RESUMO
Non-equilibrium atmospheric pressure plasma (NEAPP) is a mixture of radicals, electrons, anions, cations and light at near body temperature. Plasma-activated medium (PAM) is realized using NEAPP provided by engineered devices and irradiated to a cell culture medium for a period of 600â¯s. Glioblastoma cells U251SP cultivated in PAM previously indicated that antitumor effects induced PAM-specific apoptotic cell-death. Metabolomic profiles of a hundred intracellular metabolites were analyzed using capillary electrophoresis mass spectrometry. The metabolomic profiles of the PAM-treated U251SP cells were changed significantly with inhibition of the glycolysis pathway and with enhancement of the pentose phosphate pathway.
Assuntos
Neoplasias Encefálicas/metabolismo , Glioblastoma/metabolismo , Metabolômica , Gases em Plasma , Neoplasias Encefálicas/patologia , Linhagem Celular Tumoral , Meios de Cultura , Glioblastoma/patologia , Glicólise , Humanos , Via de Pentose FosfatoRESUMO
The multiplex coherent anti-Stokes Raman scattering microscopy allowed label-free visualization of cytoplasmic lipid droplets (LDs). The LDs, which act to conserve energy storage, are usually accumulated during the normal apoptosis of HeLa cells with activation of caspase-3/7 leading to downregulation of the fatty acid catabolism pathways. During cultivating in nonthermal plasma-activated medium (PAM), while the activation of caspase-3/7 was induced, the authors found that a dynamic exhaustion of the intracellular LDs, underlying the metabolic mechanism of the PAM-induced apoptotic cell death of HeLa cells.
Assuntos
Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Caspase 7/metabolismo , Gotículas Lipídicas/metabolismo , Microscopia Óptica não Linear , Gases em Plasma/farmacologia , Ativação Enzimática/efeitos dos fármacos , Células HeLa , HumanosRESUMO
Interactions between non-equilibrium atmospheric-pressure plasma (NEAPP) and living cells were examined using multiplex coherent anti-Stokes Raman scattering (CARS) microscopy. Our multiplex CARS analyses revealed that NEAPP irradiation generates short-lived radicals that induce a decrease in the mitochondrial activity of budding yeast cells.
Assuntos
Gases em Plasma/efeitos adversos , Saccharomyces cerevisiae/efeitos da radiação , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/efeitos da radiação , Microscopia Óptica não Linear , Gases em Plasma/química , Espécies Reativas de Nitrogênio/efeitos adversos , Espécies Reativas de Oxigênio/efeitos adversos , Saccharomyces cerevisiae/química , Raios UltravioletaRESUMO
Non-equilibrium atmospheric pressure plasmas generate a high electron density (on the order of 10(16) electrons per cm(-3)) using Ar gas. Culture medium in air at room temperature was plasma-irradiated for several hundred seconds. Tens of micromolar hydrogen peroxide (H2O2) and millimolar levels of nitrous ion (NO2(-)) were detected in the plasma-irradiated culture medium (plasma activated medium; PAM) and selectively induced the apoptotic death of glioblastoma tumor cells, but did not kill normal mammary epithelial cells. A similar antitumor effect was induced by spiking the medium with comparable concentrations of H2O2 and NO2(-). The PAM remained still a somewhat difference that it should also be assessed for understanding other latent mechanisms.
