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1.
J Vet Sci ; 11(3): 177-83, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20706023

RESUMO

Distribution and characterization of interlukin-10 (IL-10)-secreting cells in lymphoid tissues of pigs naturally infected with porcine circovirus type 2 (PCV2) were evaluated in accordance with PCV2 antigen detection. After screening a total of 56 pigs showing the symptoms of postweaning multisystemic wasting syndrome (PMWS), 15 pigs were PCV2 positive and 5 pigs, which showed stronger positive signals over multiples tissues were further investigated. This study showed that in PCV2-infected lymphoid tissues, particularly mandibular lymph node, spleen and tonsil, IL-10 expression was mainly localized in T-cell rich areas but rarely in B cell rich areas. IL-10 was highly expressed in bystander cells but rarely in PCV2-infected cells. Elevated IL-10 expression was predominantly associated with T cells, but rarely with B cells or with macrophages. The results of this study provide evidence for the role of IL-10 in chronic PCV2 infection and its relation to PCV2 antigen in affected tissues. Constantly elevated levels of IL-10 lead to immunosuppression in persistent and chronic viral infections. The increased IL-10 expression observed in PCV2 infection in this study suggests that IL-10-mediated immunosuppression may play an important role in the pathogenesis and maintenance of naturally occurring PCV2 infection.


Assuntos
Infecções por Circoviridae/veterinária , Circovirus/imunologia , Regulação da Expressão Gênica/imunologia , Interleucina-10/metabolismo , Tecido Linfoide/patologia , Síndrome Definhante Multissistêmico de Suínos Desmamados/imunologia , Animais , Infecções por Circoviridae/imunologia , Infecções por Circoviridae/patologia , Imuno-Histoquímica/veterinária , Interleucina-10/imunologia , Tecido Linfoide/imunologia , Tecido Linfoide/metabolismo , Síndrome Definhante Multissistêmico de Suínos Desmamados/patologia , República da Coreia , Suínos , Linfócitos T/imunologia
2.
J Vet Sci ; 11(1): 21-5, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20195061

RESUMO

In August 2008, forty dogs out of 400 developed oral warts in a breeding farm in Korea. Canine oral papilloma infection is a common disease in dogs. However, there has been no report of an outbreak of canine oral papillomavirus (COPV) in a group of dogs or in dog breeding farms in Korea, and the genetic analysis of COPV in Korea has yet to be performed. This study diagnosed canine oral papilloma from the oral samples of these dogs based on histopathological examination and immunohistochemistry. Polymerase chain reaction was applied to amplify the corresponding products using preexisting primer sets for COPV and a universal human papillomavirus targeting L1 gene. Further genetic analysis of the major viral capsid gene L1 confirms the sequences of Korean COPV, which shows a close relationship to previously reported COPV. This study describes the histopathological and immunohistochemical characteristics of canine oral papilloma in a group of breeding dogs in Korea and discloses the complete L1 gene sequences of Korean COPV.


Assuntos
Surtos de Doenças/veterinária , Doenças do Cão/virologia , Lambdapapillomavirus/isolamento & purificação , Doenças da Boca/veterinária , Infecções por Papillomavirus/veterinária , Animais , Sequência de Bases , Proteínas do Capsídeo/química , Proteínas do Capsídeo/genética , DNA Viral/química , DNA Viral/genética , Doenças do Cão/epidemiologia , Cães , Imuno-Histoquímica/veterinária , Coreia (Geográfico)/epidemiologia , Lambdapapillomavirus/genética , Dados de Sequência Molecular , Doenças da Boca/epidemiologia , Doenças da Boca/virologia , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/virologia , Reação em Cadeia da Polimerase/veterinária , Análise de Sequência de DNA
3.
J Virol Methods ; 130(1-2): 7-14, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16076499

RESUMO

The gene encoding the envelope glycoprotein (GP) of vesicular stomatitis virus serotype, Indiana (VSV-IN), was expressed under the polyhedron promoter of baculovirus. The recombinant GP was applied as a diagnostic antigen for the detection of cattle and horse antibodies to VSV. In addition, the neutralizing monoclonal antibody (Mab) to GP of VSV-IN was used as trapping antibody in a Mab-linked indirect ELISA (MLI-ELISA) or detecting antibody in a Mab-linked competitive ELISA (MLC-ELISA). The diagnostic efficiencies of MLI-ELISA and MLC-ELISA were evaluated with currently available C-ELISA from OIE reference laboratory for vesicular stomatitis as a gold standard by using VSV-positive equine sera and negative bovine sera vaccinated against foot-and-mouth disease (FMD) in the field. When naturally infected equine sera and FMDV vaccinated bovine sera were tested, MLI-ELISA and MLC-ELISA showed relative sensitivities of 80% and 95% with relative specificity of 97% and 99%, respectively. However, both ELISAs cross-reacted with equine sera against New Jersey (VSV-NJ) serotype. The comparison of the two ELISAs revealed that MLC-ELISA was relatively more sensitive and specific than MLI-ELISA, indicating that MLC-ELISA can be applied to sero-diagnosis for VSV-IN infection.


Assuntos
Ensaio de Imunoadsorção Enzimática/veterinária , Infecções por Rhabdoviridae/diagnóstico , Testes Sorológicos/veterinária , Vírus da Estomatite Vesicular Indiana/imunologia , Vesiculovirus , Animais , Anticorpos Monoclonais , Baculoviridae/metabolismo , Bovinos , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática/métodos , Glicoproteínas/biossíntese , Glicoproteínas/genética , Cavalos , Proteínas Recombinantes/biossíntese , Sensibilidade e Especificidade , Testes Sorológicos/métodos , Proteínas do Envelope Viral/biossíntese , Proteínas do Envelope Viral/genética
4.
J Virol Methods ; 113(1): 13-8, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14500122

RESUMO

VP7, the sero-group common antigen, of African horsesickness virus (AHSV-4) was expressed in insect cells by recombinant baculovirus. To develop a specific diagnostic method, monoclonal antibody (Mab) against VP7 was prepared and investigated as diagnostic reagent with the baculovirus expressed VP7. However, the Mab against VP7 of AHSV cross-reacted with Chuzan virus by the indirect immunofluorescence assay (IFA), confirming the presence of conserved domain of VP7 among Orbiviruses. This study describes two types of ELISA; Mab linked indirect (I-ELISA) and competitive-ELISA (C-ELISA) using baculovirus expressed VP7 as an antigen. These ELISAs were compared for serodiagnosis of AHSV showing that C-ELISA was more specific than I-ELISA. The results indicated that C-ELISA is applicable to serodiagnosis of AHSV regardless of serotypes.


Assuntos
Vírus da Doença Equina Africana/imunologia , Doença Equina Africana/diagnóstico , Anticorpos Antivirais/sangue , Antígenos Virais/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Proteínas do Core Viral/imunologia , Doença Equina Africana/imunologia , Doença Equina Africana/virologia , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/isolamento & purificação , Antígenos Virais/biossíntese , Antígenos Virais/genética , Antígenos Virais/isolamento & purificação , Clonagem Molecular , Genes Virais , Cavalos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Sensibilidade e Especificidade , Proteínas do Core Viral/biossíntese , Proteínas do Core Viral/genética , Proteínas do Core Viral/isolamento & purificação
5.
Virus Res ; 90(1-2): 15-22, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12457959

RESUMO

Molecular cloning and sequencing of the genome of foot-and-mouth disease virus (FMDV) O/SKR/2000, one of PanAsia strain, were performed from FMDV infected cattle. From the poly (C) tract of the 5' nontranslated region (NTR) to the 3' NTR including 14 base pairs (bp) of poly (A) tail, 7813 bp sequences comprising approximately 95% of the whole genome were obtained by reverse transcription polymerase reaction (RT-PCR). The deduced amino acid sequences of the structural and nonstructural proteins (NSP) of the O/SKR/2000 virus were analyzed for the sequence similarity among type O strains. Comparison between FMDV O/SKR/2000 and other strains indicates that overall the number of amino acids appears to be conserved without any deletion in either NSP or capsid proteins, thus, suggesting that O/SKR/2000 evolved with minor difference from preexisting strains.


Assuntos
Doenças dos Bovinos/virologia , Vírus da Febre Aftosa/genética , Febre Aftosa/virologia , Genoma Viral , Análise de Sequência de DNA , Sequência de Aminoácidos , Animais , Sequência de Bases , Bovinos , Clonagem Molecular , Vírus da Febre Aftosa/classificação , Dados de Sequência Molecular , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Proteínas Virais/química , Proteínas Virais/genética
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