RESUMO
The reference-center of monitoring of agents of glanders and melioidosis carried out testing of reagents kits for diagnostic of agent of melioidosis and other close-related species of Burkholderiae in vitro. At the stage of specific identification of pathogenic Burkholderiae the diagnostic possibilities of commercial and experimental kits of reagents for express- and rapid analysis were evaluated. The criteria of evaluation of diagnostic value of kits of reagents were sensitivity, specificity and time of implementation of studies. The analysis with application of mono- and multi-locus amplification systems, including real-time polymerase chain reaction permitted during 5-6 hours to implement identification and differentiation of Burkholderia pseufomallei, B. thailandensis and B. cepacia.
Assuntos
Burkholderia/isolamento & purificação , Mormo/microbiologia , Melioidose/microbiologia , Reação em Cadeia da Polimerase/métodos , Animais , Técnicas de Tipagem Bacteriana/métodos , Burkholderia/classificação , Burkholderia/genética , Burkholderia/patogenicidade , Mormo/genética , Cavalos/genética , Cavalos/microbiologia , Humanos , Melioidose/diagnóstico , Melioidose/genéticaAssuntos
Anticorpos Monoclonais/biossíntese , Antígenos de Bactérias/imunologia , Burkholderia pseudomallei/imunologia , Hibridomas/imunologia , Ativação Linfocitária/imunologia , Animais , Anticorpos Monoclonais/análise , Especificidade de Anticorpos , Imunização/métodos , Melioidose/imunologia , Camundongos , Camundongos Endogâmicos BALB CRESUMO
The paper summarizes the data concerning the production and study of monoclonal antibodies (MAb) to the diagnostically significant glanders and melioidosis bacillus antigens. It evaluates the efficiency of using MAb in the gel immunodiffusion and agglutination tests as a basis of new-generation preparations for fluorescent antibody assay, indirect hemagglutination test which are used while detecting and identifying pathogenic pseudomonads. The paper defines the quality indices for monoclonal luminescent immunoglobulins and provides evidence for the benefits of monoclonal diagnostic agents over polyclonal analogues.