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Acta Virol ; 34(2): 202-5, 1990 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-1698016

RESUMO

Viral (v) RNA was isolated from purified foot-and-mouth disease virus (FMDV) by phenol-chloroform-isoamylalcohol treatment, digested by RNase T1 and separated by one-dimensional polyacrylamide gel electrophoresis (PAGE). The oligonucleotides were detected by silver staining. About 45 micrograms of vRNA corresponding to about 100 ml of infectious BHK-21 cell culture fluid yielded a pattern of nearly 20 bands sufficient to differentiate between the FMD viruses.


Assuntos
Aphthovirus/genética , Oligonucleotídeos/análise , RNA Viral/análise , Animais , Células Cultivadas , Cricetinae , Febre Aftosa/genética , Ribonuclease T1 , Prata , Coloração e Rotulagem
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