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1.
Nat Struct Mol Biol ; 31(1): 159-169, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38057552

RESUMO

Sodium-glucose cotransporter 2 (SGLT2) is imporant in glucose reabsorption. SGLT2 inhibitors suppress renal glucose reabsorption, therefore reducing blood glucose levels in patients with type 2 diabetes. We and others have developed several SGLT2 inhibitors starting from phlorizin, a natural product. Using cryo-electron microscopy, we present the structures of human (h)SGLT2-MAP17 complexed with five natural or synthetic inhibitors. The four synthetic inhibitors (including canagliflozin) bind the transporter in the outward conformations, while phlorizin binds it in the inward conformation. The phlorizin-hSGLT2 interaction exhibits biphasic kinetics, suggesting that phlorizin alternately binds to the extracellular and intracellular sides. The Na+-bound outward-facing and unbound inward-open structures of hSGLT2-MAP17 suggest that the MAP17-associated bundle domain functions as a scaffold, with the hash domain rotating around the Na+-binding site. Thus, Na+ binding stabilizes the outward-facing conformation, and its release promotes state transition to inward-open conformation, exhibiting a role of Na+ in symport mechanism. These results provide structural evidence for the Na+-coupled alternating-access mechanism proposed for the transporter family.


Assuntos
Diabetes Mellitus Tipo 2 , Inibidores do Transportador 2 de Sódio-Glicose , Humanos , Inibidores do Transportador 2 de Sódio-Glicose/farmacologia , Transportador 2 de Glucose-Sódio/metabolismo , Proteínas Facilitadoras de Transporte de Glucose , Florizina/farmacologia , Florizina/química , Florizina/metabolismo , Microscopia Crioeletrônica , Glucose/metabolismo
2.
Biochemistry ; 56(3): 458-467, 2017 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-28029774

RESUMO

N1-Acetylspermine oxidase (APAO) catalyzes the conversion of N1-acetylspermine or N1-acetylspermidine to spermidine or putrescine, respectively, with concomitant formation of N-acetyl-3-aminopropanal and hydrogen peroxide. Here we present the structure of murine APAO in its oxidized holo form and in complex with substrate. The structures provide a basis for understanding molecular details of substrate interaction in vertebrate APAO, highlighting a key role for an asparagine residue in coordinating the N1-acetyl group of the substrate. We applied computational methods to the crystal structures to rationalize previous observations with regard to the substrate charge state. The analysis suggests that APAO features an active site ideally suited for binding of charged polyamines. We also reveal the structure of APAO in complex with the irreversible inhibitor MDL72527. In addition to the covalent adduct, a second MDL72527 molecule is bound in the active site. Binding of MDL72527 is accompanied by altered conformations in the APAO backbone. On the basis of structures of APAO, we discuss the potential for development of specific inhibitors.


Assuntos
Oxirredutases/química , Putrescina/química , Espermidina/análogos & derivados , Espermidina/química , Espermina/análogos & derivados , Aldeídos/química , Aldeídos/metabolismo , Animais , Domínio Catalítico , Expressão Gênica , Peróxido de Hidrogênio/química , Peróxido de Hidrogênio/metabolismo , Cinética , Camundongos , Modelos Moleculares , Oxirredução , Oxirredutases/genética , Oxirredutases/metabolismo , Propilaminas/química , Propilaminas/metabolismo , Estrutura Secundária de Proteína , Putrescina/análogos & derivados , Putrescina/metabolismo , Espermidina/metabolismo , Espermina/química , Espermina/metabolismo
3.
PLoS One ; 10(10): e0140366, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26460611

RESUMO

The Skp1-Cul1-F-box protein (SCF) complex catalyzes protein ubiquitination in diverse cellular processes and is one of the best-characterized ubiquitin ligases. F-box proteins determine the substrate specificities of SCF ubiquitin ligases. Among these, Fbs1/FBG1/FBXO2, Fbs2/FBG2/FBXO6, and Fbs3/FBG5/FBXO27 recognize the N-glycans of glycoproteins, whereas FBG3/FBXO44 has no sugar-binding activity, despite the high sequence homology and conservation of the residues necessary for oligosaccharide binding between Fbs1-3 and FBG3. Here we determined the crystal structure of the Skp1-FBG3 complex at a resolution of 2.6 Å. The substrate-binding domain of FBG3 is composed of a 10-stranded antiparallel ß-sandwich with three helices. Although the overall structure of FBG3 is similar to that of Fbs1, the residues that form the Fbs1 carbohydrate-binding pocket failed to be superposed with the corresponding residues of FBG3. Structure-based mutational analysis shows that distinct hydrogen bond networks of four FBG3 loops, i.e., ß2-ß3, ß5-ß6, ß7-ß8, and ß9-ß10, prevent the formation of the carbohydrate-binding pocket shown in Fbs1.


Assuntos
Proteínas de Ciclo Celular/química , Proteínas F-Box/química , Glicoproteínas/metabolismo , Proteínas do Tecido Nervoso/química , Sequência de Aminoácidos , Cristalografia por Raios X , Proteínas F-Box/genética , Humanos , Modelos Moleculares , Dados de Sequência Molecular , Proteínas Mutantes/metabolismo , Mutação/genética , Ligação Proteica , Estrutura Terciária de Proteína , Ribonucleases/metabolismo , Proteínas Quinases Associadas a Fase S/química , Homologia de Sequência de Aminoácidos , Especificidade por Substrato
4.
Artigo em Inglês | MEDLINE | ID: mdl-20057081

RESUMO

F-box proteins are the substrate-recognition components of Skp1-Cullin1-F-box protein-Rbx1 (SCF) ubiquitin ligase complexes. Fbs1, an F-box protein, binds specifically to proteins modified with high-mannose oligosaccharides. Fbg3, another F-box protein, has 51% sequence identity to Fbs1. Although the residues that are necessary for binding to oligosaccharides are conserved between Fbs1 and Fbg3, Fbg3 does not bind glycoproteins. Skp1 and Fbg3 were co-expressed in Escherichia coli and their complex was purified to homogeneity and crystallized. Microseeding combined with the sandwiched hanging-drop technique improved the quality of the resulting crystals. The plate-shaped crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.1, b = 76.6, c = 193.9 A and one molecule per asymmetric unit.


Assuntos
Proteínas F-Box/química , Proteínas Ligases SKP Culina F-Box/química , Cristalização , Cristalografia por Raios X , Humanos
5.
J Biol Chem ; 283(33): 22847-57, 2008 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-18524774

RESUMO

Impairment of autophagic degradation of the ubiquitin- and LC3-binding protein "p62" leads to the formation of cytoplasmic inclusion bodies. However, little is known about the sorting mechanism of p62 to autophagic degradation. Here we identified a motif of murine p62 consisting of 11 amino acids (Ser334-Ser344) containing conserved acidic and hydrophobic residues across species, as an LC3 recognition sequence (LRS). The crystal structure of the LC3-LRS complex at 1.56 angstroms resolution revealed interaction of Trp340 and Leu343 of p62 with different hydrophobic pockets on the ubiquitin fold of LC3. In vivo analyses demonstrated that p62 mutants lacking LC3 binding ability accumulated without entrapping into autophagosomes in the cytoplasm and subsequently formed ubiquitin-positive inclusion bodies as in autophagy-deficient cells. These results demonstrate that the intracellular level of p62 is tightly regulated by autophagy through the direct interaction of LC3 with p62 and reveal that selective turnover of p62 via autophagy controls inclusion body formation.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/fisiologia , Autofagia/fisiologia , Proteínas Adaptadoras de Transdução de Sinal/química , Proteínas Adaptadoras de Transdução de Sinal/genética , Sítios de Ligação , Proteínas de Transporte/química , Proteínas de Transporte/genética , Lisossomos/fisiologia , Proteínas Ligantes de Maltose , Proteínas Associadas aos Microtúbulos/química , Proteínas Associadas aos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/fisiologia , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/metabolismo , Deleção de Sequência , Proteína Sequestossoma-1 , Vacúolos/fisiologia
6.
Proc Natl Acad Sci U S A ; 104(14): 5777-81, 2007 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-17389369

RESUMO

The ubiquitin ligase complex SCF(Fbs1), which contributes to the ubiquitination of glycoproteins, is involved in the endoplasmic reticulum-associated degradation pathway. In SCF ubiquitin ligases, a diverse array of F-box proteins confers substrate specificity. Fbs1/Fbx2, a member of the F-box protein family, recognizes high-mannose oligosaccharides. To elucidate the structural basis of SCF(Fbs1) function, we determined the crystal structures of the Skp1-Fbs1 complex and the sugar-binding domain (SBD) of the Fbs1-glycoprotein complex. The mechanistic model indicated by the structures appears to be well conserved among the SCF ubiquitin ligases. The structure of the SBD-glycoprotein complex indicates that the SBD primarily recognizes Man(3)GlcNAc(2), thereby explaining the broad activity of the enzyme against various glycoproteins. Comparison of two crystal structures of the Skp1-Fbs1 complex revealed the relative motion of a linker segment between the F-box and the SBD domains, which might underlie the ability of the complex to recognize different acceptor lysine residues for ubiquitination.


Assuntos
Proteínas Ligases SKP Culina F-Box/química , Proteínas Ligases SKP Culina F-Box/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação , Configuração de Carboidratos , Sequência de Carboidratos , Cristalografia por Raios X , Retículo Endoplasmático/enzimologia , Escherichia coli/genética , Glicoproteínas/metabolismo , Glicosilação , Humanos , Ligação de Hidrogênio , Manose/química , Modelos Químicos , Modelos Moleculares , Dados de Sequência Molecular , Oligossacarídeos/química , Ligação Proteica , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Ribonucleases/química , Ribonucleases/metabolismo , Proteínas Ligases SKP Culina F-Box/genética , Proteínas Ligases SKP Culina F-Box/isolamento & purificação , Eletricidade Estática , Especificidade por Substrato
7.
J Mol Biol ; 355(4): 612-8, 2006 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-16325851

RESUMO

Autophagy is an evolutionarily conserved pathway in which the cytoplasm and organelles are engulfed within double-membrane vesicles, termed autophagosomes, for the turnover and recycling of these cellular constituents. The yeast Atg8 and its human orthologs, such as LC3 and GABARAP, have a unique feature as they conjugate covalently to phospholipids, differing from ubiquitin and other ubiquitin-like modifiers that attach only to protein substrates. The lipidated Atg8 and LC3 localize to autophagosomal membranes and play indispensable roles for maturation of autophagosomes. Upon completion of autophagosome formation, some populations of lipidated Atg8 and LC3 are delipidated for recycling. Atg4b, a specific protease for LC3 and GABARAP, catalyzes the processing reaction of LC3 and GABARAP precursors to mature forms and de-conjugating reaction of the modifiers from phospholipids. Atg4b is a unique enzyme whose primary structure differs from that of any other proteases that function as processing and/or de-conjugating enzymes of ubiquitin and ubiquitin-like modifiers. However, the tertiary structures of the substrates considerably resemble that of ubiquitin except for the N-terminal additional domain. Here we determined the crystal structure of human Atg4b by X-ray crystallography at 2.0 A resolution, and show that Atg4b is a cysteine protease whose active catalytic triad site consists of Cys74, His280 and Asp278. The structure is comprised of a left lobe and a small right lobe, designated the "protease domain" and the "auxiliary domain", respectively. Whereas the protease domain structure of Atg4b matches that of papain superfamily cysteine proteinases, the auxiliary domain contains a unique structure with yet-unknown function. We propose that the R229 and W142 residues in Atg4b are specifically essential for recognition of substrates and catalysis of both precursor processing and de-conjugation of phospholipids.


Assuntos
Autofagia , Cisteína Endopeptidases/química , Cisteína Endopeptidases/metabolismo , Precursores de Proteínas/química , Precursores de Proteínas/metabolismo , Proteínas Relacionadas à Autofagia , Sítios de Ligação , Catálise , Cristalografia por Raios X , Humanos , Modelos Moleculares , Ligação Proteica , Processamento de Proteína Pós-Traducional , Estrutura Terciária de Proteína , Homologia Estrutural de Proteína , Especificidade por Substrato
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