RESUMO
We have isolated cDNA encoding a novel FGF (212 amino acids) from rat brain. Because this is the 20th documented member of the FGF family, we tentatively term it FGF-20. Among FGF family members, FGF-20 is most similar to FGF-9 and FGF-16 (70 and 62% amino acid identity, respectively). Human FGF-20 gene was found in the human genomic sequence mapped to the 8p21.3-p22 region. Human FGF-20 is highly identical to rat FGF-20 (95% amino acid identity). FGF-20 mRNA was preferentially expressed in rat brain among the adult major tissues examined. The localization of FGF-20 mRNA in rat brain was also examined by in situ hybridization. FGF-20 mRNA was preferentially expressed in the substantia nigra pars compacta. To examine the biological activity of FGF-20, recombinant rat FGF-20 was produced by insect cells infected with recombinant baculovirus containing rat FGF-20 cDNA. Recombinant rat FGF-20 enhanced the survival of midbrain dopaminergic neurons. The present results indicate that FGF-20 is a novel neurotrophic factor preferentially expressed in the substantia nigra pars compacta of rat brain.
Assuntos
Encéfalo/metabolismo , Fatores de Crescimento de Fibroblastos/biossíntese , Substância Negra/metabolismo , Sequência de Aminoácidos , Animais , Baculoviridae , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Mapeamento Cromossômico , Cromossomos Humanos Par 8 , DNA Complementar/metabolismo , Dopamina/metabolismo , Relação Dose-Resposta a Droga , Evolução Molecular , Fator 9 de Crescimento de Fibroblastos , Fatores de Crescimento de Fibroblastos/química , Ácido Glutâmico/toxicidade , Substâncias de Crescimento/química , Humanos , Hibridização In Situ , Insetos , Dados de Sequência Molecular , Filogenia , Ratos , Ratos Wistar , Proteínas Recombinantes/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos , Distribuição Tecidual , TransfecçãoRESUMO
Les auteurs rapportent 39 cas d'anevrysmes diagnostiques sur une base d'explorations invasives et non invasives. L'etiologie est retenue apres examens biologiques; histopathologiques et paracliniques. En dessous de 50 ans les femmes sont les plus atteintes (1H/2F) surtout par la panarterite specifique (Maladie de Takayasu). Au dela de cet age c'est l'atherosclerose qui est plutot masculine (2H/1F). L'hypertension arterielle et l'hyperccholesterolemie totale sont presentes chacune dans 8 cas (32 pour cent). 24 patients sont operes avec succes: 17 protheses en Dacon ou Teflon et 3 autogreffes veineuses
