1.
Bull Exp Biol Med
; 152(3): 344-7, 2012 Jan.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22803082
RESUMO
Expression of heparanase-1 in prostate tumors was evaluated by RT-PCR, immunoblotting, and immunohistochemistry. Malignant transformation was shown to be associated with considerable increase in the expression of heparanase-1 at both mRNA and protein levels, which correlated with the degree of metastasizing and can be used as the marker for diagnostics of the metastatic process.
Assuntos
Regulação Enzimológica da Expressão Gênica/fisiologia , Regulação Neoplásica da Expressão Gênica/fisiologia , Glucuronidase/metabolismo , Metástase Neoplásica/diagnóstico , Neoplasias da Próstata/metabolismo , Primers do DNA/genética , Humanos , Immunoblotting/métodos , Imuno-Histoquímica/métodos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
2.
Bull Exp Biol Med
; 151(2): 264-7, 2011 Jun.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22238765
RESUMO
We studied in vitro effect of epithermal neutrons in various doses on viability of glioblastoma U87 tumor cells. Increasing the dose from 1.9 to 4.1 Sv promoted cell death. Cytofluorimetric analysis revealed no activation of apoptosis in the irradiated cells, which attested to necrotic death of the tumor cells exposed to epithermal neutron radiation.
