Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Arch Toxicol ; 86(12): 1841-50, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22872140

RESUMO

Sodium diethyldithiocarbamate (DETC) is the main metabolite of disulfiram. Recently, we reported that mechanism of disulfiram cytotoxicity in V79 cells might be partially connected with thiol redox-state imbalance. Here, we examined the effect of DETC on the level of intracellular glutathione (GSH), protein oxidation (measured as PC-protein carbonyl content), lipid peroxidation (measured as TBARS-thiobarbituric acid reactive substances), antioxidant enzymatic defense, as well as on apoptosis. We used V79 Chinese hamster fibroblasts cells with and without modulated glutathione (GSH) level by N-acetyl-L-cysteine (NAC). We showed that treatment with DETC at concentrations that cause a moderate increase in thiol-state imbalance but not cell death stimulates oxidative stress measured as increased level of PC and TBARS, adaptive response of GSH-related enzymes and apoptosis. Our results show that cellular effects of DETC are partially attributable to the initial redox cellular state, since the increase of GSH level by NAC pre-treatment prevented the observed changes.


Assuntos
Antioxidantes/metabolismo , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ditiocarb/toxicidade , Fibroblastos/efeitos dos fármacos , Glutationa/metabolismo , Animais , Anexina A5 , Catalase/metabolismo , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Colorimetria , Corantes , Cricetinae , Fragmentação do DNA/efeitos dos fármacos , Dissulfiram/toxicidade , Fibroblastos/enzimologia , Glutationa Peroxidase/metabolismo , Glutationa Redutase/metabolismo , Marcação In Situ das Extremidades Cortadas , Peroxidação de Lipídeos/efeitos dos fármacos , Carbonilação Proteica , Substâncias Reativas com Ácido Tiobarbitúrico , Azul Tripano
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...