of insulin-producing cells and neural and glial elements was demonstrated in the culture of bone marrow stromal cells. The perspectives of use of the bone marrow stromal cells in clinical medicine are considered.
Assuntos
Células da Medula Óssea/fisiologia , Transplante de Células/métodos , Epilepsia/terapia , Células-Tronco Pluripotentes/fisiologia , Células Estromais/fisiologia , Animais , Células da Medula Óssea/citologia , Células da Medula Óssea/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Meios de Cultivo Condicionados/farmacologia , Diabetes Mellitus/terapia , Humanos , Insulina/metabolismo , Células de Langerhans/citologia , Células de Langerhans/efeitos dos fármacos , Células de Langerhans/metabolismo , Camundongos , Neurônios/citologia , Pâncreas/citologia , Células-Tronco Pluripotentes/citologia , Células Estromais/citologia , Células Estromais/efeitos dos fármacos , Tretinoína/farmacologiaRESUMO
The in vitro induced differentiation of mouse bone marrow stromal cells into nerve cells by retinoic acid and leukemia inhibitory factor has been shown, using morphological, histochemical and immunocytochemical analyses. The developed techniques allow to obtain up to 30% of neural cells in vitro. A suggestion about pluripotency of bone marrow stromal cells and possibility of their application to the cell therapy is discussed.
Assuntos
Células da Medula Óssea/citologia , Neurônios/citologia , Células Estromais/citologia , Animais , CamundongosRESUMO
Mutagenic activities of new gel-forming substances proposed for the use in pharmacy and food industry were studied by the method of scoring mutations in test microorganisms. Sodium carboxy-methyl starch and sodium carboxy-methyl-cellulose did not induce gene mutations in Salmonella typhimurium TA 98 and TA 100.