Regulation of fungal communities during pig manure composting.

The role of protein shell (PS) amendment in altering the fungal community during pig manure (PM) composting was investigated. Six different dosages of PS based on the dry weight of PM (0 %, 2.5 %, 5 %, 7.5 %, 10 %, and 12 %; T1-T6, respectively) were mixed with wheat straw to make the initial feedstock and composted for 42 days. The results showed that Ascomycota, Basidiomycota, and Giomeromycota were the most abundant phyla in all treatments. However, the relative abundance of Giomeromycota was the highest in the control treatment, although a substantially greater population was observed in all treatments. Genus abundance declined steadily from T1 to T6; however, T4 and T6 had smaller populations. Correlation analysis also suggested that T6 amendment increased the overall fungal dynamics and organic matter degradation. Thus, T6 was more efficient to enhance the overall fungal population and dynamics with considerable network connections among all the analyzed parameters.

Biochar as smart organic catalyst to regulate bacterial dynamics during food waste composting.

The impact of wheat straw biochar (WSB) on bacterial dynamics succession during food waste (FW) composting was analyzed. Six treatments [0(T1), 2.5(T2), 5 (T3), 7.5 (T4), 10 (T5), and 15 %(T6)] dry weight WSB were used with FW and saw dust for composting. At the highest thermal peak at 59 ℃ in T6, the pH varied from 4.5 to 7.3, and electrical conductivity among the treatments varied from 1.2 to 2.0 mScm1. Firmicutes (25-97 %), Proteobacteria (8-45 %), and Bacteroidota (5-50 %) were among the dominate phyla of the treatments. Whereas, Bacillus (5-85 %), Limoslactobacillus (2-40 %), and Sphingobacterium (2-32 %) were highest among the identified genus in treatments but surprisingly Bacteroides was in greater abundance in the control treatments. Moreover, heatmap constructed with 35 various genera in all the treatments showed that Gammaproteobacterial genera contributed in large proportion after 42 days in T6. Additionally, a dynamic shift from Lactobacillus fermentum to higher abundance of Bacillus thermoamylovorans was reported on 42 days of FW composting. Biochar 15 % amendment can improve FW composting by influencing bacterial dynamics.

Effect of scleral protein shell amendment on bacterial community succession during the pig manure composting.

The impact of scleral protein shell (SPS) amendment on bacterial community succession during pig manure (PM) composting were evaluated in the present work. Five treatments representing different dry weight dosage of SPS [0 % (T1), 2.5 % (T2), 5 % (T3), 7.5 % (T4), 10 % (T5) and 12 % (T6)] were applied with initial mixture of raw materials (Wheat straw along with the PM) and composted for 42 days. Results indicated that the dominant of phyla were Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes. The relative abundance (RA) of genus un-identified, Ruminofilibacter, Thermovum, Longispora and Pseudomonas were greater among the all treatments but interestingly genus Ruminofilibacter was also higher in control treatment. The network analysis was confirmed that T6 treatment with higher dosage of SPS amendment could enhance the bacterial population and rate of organic matter mineralization. Compared with T1, the T5 has greater potential impact to enhance the bacterial population and significant correlation among the pH and temperature.

Changes of fungal diversity in fine coal gasification slag amendment pig manure composting.

The purpose of this study was to investigate fungal diversity and relative abundance (RA) during pig manure composting via high-throughput sequencing approach. Fine coal gasification slag (FCGS) (0%, 2%, 4%, 6%, 8% and 10%) were added into composting raw materials as additive and performed 42 days. Adjust C/N and moisture to 30 and 65%. Results showed that dominant phyla were Ascomycota (99.62%) and Basidiomycota (0.38%). The main genera were Epicoccum (1.26%), Alternaria (83.35%), Aspergillus (12.08%) and Gibberella (1.69%). 10% treatment got the higher abundance and operational taxonomic units number from rank abundance curve and petals diagram. Compared with control, FCGS amendment composting could increase the sanitary time (3-7 d) and total nitrogen (0.05-12.03%). The principal component analysis was considered that FCGS treatments and control had significantly difference. The RA of fungi varied among all treatments. Therefore, 10% treatment was a potential candidate to enhance fungal diversity and composting quality.

Influence of fine coal gasification slag on greenhouse gases emission and volatile fatty acids during pig manure composting.

This study was evaluated industrial waste fine coal gasification slag (FCGS) as an additive on pig manure composting by parameters of greenhouse gases, NH3, volatile fatty acids (VFAs) and maturity. Six treatments of FCGS (0%, 2%, 4%, 6%, 8% and 10%) were added into the mixture raw material and composted 42 days. Results illustrated that the FCGS amendment could prolong sanitation stage and promote the degree of maturity, germination index and C/N ratio during composting. With the increasing amendment of FCGS, GI was increased from 9.97 to 28.45%. Compared with control, increasing of FCGS proportion could reduce the mitigation of global warming potential (N2O and CH4), NH3 and cumulative of VFAs from 8.89-77.04%, 3.81-71.65%, 5.18-28.02% and 8.79-83.33%. Finally, present study results revealed that 10%FCGS could improve composting and reduced the maturity period as well as compost quality, thus recommended as effective dosage for efficient pig manure composting.

Microbial dynamics for lignocellulosic waste bioconversion and its importance with modern circular economy, challenges and future perspectives.

An extensive use of microbial dynamics for utilizing the lignocellulosic wastes has been attributed to their efficiency in bioenergy and bioproducts development as a cost effective high nutritional value. The integration of lignocellulosic waste into the circular economy can scaleup the sustainable bioproducts and bioenergy development. In this review paper, the aim is to describe the existing research efforts on organic lignocellulosic waste, cellulase producing microbes, their potential enzyme, modern circular economy with associated challenges and future perspectives. Presently, it has been reviewed that microbial cellulases have provided treasure bioproducts visions into industrial bioproducts marvels unveiled through lignocellulosic waste cutting-edge microbial explorations. Furthermore, the review focused on new insights of the growing circular economy of lignocellulosic waste used for many bioproducts and bioenergy dealings and explored the emergent lignocellulosic biorefinery approaches which could then be applied to review industrial-scale sustainable economic models for upgraded bioproducts and other production associated problems.

Effects of clay on nitrogen cycle related functional genes abundance during chicken manure composting.

The objective of this study was to identify the effect of clay additive to explore the nitrogen cycling related ammonification, denitrifying genes and their relation N2O and ammonia emission during the chicken manure (CM) composting. The consequences indicated that the additive clay could optimize the environment and improve the NH4+-N into NO3--N transformation. Meanwhile, the results observed that 8% clay amendment was significant correlated the abundance of nifH and nosZ genes. Compared with the other treatments, the 6% and 10% clay treatments observed low abundance of nifH. The heat map indicated that clay amendment could enhance the function genes abundance. The redundancy analysis showed that N2O and ammonia emission among all physiochemical parameters had great relationship with functional genes. Therefore, the addition of 10% clay into CM composting system could efficiently enhance the abundance of nirK and narG genes, and improve the composting with its application.

An assessment of the persistence of pathogenic bacteria removal in chicken manure compost employing clay as additive via meta-genomic analysis.

The aim was to evaluate pathogenic bacteria (PB) survival during the composting of chicken manure (CM) amended with five different dosages of clay compared to CM without clay-applied treatment. The results showed that 85-87% of PB relative abundances (RAs) were significantly reduced in lower dosages of applied clay (T2 and T3). However, the maximum survival of PB was noticed in the T6 and T5 treatments, but most of the PB belong to Firmicutes, Actinobacteria and Proteobacteria phylum and their derivative bacterial species. The changes in PB during the composting were not only strongly influenced by clay amendment but also significantly associated with the succession of bacterial species in compost. Bacillus, Clostridium, Mycobacterium and Klebsiella were the dominant spore-forming bacteria identified in higher dosages of clay (i.e., T4, T5 and T6) treatments, but very low abundance of these bacterial genus and its species were recovered from lower dosages of clay (T2 and T3)-applied treatments. Overall, without clay, amended-CM-derived compost contained a relatively higher PB abundance than other treatments, as the anaerobic bacterial species Clostridium_difficile_AA1, Vibrio_cholerae, and Acinetobacter_calcoaceticus had relatively greater RAs, followed by Klebsiella_oxytoca_10-5248, Paenibacillus_Bacillus_cereus and Bartonella_quintana_RM-11. Thus, CM composting with 4% clay amendment is considered a useful method for the efficient recycling of CM, as this process produced sanitized compost with less survival of PB.

Heterogeneity of zeolite combined with biochar properties as a function of sewage sludge composting and production of nutrient-rich compost.

In the present study, biochar combined with a higher dosage of zeolite (Z) and biochar (B) alone were applied as additives for dewatered fresh sewage sludge (DFSS) composting using 130-L working volume lab-scale reactors. We first observed that the addition of a mixture of B and Z to DFSS equivalent to 12%B+10% (Z-1), 15% (Z-2) and 30% (Z-3) zeolite (dry weight basis) worked synergistically as an amendment and increased the composting efficiency compared with a treatment of 12%B alone amended and a control without any amendment. In a composting reactor, the addition of B+Z may serve as a novel approach for improving DFSS composting and the quality of the end product in terms of the temperature, water-holding capacity, CO2 emissions, electrical conductivity, water-soluble and total macro-nutrient content and phytotoxicity. The results indicated that during the thermophilic phase, dissolved organic carbon, NH4+-N and NO3--N increased drastically in all biochar amended treatments, whereas considerably low water-soluble nutrients were observed in the control treatment throughout and at the end of the composting. Furthermore, the maturity parameters and dissolved organic carbon (DOC) indicated that compost with 12%B+15%Z became more mature and humified within 35days of DFSS composting, with the maturity parameters, such as CO2 evolution and the concentration of NH4+-N in the compost, being within the permissible limits of organic farming in contrast to the control. Furthermore, at the end of composting, the addition of higher dosage of biochar (12%) alone and 12% B+Z lowered the pH by 7.15 to 7.86 and the electrical conductivity by 2.65 to 2.95mScm-1 as compared to the control, while increased the concentrations of water-soluble nutrients (gkg-1) including available phosphorus, sodium and potassium. In addition, greenhouse experiments demonstrated that the treatment of 150kgha-1 biochar combined with zeolite and that of 12%B alone improved the yield of Chinese cabbage (Brassica rapa chinensis L.). The highest dry weight biomass (1.41±0.12g/pot) was obtained with 12%B+15%Z amended compost. Therefore, 12%B+15%Z can be potentially applied as an amendment to improve DFSS composting.