RESUMO
Expression of heparanase-1 in prostate tumors was evaluated by RT-PCR, immunoblotting, and immunohistochemistry. Malignant transformation was shown to be associated with considerable increase in the expression of heparanase-1 at both mRNA and protein levels, which correlated with the degree of metastasizing and can be used as the marker for diagnostics of the metastatic process.
Assuntos
Regulação Enzimológica da Expressão Gênica/fisiologia , Regulação Neoplásica da Expressão Gênica/fisiologia , Glucuronidase/metabolismo , Metástase Neoplásica/diagnóstico , Neoplasias da Próstata/metabolismo , Primers do DNA/genética , Humanos , Immunoblotting/métodos , Imuno-Histoquímica/métodos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodosRESUMO
Structural modification of the urothelium was studied in various diseases of the urinary bladder and prostate, including urinary bladder cancer, vibration cystopathy, chronic prostatitis, benign prostate hyperplasia, and chronic cystitis. The general phenomena of changes in urinary bladder epithelium were atrophy, squamous metaplasia, and instability of the urothelium (focal atrophy, dysplasia, hyperplasia, and metaplasia). This phenomenon can be interpreted as a morphological marker for cancer risk.
Assuntos
Doenças da Bexiga Urinária/patologia , Urotélio/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Atrofia/patologia , Cistite/patologia , Feminino , Humanos , Masculino , Metaplasia/patologia , Pessoa de Meia-Idade , Próstata/patologia , Doenças Prostáticas/patologia , Hiperplasia Prostática/patologia , Prostatite/patologia , Bexiga Urinária/patologia , Neoplasias da Bexiga Urinária/patologia , Adulto JovemRESUMO
Operation material from patients with various forms of urinary bladder cancer was examined. Systemic involvement resulting from multistage and multifocal tumor growth due to previous multicentric changes was demonstrated. Fluorescent study showed that in urinary bladder cancer tumor transformation involves not only adjacent, but also distant mucosa.
Assuntos
Neoplasias da Bexiga Urinária/patologia , Apoptose , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/ultraestrutura , Carcinoma de Células de Transição/patologia , Carcinoma de Células de Transição/ultraestrutura , Diferenciação Celular , Humanos , Medições Luminescentes , Microscopia Eletrônica , Mucosa/patologia , Mucosa/ultraestrutura , Neoplasias da Bexiga Urinária/ultraestruturaRESUMO
A new form of morphogenesis of pathological process, cystopathy, was distinguished on the basis endoscopy data and morphofunctional analysis of the urinary bladder in chronic cystitis. Cystopathy is characterized by predominance of diffuse degeneration and atrophy of the urothelium, stromal sclerosis, absence of inflammatory cell infiltration, and inhibition of biosynthetic reactions in urothelial cells (compared to chronic cystitis). Cystopathy results from regeneratory and plastic failure. Instability of the bladder epithelium can be a morphological marker of oncological risk.
