RESUMO
In this report, polysaccharides - alginate, fucoidan, laminaran - were isolated from marine algae Saccharina cichorioides and Fucus evanescens and their activity as a reducing and stabilizing agents in the biogenic synthesis of silver nanoparticles was evaluated. The cytotoxic and antibacterial properties of obtained nanoparticles were also assessed. It was found that all tested polysaccharides could be used as a reducing agent; however, their catalytic activities varied significantly in the following range alginate < fucoidan < laminaran. Nanoparticles demonstrated cytotoxicity against rat C6 glioma cells. It was considerably higher for alginate- and laminaran-obtained nanosilver samples compared to fucoidan. Additionally, silver nanoparticles possessed considerable antibacterial properties more pronounced in fucoidan-obtained samples. Our data demonstrate that different algal polysaccharides can be used for the synthesis of silver nanoparticles with varying bioactivities.
Assuntos
Alginatos/química , Antibacterianos/química , Excipientes/química , Glucanos/química , Nanopartículas Metálicas/química , Phaeophyceae/química , Polissacarídeos/química , Substâncias Redutoras/química , Prata/química , Agrobacterium tumefaciens/efeitos dos fármacos , Alginatos/isolamento & purificação , Animais , Antibacterianos/farmacologia , Catálise , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Glucanos/isolamento & purificação , Polissacarídeos/isolamento & purificação , Ratos , Alga Marinha/químicaRESUMO
This study addresses the potentialities of flow cytometry in analyzing the composition of circulating hemocyte populations in the ascidian Halocynthia aurantium (Pallas, 1787) both using monoclonal antibodies (mAbs) against some human leukocyte conservative adhesion molecules and without mAbs. Flow cytometry, based on the assessment of forward and side scattering revealed five hemocyte populations. From the wide panel of antibodies against human leukocyte adhesion molecules (CD15, CD29, CD34, CD54, CD62L, CD62P, CD90, CD94, CD117, CD 166), only two mAbs (against CD54, CD90) displayed cross-reactivity with the H. aurantium hemocyte surface antigens. Distribution patterns of these antigens across the hemocyte populations have been analyzed.
Assuntos
Citometria de Fluxo/métodos , Hemócitos/citologia , Urocordados/citologia , AnimaisRESUMO
The current paper is devoted to the investigation of ascidian circulating hemocytes populations which are used as an example of blood cell specialization in the lower Chordates. Work has been performed on a solitary ascidian Halocynthis aurantium from the Japanese Sea. Using light microscopy and histiochemistry, we have identified five main populations of circulating hemocytes (hemoblasts, granulocytes, hyaline amoebocytes, macrophage-like and morula cells), which are typical of all tunicates. Pigment cells were not revealed. The possible pathway of circulating cells differentiation is assumed.
