RESUMO
Transmission electron microscopy has revealed the capacity of rotaviruses for adsorption on Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis cells and the absence of such capacity with respect to Enterococcus faecalis, Lactobacillus casei and Edwardsiella tarda. Different degrees and a varying character of the adsorption of rotaviruses by the representatives of the opportunistic group of bacteria have been established, which may aggravate the course of rotavirus diarrhea due to the aggregation of viral particles. For the first time the phenomenon of the selective destructive adsorption of rotaviruses by E. coli indigenous strains M-17 and 33/29, typical representatives of normal enteric microflora, is described. This observation should be regarded as one of important biological mechanisms responsible for the protection of the body from rotavirus infection.
Assuntos
Enterobacteriaceae/ultraestrutura , Intestinos/microbiologia , Rotavirus/ultraestrutura , Vírion/ultraestrutura , Adsorção , Criança , Diarreia/microbiologia , Enterobacteriaceae/isolamento & purificação , Fezes/microbiologia , Humanos , Microscopia Eletrônica , Rotavirus/isolamento & purificação , Infecções por Rotavirus/microbiologia , Vírion/isolamento & purificaçãoRESUMO
The adsorbing activity of granulated carbonic sorbents SKN and KAU, as well as their oxidated forms, containing protogenic carboxylic and phenolic groups with respect to Shigella flexneri, Salmonella typhimurium, Escherichia coli, Streptococcus aureus and Pseudomonas aeruginosa pathogenic strains has been studied. As shown in this study, the process of interaction between microorganisms and carbonic sorbents has two stages. At the first stage the main role is played by long-distance electrostatic forces and at the second stage, by Van der Waals short-distance forces, as well as bonds formed between cell structures and surface groupings of carbonaceous materials. In the mechanism of interaction between microbial cells and carbons the geometry of carbon surface plays an important role. KAU(0)-1 exhibits the highest degree of adhesion with respect to pathogenic bacteria.
Assuntos
Aderência Bacteriana , Desintoxicação por Sorção , Adsorção , Carvão Vegetal , Escherichia coli/patogenicidade , Porosidade , Pseudomonas aeruginosa/patogenicidade , Salmonella typhimurium/patogenicidade , Shigella flexneri/patogenicidade , Staphylococcus aureus/patogenicidade , Propriedades de SuperfícieAssuntos
Intestino Delgado/microbiologia , Orthomyxoviridae/isolamento & purificação , Doenças dos Suínos/microbiologia , Vírion/isolamento & purificação , Aborto Animal/microbiologia , Animais , Animais Recém-Nascidos , Anticorpos Antivirais/análise , Feminino , Morte Fetal/microbiologia , Morte Fetal/veterinária , Microscopia Eletrônica , Orthomyxoviridae/imunologia , Orthomyxoviridae/ultraestrutura , Gravidez , Suínos , Vírion/imunologia , Vírion/ultraestruturaRESUMO
In experiments on human fetal intestinal explants infected with shigellae the specific multiplication rate of these infective agents, found to be 0.026, and the maximum level of their accumulation in erythrocytes, reaching 22-36 microbes per cell, have been determined. These phenomena can be observed after at least 3-hour incubation and end in the release of the infective agents from the affected area with shedding epithelial elements (villi). Shigellae, aggregated in the shed villi easily adhering to the unaffected mucosa, ensure the intensive invasion of the epithelium, which leads to the continuation of the process. The regularity thus revealed indicates that the population cycle of the development of shigellae is limited by short intervals of 3-4 hours. During these intervals the repeated invasion and the release of shigellae, together with the shed epithelium, into the chyme-containing intestinal cavity occur. The conditions for the multiplication of shigellae and their specific multiplication rate in chyme are minimal (0.016).
Assuntos
Shigella flexneri/crescimento & desenvolvimento , Shigella sonnei/crescimento & desenvolvimento , Adesividade , Divisão Celular , Epitélio/microbiologia , Feto , Humanos , Técnicas In Vitro , Mucosa Intestinal/microbiologia , Microscopia Eletrônica , Shigella flexneri/patogenicidade , Shigella sonnei/patogenicidade , Fatores de TempoRESUMO
The study of the adhesion of Shigella flexneri to intestinal mucosal explants from human postabortion fetuses, used as an experimental model, has revealed that the process of interaction between the infective agent and the epithelium develops in accordance with Langmuir's equation of the adsorption isotherm. The specific biological feature of the adhesive interaction between bacteria and the mucous membrane is the fact that the effective adhesion of microbes is possible only in case of their high concentration on the surface of the mucous membrane. In case of their low concentration in the parietal layer no microbial adhesion is observed, whereas epithelial villi infected with shigellae and fixed to the mucous membrane of the explant produce a high parietal concentration of the infective agents, which leads to the increase of adhesion by more than two orders.
Assuntos
Disenteria Bacilar/etiologia , Shigella flexneri/patogenicidade , Adesividade , Adulto , Animais , Disenteria Bacilar/microbiologia , Epitélio/microbiologia , Feto , Humanos , Mucosa Intestinal/microbiologia , Intestinos/microbiologia , Camundongos , Técnicas de Cultura de Órgãos , Traqueia/microbiologiaAssuntos
Gastroenterite/veterinária , Infecções por Reoviridae/veterinária , Reoviridae/ultraestrutura , Rotavirus/ultraestrutura , Doenças dos Suínos/microbiologia , Animais , Animais Lactentes , Gastroenterite/microbiologia , Microscopia Eletrônica , Infecções por Reoviridae/microbiologia , SuínosAssuntos
Coronaviridae/isolamento & purificação , Gastroenterite Suína Transmissível/microbiologia , Vírus da Gastroenterite Transmissível/isolamento & purificação , Animais , Microscopia Eletrônica , Testes de Neutralização , Suínos , Vírus da Gastroenterite Transmissível/imunologia , Vírus da Gastroenterite Transmissível/ultraestruturaAssuntos
Coronaviridae/isolamento & purificação , Suínos/microbiologia , Animais , Anticorpos Antivirais/análise , Coronaviridae/imunologia , Feminino , Gastroenterite Suína Transmissível/microbiologia , Gastroenterite Suína Transmissível/patologia , Microscopia Eletrônica , Cultura de Vírus/métodosAssuntos
Toxinas Biológicas/toxicidade , Vibrio cholerae/patogenicidade , Animais , Anuros , Membrana Basal/ultraestrutura , Cólera/etiologia , Cólera/patologia , Citoplasma/ultraestrutura , Endotoxinas/toxicidade , Cobaias , Humanos , Intestinos/ultraestrutura , Camundongos , Microscopia Eletrônica , Coelhos , Fatores de Tempo , Vibrio cholerae/ultraestruturaAssuntos
Disenteria Bacilar/patologia , Infecções por Salmonella/patologia , Animais , Membrana Celular/ultraestrutura , Células Cultivadas , Disenteria Bacilar/microbiologia , Endotoxinas/intoxicação , Epitélio , Cobaias , Humanos , Mucosa Intestinal/microbiologia , Mucosa Intestinal/ultraestrutura , Lisossomos/ultraestrutura , Macrófagos/ultraestrutura , Camundongos , Microscopia Eletrônica , Mitocôndrias/ultraestrutura , Ratos , Infecções por Salmonella/microbiologia , Shigella flexneri , Febre Tifoide/patologiaAssuntos
Anestesia por Inalação , Éter/farmacologia , Etil-Éteres/farmacologia , Halotano/farmacologia , Fígado/ultraestrutura , Mitocôndrias Hepáticas/metabolismo , Animais , Feminino , Glicogênio/metabolismo , Lactatos/metabolismo , Masculino , Mitocôndrias Hepáticas/ultraestrutura , Piruvatos/metabolismo , RatosRESUMO
Experiments were conducted on 117 rabbits and cells of the macrophage cultures in vitro by the methods of clinico-laboratory, quantitative microbiological, immunological, electron microscopic and microcinematographic examination; a study was made of the interaction of the typhoid causative agent with the cells of the organism and the macrophage cultures and also of some aspects of the immune response during acute typhoid infection and carrier state. Infection was modelled by the enteral, subconjunctival and intrabonemarrow infection with 24-hour culture of the typhoid bacillus (strain Ty2 4446). Experiments demonstrated that structural reconstruction of both the causative agent and of the cells of the organism, of the culture macrophages and their organoids occurred in the course of the first hour after the infection. Homogenates of the lymphoid and myeloid tissues and also of the macrophages and polymorphonuclears possessed bactericidal activity against S. typhi. The degree of this activity largely depended on the pH of the medium. It was also shown that under conditions of the macrophage culture sodium aside inhibited the bactericidal activity of macrophages obtained from the intact and immune animals.