[Analysis of peculiarities in nucleotides disposition in the origin of chromosome replication-oriC from Escherichia coli]. / Analiz osobennostei raspredeleniia nukleotidov na uchastke nachala replikatsii khromosomy-oriC iz Escherichia coli.

Along with symmetrical features (palindromes, direct and inverted repeats), periodicities in the disposition of nucleotides in the origin of chromosome replication oriC from E. coli were studied by means of Fourier analysis. Peaks corresponding to the periods T = 2, 17, 95-100 nucleotides are the highest in the Fourier spectrum of oriC. Peaks corresponding to the periods T = 3, 11, 19, 13, 24, 27, 28, 41, 79-81 nucleotides are also prominent, but not so high. Thus, the main periodicities of the oriC spectrum of are not multiple of the B-DNA sugar-phosphate backbone period, which destabilize DNA at oriC and contributes to the spontaneous unwinding of DNA. The differences between the Fourier spectrum of oriC and those of regions adjacent to oriC are demonstrated.

[Periodicity in contacts of RNA-polymerase with promotors]. / Periodichnosti v kontaktakh RNK-polimerazy s promotorami.

Periodicities in the position of E.coli RNA polymerase promoter contacts on several promoters (lacUV5, T7 A3, tetR, lambda cin, lambda c17, RNA1, and trp S.t.) were found by means of Fourier analysis. The comparison of the Fourier spectrum of core RNA polymerase contacts on the lacUV5 promoter and that of holoenzyme revealed a more prominent 7-periodicity in the Fourier spectrum of holoenzyme contacts. 6-, 7-, and 8-periodicities were found in the primary structure of the majority of E.coli promoters. It is shown that RNA polymerase recognizes specific periodic patterns in the promoter structure.

[Fourier analysis of nucleotide sequences. Periodicity in E. coli promoter sequences]. / Fur'e-analiz nukleotidnykh posledovatel'nostei. Periodichnosti v promoternykh posledovatel'nostiakh E. coli.

Fourier spectra of E. coli promoter DNA sequences have been obtained. The periodical structure of individual promoter sequences is characterized. E. coli promoter sequences are classified according to their Fourier spectra using three feature sets: 1--the number of peaks in Fourier spectra; 2--values of power spectra for promoter primary structures and their similarity with physical periodicities in the backbone of polynucleotide; 3--the presence of blocks made of equal nucleotides. The comparison of Fourier spectra of promoter sequences and corresponding genes is provided. The conclusion that different ways of stabilization of promoter secondary structure in the case of different primary structure periodicities is drawn. The intermittence of AT- and GC-blocks, and variety of Fourier spectra mean DNA hydrate shell in DNA promoters is non-contiguous and non-stable at junction points. Characteristic features of prokaryotic promoters Fourier spectra differ from human promoters.

[An algorithm for searching for specific segments in the primary structure of DNA]. / Algorithm poiska spetsificheskikh uchastkov v pervichnoi strukture DNK.

A simple method of revealing peculiarities in the nucleic acid primary structure. Efficiency comparison between the method suggested and that one based on the use of Kohonen neural network has also carried out.