[Modification of a catalase by glutaraldehyde].

Polycondensation of a catalase (EC 1.11.1.6) with glutaraldehyde in order to stabilize the quaternary structure of an enzyme, maintain its activity, and protect it from thermal denaturation was studied. Synthesis showed a superequivalent utilization of the aldehyde groups relative to the catalase amine groups, as a result of the formation of glutaraldehyde oligomers linked to the enzyme.

[The effect of fluvastatin (Lescol) treatment on the clinical status and function of the liver in patients with ischemic heart disease]. / Vliianie lecheniia fliuvastatinom (Leskolom) na klinicheskii status i funktsiiu pecheni bol'nykh ishemicheskoi bolezn'iu serdtsa.

The study included 30 IHD patients with primary hypercholesterolemia (22 males and 8 females). 18 and 12 patients have received a single daily dose of fluvastatin 20 and 40 mg, respectively, in the evening for 12 weeks. The drug effect was assessed by changes in the clinical status, lipid spectrum, transport-metabolic and absorption-secretory functions of the liver. IHD patients with hypercholesterolemia were found to have dysfunction of the hepatobiliary system. Fluvastatin treatment reduced the level of total cholesterol (Ch), LDLP Ch, triglycerides. HDLP Ch levels remained unchanged. Atherogenic lipoproteins aggregation diminished. Positive changes occurred in hepatic metabolism: bilirubin concentrations lowered, serum albumin went up, absorption-secretory function of hepatocytes normalized, hepatic mono-oxidase system activated. Fluvastatin-related hepatic damage was not reported in the course of 12-month follow-up.

[Epidemiological effectiveness of an inactivated three-component flu vaccine with an increased concentration of hemagglutinin in the inoculation dose]. / Epidemiologicheskia éffektivnost' inaktivirovannoi trekhkomponentnoigrippoznoi vaktsiny s povyshennoi kontsentratsiei gemaggliutinina v privivochnoi doze.

The work presents the results of the evaluation of mass immunization of working adults with inactivated trivalent influenza vaccine under the conditions of an epidemic caused by influenza viruses A (H1N1), A (H3N2) and B. This immunization produced no effect on influenza morbidity in the groups of vaccinees in comparison with those of nonvaccinated persons. The index of effectiveness was 1.0 and less. The ineffectiveness of mass immunization was due to a high level of natural immunity to influenza and the extensive use of influenza vaccine in past years.

[The evaluation of the reactogenicity and immunological activity of an inactivated 3-component influenza vaccine with an elevated hemagglutinin concentration in the inoculation dosage]. / Otsenka reaktogennosti i immunologicheskoi aktivnosti inaktivirovannoi trekhkomponentnoi grippoznoi vaktsiny s povyshennoi kontsentratsiei gemaggliutinina v privivochnoi doze.

In this work the reactogenic properties and antigenic potency of inactivated trivalent influenza vaccine, obtained by elution and centrifugation and containing up to 9-11 micrograms of hemagglutinin for influenza viruses A(H1N1) and A(H3N2) and up to 14 micrograms for influenza virus B, were studied. The reactogenicity of the preparation was found to correspond to the regulations. The immunogenic potency characteristics of individual batches of this trivaccine were higher than the immunogenicity of divaccines, but did not meet the requirements of technical specifications.

[The role of hemoglobin affinity for oxygen in the efficiency of the respiratory function of the blood]. / O roli srodstva gemoglobina k kislorodu v éffektivnosti dykahtel'noi funktsii krovi.

In acute experiments the oxygen transport properties of two solutions of modified stroma-purified hemoglobin were investigated using anesthetized Wistar rats. The solutions were: solution 1 (Hb = 8.0 g per 100 g, P50 = 12.5 mm Hg) and solution 2 (Hb = 4.4 g per 100 g, P50 = 21.5 mm Hg). The solutions were used in stage-by-stage isovolumic substitution in rats of two groups. The modified hemoglobin solution with a lower hemoglobin oxygen affinity was found to be a more efficient blood substitute. In spite of its low oxygen capacity, it could sustain life activity at very low hematocrit values. When the oxygen capacity of blood is moderate or low, hemoglobin oxygen affinity plays a very important part in oxygen supply to different tissues, specifically to the heart. The latter determines the crucial compensatory physiological reaction to acute anemia, i. e. increase of cardiac output.

[Diastolic filling of the heart ventricle]. / Diastolicheskoe zapolnenie zheludochkov serdtsa.

It is demonstrated that negative, "subatmospheric" pressure developing in the heart ventricles during the rapid-filling phase is an evidence of active diastole and inflated capacity of heart chambers. Diastolic activity of the heart increases as a result of insufficient blood inflow to the ventricles coupled with inadequate venous return or narrowed atrioventricular orifice. It is assumed that the heart's diastolic activity is an adaptive-compensatory mechanism adequately stabilizing circulation under changing hemodynamic conditions.