RESUMO
Induction of the P21 protein is an integral part of cell growth arrest associated with the cellular response to senescence or damage. Changes in the P21 protein level are often observed in tumour cells and it is suggested, that they correlate with malignancy. We studied the P21 gene expression at the mRNA level in biopsy specimens from colitis ulcerosa, adenomas and adenocarcinomas using TaqMan assay. Our results showed, that quantity of the P21 transcript was evidently lower in the cases with adenocarcinomas than in the cases with colitis ulcerosa or adenomas.
Assuntos
Adenoma/genética , Colite Ulcerativa/genética , Neoplasias do Colo/genética , Ciclinas/genética , Regulação Neoplásica da Expressão Gênica , Adenocarcinoma/genética , Adenocarcinoma/patologia , Adenoma/patologia , Biópsia , Transformação Celular Neoplásica , Colite Ulcerativa/patologia , Neoplasias do Colo/patologia , Inibidor de Quinase Dependente de Ciclina p21 , Humanos , RNA Mensageiro/análise , Taq PolimeraseRESUMO
In presented study, differences among six intestinal Desulfovibrio desulfuricans strains were investigated regarding their ability of sulphasalazine (SAS) biotransformation. Bacterial strains were incubated (24 or 48 h) in liquid medium supplemented with SAS (12.2-150 microM). It has been demonstrated that investigated D. desulfuricans strains converted SAS with various rates, depending on the strain used and on time of drug contact with bacterial cells. Significantly different (50% coefficient of variability) were the maximum rates of SAS biotransformation. It was demonstrated that these rates were strongly dependent on the drug concentration. Increasing SAS concentrations up to about 120 microM caused increase in the drug transformation rate. At higher SAS concentrations this rate remained at the same level or was inconsiderable lower. The strains exposed to SAS for 24 hs transformed this drug with the rate about twice as compared with that observed after 48 h exposition.
