[Selective modification of T7 DNA at the region of early genes by early RNA carrying multiple alkylating groups]. / Napravlennaia modifikatsiia DNK bakteriofaga T7 v oblasti rannikh genov s pomoshch'iu komplementarnogo RNK-transkripta, nesushchego mnozhestvennye alkiliruiushchie gruppy.

A method of selective modification of certain regions of the genome which may become useful for inactivation of certain genes or for directed mutagenesis is proposed. For this purpose RNA products of certain genes carrying alkylating groupings randomly distributed along the polymer were used. The RNA modified to an extent of 4--5 alkylating residues per 100 nucleotides retains the ability to specific formation of DNA--RNA hybrid complexes. The alkylating molecule is N,N,N'-tri-(beta-chlorethyl), N'-(p-formylphenyl)propylene diamine-1,3. The aliphatic alkylating functions serve for attachment to RNA. The aromatic alkylating function inactivated by the formyl grouping at the para-position of the benzene ring is used for modification of DNA after hybrid formation by reduction of formyl grouping with sodium borohydride. The covalently binding of modified RNA is exhibited to occur in only the case of T7 DNA H-chain, the one complementary to the RNA derivative. L-chain does not hybridize, nor does it undergo alkylation by the RNA product thus indicating high selectivity of alkylation within the hybrid complex.

[Affinity alkylation of E. coli RNA-dependent DNA polymerase with TTP gamma-amidate derivatives]. / Affinnoe alkilirovanie RNK-zavisimoi DNK-polimerazy E. coli proizvodnym gamma-amida TTR.

TTP gamma-benzylamidates are shown to act as competitive inhibitors of poly(dT) synthesis catalyzed by E. coli RNA-dependent DNA polymerase. The KM value for TTP as well as KI values for the gamma-analogues have been determined. TTP gamma-4-(N-2-chloroethyl-N-methyl-amino)benzylamidate is shown to be an effective affinity reagent for this enzyme.