RESUMO
Experiments on cultured human umbilical vein endotheliocytes showed that accumulation of photosensitive dye (aluminum phthalocyanin; PHOTOSENSE) in cells and laser exposure alone were inessential for the viability of endothelial cells. Contrary to this, exposure of the cells which have accumulated aluminum phthalocyanin (an average of 111.1 ng/mg protein) to low-intensity laser (λ=675 nm) led to a dose-dependent reduction of endotheliocyte viability. Hence, cultured endothelial cells can be used for screening of various photosensitizers and preliminary optimization of photodynamic therapy.
Assuntos
Células Endoteliais/efeitos dos fármacos , Indóis/toxicidade , Terapia a Laser/métodos , Compostos Organometálicos/toxicidade , Fotoquimioterapia/métodos , Sobrevivência Celular/efeitos dos fármacos , Células Endoteliais/metabolismo , Humanos , Técnicas In Vitro , Indóis/farmacocinética , Microscopia de Fluorescência , Compostos Organometálicos/farmacocinética , Sais de Tetrazólio , Tiazóis , Veias Umbilicais/citologiaRESUMO
The ATHENA and ATRAP groups at CERN recently reported the production of weakly bound antihydrogen atoms in a non-neutral positron-antiproton plasma. This Letter derives an equation of motion for weakly bound atoms in the electric and magnetic fields of the plasma and trap. The atoms are polarized by the electric field and can be trapped radially in the edge region of the plasma where the electric field is maximum.
RESUMO
In recent experiments, ultracold plasmas were produced by photoionizing small clouds of laser-cooled atoms. It has been suggested that the low initial temperature of these novel plasmas leads directly to strong correlation and order. In contrast, we argue that rapid intrinsic heating raises the electron temperature to the point where strong correlation cannot develop. The argument is corroborated by a molecular-dynamics simulation of the early-time plasma evolution.
