[Study of the structure-activity organization of the variola virus genome. II. Analysis of the nucleotide sequence of the HindIII region (C,E,R,Q,K and H)-DNA fragments of the India-1967 strain]. / Izuchenie strukturno-funktsional'noi organizatsii genoma virusa natural'noi ospy. II. Analiz posledovatel'nosti nukleotidov raiona HindIII (C,E,R,Q,K i H)-fragmentov DNK shtamma india-1967.

Sequencing of variola virus (VAR) genome region of 43069 bp was carried out. This area contains 42 potential genes. Computer analysis of proteins coding for these viral genes was done. We compared VAR proteins with the those of vaccinia virus. The region studied is conservative for orthopoxviruses.

[Cloning the gene for vaccinia virus strain L-IVP growth factor in Escherichia coli]. / Klonirovanie v kletkakh Escherichia coli gena faktora rosta virusa ospovaktsiny shtamma L-IVP.

The growth factor gene of the vaccinia virus LIVP strain has been primarily cloned in a 4.3 kbp long BamHI-EcoRI fragment and then subcloned in a 440 bp fragment. It was shown that clone 4 of the LIVP strain contains a single copy of this gene while the WR strain contains a repeat. The gene is located on a 4.3 kbp BamHI-EcoRI fragment but not on a 2.2 kbp fragment and has four nucleotide changes, three of which result in amino acid substitutions.