A Case Report on Myotendinous Junction.

Myotendinous junction is the transition zone between the muscle and its tendon. Hence, it is subject to immense stress within the muscle. In this study, it is found that muscles having a greater tensile have a more arranged myotendinous junction compared to muscles with lesser tensile strength. Cadaveric specimens - plantaris, gastrocnemius, and soleus have been histologically studied to study the same.

Cryptosporidiosis in Yellow Nail Syndrome - A Rare Case Report.

BACKGROUND: Cryptosporidium species infection causes malabsorption and severe diarrhea in immunocompromised hosts. Association of Yellow Nail Syndrome (YNS) and Cryptosporidiosis is rare and has not been reported till date. Immunity can also be affected in this case of YNS is associated with autoimmune disorders. CASE PRESENTATION: Here, we describe a case of persistent diarrhea in an 8 month old YNS patient. Modified Ziehl-Neelsen staining and Saffranine-Methylene blue revealed oocyts of Cryptosporidium species. Following appropriate treatment, the patient's symptoms improved and the patient was discharged in a hemodynamically stable condition. DISCUSSION: Cryptosporidiosis is a significant cause of morbidity and mortality in immunocompromised patients. YNS per se as well as treatment including steroids leads to immunosuppression in individuals making them susceptible host for opportunistic infections like Cryptosporidiosis. CONCLUSION: Clinicians should be aware of the condition and screen for Cryptosporidiosis in any immunocompromised patients with diarrheal symptoms, as parasitic infection like this are opportunistic in them.

Detection of Antibodies against Toxoplasma from Human Serum Sample using ELISA.

BACKGROUND: Toxoplasmosis is a common worldwide zoonotic infection affecting warm blooded animals and humans caused by Toxoplasma gondii. Clinical features range from mildfebrile illness and lymphadenopathy in the immunocompetent host to encephalitisin the immunosuppressed host (E.g. HIV infected patients). Congenital infection can also occur. For effective control and treatment of toxoplasmosis, accurate detection of T. gondii infection is important. OBJECTIVE: In this study, ELISA detecting anti-toxoplasma antibodies IgM and IgG has been used for diagnosing Toxoplasmosis in patients. METHODS: Enzyme-linked immunosorbentassay (ELISA) was carried out in serum samples collected from 40 patients to detect anti-toxoplasma IgG and IgM antibodies as a part of work up in suspected cases. Relevant clinical history was also taken. RESULTS: Of the total 40 samples taken, only one sample came positive for IgM and 9 came positive for IgG antibody. All patients who were seropositivefor T. gondii antibodies had HIV infection. Five patients were in the age group between 30 to 40 years. CONCLUSION: From the limited data available in this study, it may be recommended to screen for T. gondii antibodies in HIV patients.

A Case of Toxoplasma gondii and Strongyloides stercoralis Coinfection in an Immunocompromised Patient.

BACKGROUND: In India, around 23.49 lac people have HIV/AIDS (PLHA). Strongyloidiasis and toxoplasmosis are some of the opportunistic infections that occur in HIV patients, but their dual coinfection with HIV is rarely reported. CASE PRESENTATION: A 46-year-old male was admitted to the hospital with generalized weakness, loss of weight, and bleeding per rectum for the last 3 months and a few episodes of dizziness and fever. RESULTS: On routine investigation, he was diagnosed with human immunodeficiency virus (HIV) infection. On further evaluation, Toxoplasma gondii and Strongyloides stercoralis coinfection, as assessed by the parasitological diagnosis of the serum sample, was positive. Patient was started on artesunate, ART regimen (Tab, TLD- Dolutegravir 50 + Lamivudine 300 + Tenofovir DF 300) and cotrimoxazole. CONCLUSION: Herein, we report a case of Toxoplasma gondii and Strongyloides stercoralis coinfection in an immunocompromised patient.