RESUMO
The composition of the headspace gas affected the growth dynamics of microbial populations and the biotransformation pattern of p-toluic acid in anoxic estuarine sediments. Under CO2 atmosphere, p-toluic acid was transformed by the sediment microorganisms without a lag period, while under N2/H2 atmosphere, p-toluic acid was transformed after a lag period of 55 days. Under the N2/H2 atmosphere, the methanogen population, following a rapid increase of almost two orders of magnitude, remained at a high level until just before the onset of biotransformation. We hypothesize that during the lag period, the hydrogenotrophic methanogens were removing the H2, a step which is essential before the reaction can be exergonic. Acetogenic bacteria did not initiate decarboxylation as the first step of biotransformation under either atmosphere. Neither the methanogens nor the acetogenic bacteria appeared to be directly involved in the biotransformation of p-toluic acid under either atmosphere. Under the CO2 atmosphere, biotransformation of p-toluic acid involved sulfate-reducing bacteria, while under N2/H2, both sulfate-reducing bacteria and other eubacteria were involved.
Assuntos
Benzoatos/metabolismo , Poluentes Químicos da Água/metabolismo , Bactérias , Biotransformação , Dióxido de Carbono/química , Sedimentos Geológicos , Hipóxia , Nitrogênio/química , Dinâmica PopulacionalRESUMO
The potential for anaerobic biodegradation of 1,1,1-trichloro-2,2-bischlorophenylethane (DDT), 1,1-dichloro-2,2,-bischlorophenylethane (DDD), and dichlorodiphenylchloroethylene (DDE) in anoxic sediment slurries collected from the Keelung River was investigated in this study. o,p'- and p,p'-DDT were dechlorinated to o,p'- and p,p'-DDD, respectively, and then transformed to other compound(s). 1-Chloro-2,2-bis (p-chlorophenyl) ethylene (DDMU) and trace amount of dichlorobenzophenone (DBP) were detected in sediment slurries amended with p,p'-DDT or p,p'-DDD. DDMU was also detected in sediment slurries amended with p,p'-DDE. The relative transformation rates for both o,p'- and p,p'-isomers of DDT, DDD, and DDE were DDT>DDD>DDE. Re-addition of DDT, DDD, or DDE to the sediment slurries after initial removal enhanced the respective dechlorination rates. The transformation rates of the p,p'-isomers of both DDT and DDD were faster than those of the respective o,p'-isomers. p,p'-DDT dechlorination in the p,p'-DDT-adapted sediment slurries were inhibited by the addition of molybdate, or molybdate plus sulfate, but not inhibited by the addition of sulfate. Addition of bromoethane-sulfonic acid (BESA) slightly inhibited p,p'-DDT dechlorination. Non-adapted sediment slurries lost the ability to dechlorinate pentachlorophenol during adaptation to p,p'-DDT. p,p'-DDD was the major transformation product of p, p'-DDT in 3,4,4',5-tetrachlorobiphenyl-adapted sediment slurries, which suggested that the microbial community in the 3,4,4',5-CB-adapted sediment was unable to remove chlorine from the aromatic rings of p,p'-DDT.
Assuntos
DDT/metabolismo , Diclorodifenil Dicloroetileno/análogos & derivados , Diclorodifenil Dicloroetileno/metabolismo , Diclorodifenildicloroetano/metabolismo , Inseticidas/metabolismo , Resíduos de Praguicidas/metabolismo , Ácidos Alcanossulfônicos/farmacologia , Anaerobiose , Biodegradação Ambiental , Carbono/análise , DDT/análogos & derivados , Diclorodifenildicloroetano/análogos & derivados , Água Doce/análise , Água Doce/microbiologia , Sedimentos Geológicos , Concentração de Íons de Hidrogênio , Molibdênio/farmacologia , Nitrogênio/análise , Pentaclorofenol/metabolismo , Poluentes do Solo/metabolismo , Sulfatos/farmacologia , Taiwan , Poluentes Químicos da Água/metabolismoRESUMO
In this study, we investigated the biodegradability of biphenyl and 5 congeners (one non-planar and four coplanar) of polychlorinated biphenyl (PCB). Biphenyl, the non-planar congener 2,3',4',5-tetrachlorobiphenyl (25-34 CB), and the four coplanar congeners 3,3',4,4'-tetrachlorobiphenyl (34-34 CB), 3,4,4',5-tetrachlorobiphenyl (345-4 CB), 3,3',4,4',5-pentachlorobiphenyl (345-34 CB), and 3,3',4,4',5,5'-hexachlorobiphenyl (345-345 CB) were amended at a concentration of 10 mg/L into anoxic sediment slurries collected from the estuaries of the Tansui River and the Erjen River. During 2 years' incubation under sulfidogenic conditions, biphenyl was persistent, while all other chlorinated congeners, except for 345-345 CB, were dechlorinated with or without a lag period in sediment slurries collected from both rivers. Dechlorination of coplanar and non-planar congeners began with para chlorine removal. All para chlorines from the mono-, di-, and trichlorobiphenyl groups could be removed by sediment slurries from both rivers. Microbial communities in sediment from the Erjen River additionally fostered meta-dechlorination activity, but only after removal of all the para chlorines. Addition of Tween 20 (0.05%, v/v) into sediment slurries from the Tansui River did not enhance dechlorination rates or extents, but the addition of toluene- or 3-chlorobenzoate-adapted sediments enhanced dechlorination of 34-34 CB and 345-4 CB.