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[This corrects the article DOI: 10.1186/s13027-019-0262-5.].
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ABSTRACT: Earlier we suggested a new hypothesis of the possible evolutionary role of hereditary tumors (Kozlov, Evolution by tumor Neofunctionalization, 2014), and described a new class of genes - tumor specifically expressed, evolutionarily novel (TSEEN) genes - that are predicted by this hypothesis (Kozlov, Infect Agents Cancer 11:34, 2016). In this paper we studied evolutionarily novel genes expressed in fish tumors after regression, as a model of evolving organs. As evolutionarily novel genes may not yet have organismal functions, we studied the acquisition of new gene functions by comparing fish evolutionarily novel genes with their human orthologs. We found that many genes involved in development of progressive traits in humans (lung, mammary gland, placenta, ventricular septum, etc.) originated in fish and are expressed in fish tumors and tumors after regression. These findings support a possible evolutionary role of hereditary tumors, and in particular the hypothesis of evolution by tumor neofunctionalization. RESEARCH HIGHLIGHTS: Earlier we described a new class of genes that are tumor-specifically expressed and evolutionarily novel (TSEEN). As the functions of TSEEN genes are often uncertain, we decided to study TSEEN genes of fishes so that we could trace the appearance of their new functions in higher vertebrates. We found that many human genes which are involved in development of progressive traits (placenta development, mammary gland and lung development etc.,) originated in fishes and are expressed in fish tumors.
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In this paper we have showed that evolutionary new genes DCD1(Dermicidin), LINC00309 (Long intergenic non-protein coding RNA 309) and CLLU1(Chronic lymphocytic leukemia up-regulated 1) have tumor-specific expression profile. Along with our previously published results this confirms the existence of the phenomenon of TSEEN (Tumor-Specifically Expressed, Evolutionarily Novel).
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Proteínas de Neoplasias/genética , Neoplasias/genética , Peptídeos/genética , RNA Longo não Codificante/genética , Biomarcadores Tumorais/genética , Evolução Molecular , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Neoplasias/classificação , Neoplasias/patologiaRESUMO
Cells from anti-HIV-positive persons were used in experiments for virus isolation. The RT-activity, viral antigen, nucleic acids, electron microscopic morphology, and infectivity were studied. The data presented allow a conclusion that the virus was isolated. These data confirmed the previous diagnosis of HIV infection.
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Antígenos HIV/sangue , Soropositividade para HIV/imunologia , HIV-1/imunologia , Linfócitos/imunologia , Northern Blotting , Southern Blotting , Linhagem Celular , Células Cultivadas/imunologia , Células Cultivadas/microbiologia , DNA Viral/genética , Soropositividade para HIV/microbiologia , HIV-1/genética , HIV-1/ultraestrutura , Humanos , Linfócitos/microbiologia , Microscopia Eletrônica , Hibridização de Ácido Nucleico , RNA Viral/genética , DNA Polimerase Dirigida por RNA/análise , Vírion/ultraestruturaRESUMO
The data are presented on the cloning and structural analysis of the cDNA coding for human prointerleukin-1 alpha and prointerleukin-1 beta (proIL-1 alpha and proIL-1 beta). The nucleotide sequences of proIL-1 alpha and proIL-1 beta cDNAs have been compared with the sequences published earlier. The nucleotide changes resulting in the aminoacid changes of the protein were not found. Some nucleotide changes were identified within the 3'-nontranslated region of the proIL-1 beta cDNA. The existence of the allelic variants for interleukin genes registered only on the gene level has been supposed.
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Clonagem Molecular , DNA/genética , Interleucina-1/genética , Precursores de Proteínas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Humanos , Interleucina-1/farmacologia , Ativação Linfocitária/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos CBA , Dados de Sequência Molecular , Precursores de Proteínas/farmacologiaRESUMO
Blot hybridization of 125I or 32P labeled 4.5S, U1 or U2 RNAs with EcoRI, HindIII, BamHI or SalG1 restriction fragments of high molecular weight DNA was performed. All these RNAs hybridized with fragments of ribosomal DNA and with 5.55-kb BamHI lines of rat. U1 ind 4.5S RNAs hybridized also with 3.6-kb HindIII lines. 125I labeled U1 RNA was hybridized with nuclear RNA in formamide. The hybrid molecules were formed which migrate in polyacrylamide gel as a broad peak slower than 28S rRNA. Our data indicate that snRNAs may participate in processing and/or splicing of hnRNA and in some other still poorly understood processes.
