RESUMO
BACKGROUND/OBJECTIVES: Replication initiator 1 (Repin1) gene encodes for a zinc-finger protein and has been implicated in the regulation of adipocyte cell size and glucose transport in vitro. Here, we investigate the consequences of reduced adipose tissue (AT) Repin1 expression in vivo. SUBJECTS/METHODS: We have inactivated the Repin1 gene in adipose tissue (iARep-/-) at an age of 4 weeks using tamoxifen-inducible gene targeting strategies on the background of C57BL/6NTac mice. Furthermore, we differentiated human primary adipocytes derived from subcutaneous AT in vitro and knocked down REPIN1 using siRNA technique to measure glycerol release. RESULTS: Conditional Repin1 inactivation results in decreased AT mass, smaller adipocytes in both, subcutaneous and epigonadal AT compared to controls. Compared to controls, iARep-/- mice were more insulin sensitive, had better glucose tolerance and lower LDL-, HDL- and total cholesterol. Significantly lower AT expression of the Repin1 target genes Cd36 and Lcn2 may contribute to the phenotype of iARep-/- mice. Knockdown of REPIN1 in human in vitro differentiated adipocytes revealed an increased glycerol release. CONCLUSIONS: In conclusion, deficiency of Repin1 in AT causes alterations in AT morphology and function, which may underlay lower body weight and improved parameters of insulin sensitivity, glucose and lipid metabolism.
Assuntos
Tecido Adiposo/metabolismo , Proteínas de Ligação a DNA/deficiência , Resistência à Insulina/fisiologia , Metabolismo dos Lipídeos/fisiologia , Fígado/metabolismo , Animais , Proteínas de Ligação a DNA/genética , Modelos Animais de Doenças , Regulação da Expressão Gênica , Técnicas de Silenciamento de Genes , Resistência à Insulina/genética , Metabolismo dos Lipídeos/genética , Camundongos , Camundongos Knockout , Proteínas de Ligação a RNARESUMO
We describe 2 patients with a Sjögren-like syndrome apparently secondary to hypertriglyceridemia. Both had bilateral parotitis in addition to musculoskeletal and sicca symptoms. Parotid gland histology revealed fatty infiltration with no inflammation. Therapy with dietary modification and triglyceride lowering drugs resulted in resolution of symptoms and parotid swelling in one patient. In the 2nd patient, hypertriglyceridemia was resistant to triglyceride lowering drugs, and parotid symptoms and swelling continued unabated. Our findings suggest aggressive treatment of hyperlipidemia in pseudo-Sjögren's syndrome may result in amelioration of musculoskeletal and parotid symptoms.
Assuntos
Hipertrigliceridemia/complicações , Glândula Parótida/patologia , Síndrome de Sjogren/etiologia , Síndrome de Sjogren/patologia , Adulto , Feminino , Humanos , Síndrome de Sjogren/sangueRESUMO
Four novel triterpenoid saponins were isolated from the underground parts of Bellis perennis. The structures were elucidated as 3-O-beta-D-glucopyranosides of 2 beta,3 beta,16 alpha-trihydroxyolean-12-ene-28-oic acid-28-alpha-L- rhamnopyranosyl(1----2)-[beta-D-glucopyranosyl(1----6)]-beta-D- glucopyranoside, 2 beta,3 beta,23-trihydroxyolean-12-ene-28-oic acid-28-O-beta-D-xylopyranosyl (1----2)-[beta-D-glucopyranosyl (1----6)]- beta-D-glucopyranoside and 2 beta,3 beta,23-trihydroxyolean-12-ene-28-oic acid-28-O-alpha-L-rhamnopyranosyl(1----2)-[beta-D-glucopyranosyl(1----6) ]- beta-D-glucopyranoside and as 3-O-alpha-L-rhamnopyranosyl-2 beta,3 beta,23-trihydroxyolean-12-ene-28-oic acid-28-O-beta-D-glucopyranosyl(1----2)-[beta-D-glucopyranosyl(1----6)]- beta-D-glucopyranoside by means of high field 1D and 2D NMR spectroscopic methods without recourse to derivatization or comparison with previous data.
Assuntos
Plantas/química , Saponinas/química , Triterpenos/química , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Estrutura MolecularRESUMO
A 34-year-old man with diffuse eosinophilic fasciitis and a hypocellular myelodysplastic syndrome underwent marrow transplantation from an HLA-identical brother. Prompt hematopoietic reconstitution was observed, strongly suggesting that the marrow hypocellularity was caused by neither a serum inhibitory factor nor a microenvironmental disorder. The patient died of disseminated cytomegalovirus infection too early to evaluate the impact of hematopoietic reconstitution on the eosinophilic fasciitis. Nevertheless, marrow transplantation may offer a therapeutic option for those patients with this disorder who develop severe hematopoietic dysfunction and who have a suitable marrow donor.
Assuntos
Transplante de Medula Óssea , Eosinofilia/complicações , Fasciite/complicações , Síndromes Mielodisplásicas/complicações , Adulto , Eosinofilia/patologia , Eosinofilia/cirurgia , Família , Fasciite/patologia , Fasciite/cirurgia , Antígenos HLA/análise , Humanos , Masculino , Doadores de TecidosRESUMO
We have described a case of sudden blindness of the right eye in an 81-year-old white woman with a one-month history of increasingly severe jaw claudication and a normal Westergren sedimentation rate. Isolated jaw claudication in the elderly is of diagnostic importance, and the need for temporal artery biopsy and rapid institution of high-dose steroid therapy to prevent blindness is emphasized.
Assuntos
Arterite de Células Gigantes/diagnóstico , Claudicação Intermitente/etiologia , Arcada Osseodentária , Idoso , Cegueira/etiologia , Cegueira/prevenção & controle , Sedimentação Sanguínea , Feminino , Arterite de Células Gigantes/sangue , Arterite de Células Gigantes/patologia , Humanos , Dor/diagnóstico , Artérias Temporais/patologiaRESUMO
Venous angiomas are the rarest of all CNS vascular malformations. The association of a sublingual venous angioma with a similar ipsilateral cerebral lesion in an otherwise normal patient was observed.