Assuntos
Doenças do Gato/diagnóstico , Doenças da Gengiva/veterinária , Cistos Odontogênicos/veterinária , Animais , Biópsia por Agulha Fina/veterinária , Doenças do Gato/patologia , Gatos , Doenças da Gengiva/diagnóstico , Doenças da Gengiva/patologia , Masculino , Cistos Odontogênicos/diagnóstico , Cistos Odontogênicos/patologiaRESUMO
This report describes a dog infected with Hepatozoon canis, the first canine infection in the Republic of Korea. A 2-year-old intact male Maltese dog presented with anorexia and depression. Physical examinations revealed mild dehydration and hyperthermia (39.8°C), and blood analysis showed pancytopenia. Diff-Quik staining of blood smear specimens showed the presence of ellipsoidal shaped structures (gamonts of H. canis) within a small number of neutrophils. Real-time PCR analysis using whole blood confirmed infection by H. canis. The clinical condition of the dog improved after symptomatic treatment and administration of doxycycline. Although a molecular epidemiologic survey in Korea showed H. canis infection of dogs, to our knowledge this is the first report of a dog infection in Korea molecularly shown to be H. canis.
Assuntos
Apicomplexa , Doenças do Cão/diagnóstico , Doenças do Cão/parasitologia , Infecções Protozoárias em Animais/diagnóstico , Infecções Protozoárias em Animais/parasitologia , Animais , Doenças do Cão/tratamento farmacológico , Cães , Doxiciclina/administração & dosagem , Masculino , Infecções Protozoárias em Animais/tratamento farmacológico , Reação em Cadeia da Polimerase em Tempo Real , República da Coreia , Resultado do TratamentoRESUMO
Nestin is a protein of embryonic intermediate filaments expressed by multipotent neural stem cells. In the present study, the nestin expression pattern in the mouse hippocampus 1, 2, 3, 4, and 8 days after treatment with trimethyltin (TMT) was examined to explore the possible role played by nestin in chemically induced hippocampal injury. TMT treatment (2.5mg/kg, intraperitoneally) selectively injured the dentate gyrus (DG) of the mouse hippocampus. The level of hippocampal mRNA encoding nestin increased significantly 2 and 3 days post-treatment and thereafter decreased (at 4 and 8 days post-treatment). The level of nestin protein significantly increased 2 - 4 days post-treatment, particularly in the injured region of the DG, and predominantly in glial fibrillary acidic protein-positive astrocytes in the hippocampal DG. Ki67-positive proliferating cells were increased following TMT treatment and co-localized with nestin-positive reactive astrocytes. Thus, we suggest that nestin contributes to remodeling of the chemically injured DG via glial scar formation and the alteration of neurogenesis.
