RESUMO
Three antineoplastic agents, bleomycin, cytosine arabinoside and 5-fluorouracil, were studied individually and in combination to determine their effectiveness in controlling cell growth in the presence of fetal bovine serum. All three drugs were effective individually in specifically controlling proliferation (as measured by 3H-thymidine uptake) of human Tenon's capsule fibroblasts while bleomycin is effective at the lowest concentration. However, bleomycin alone rather than in combination with cytosine arabinoside or 5-fluorouracil was most effective in preventing division by these fibroblasts.
Assuntos
Antineoplásicos/farmacologia , Divisão Celular/efeitos dos fármacos , Animais , Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Bleomicina/farmacologia , Células Cultivadas , Citarabina/farmacologia , Avaliação Pré-Clínica de Medicamentos , Olho/citologia , Fibroblastos , Fluoruracila/farmacologia , Humanos , Coelhos , Pele/citologiaRESUMO
An assay is described to test the ability of antineoplastic agents to slow fibroblast growth. The effect of bleomycin sulfate, cytosine arabinoside (cytarabine), and 5-fluorouracil on suppression of fibroblast proliferation in 20% rabbit aqueous humor was studied in vitro. All of the drugs exhibited some suppression of fibroblast proliferation at differing doses. The choice of target cell, however, was an important factor in the dose responses obtained.
Assuntos
Antineoplásicos/farmacologia , Humor Aquoso/fisiologia , Inibidores do Crescimento/farmacologia , Animais , Bleomicina/farmacologia , Túnica Conjuntiva/citologia , Citarabina/farmacologia , Fibroblastos/efeitos dos fármacos , Fluoruracila/farmacologia , Humanos , Coelhos , Pele/citologiaRESUMO
The isoenzymes of aspartate transaminase differ in their kinetic properties in that the cytoplasmic isoenzyme is more readily inhibited by adipate and by 2-oxoglutarate (substrate) at low pH. A differential kinetic assay based on this phenomenon has been optimised for use in assays of serum samples. The new method agrees well with an immune absorption procedure. Methods based on chromatographic separation of the isoenzymes fail in the presence of serum.
