RESUMO
RATIONALE: Viremia has been implicated in many viral infections; however, viremia due to rhinovirus (RV; rhinoviremia) has been considered not to occur in normal individuals. OBJECTIVE: To evaluate whether RV enters the bloodstream and identify the possible risk factors. METHODS: Nasopharyngeal washes (NPWs) of 221 children with respiratory infections were examined for the presence of RV by reverse transcription-polymerase chain reaction. Blood from 88 children, whose NPW was RV-positive, and 31 of RV-negative control subjects was subsequently examined for the presence of RV in the blood by semi-nested reverse transcription-polymerase chain reaction. Rhinoviremia was then correlated with clinical characteristics of the disease. RESULTS: RV was detected in the blood of 10 out of 88 NPW RV-positive cases (11.4%): 7 of 28 children with asthma exacerbations (25.0%), 2 of 26 with common cold (7.7%), 1 of 25 with bronchiolitis (4.0%), and 0 of 9 with pneumonia (0%). All NPW RV-negative cases were negative in the blood. The proportion of rhinoviremia in children with asthma exacerbation was significantly higher compared with children suffering from the other diseases (25 vs. 5%, p = 0.01). Significant risk factors were: sampling Assuntos
Asma/virologia
, Bronquiolite/virologia
, Resfriado Comum/virologia
, Pneumonia Viral/virologia
, Rhinovirus/genética
, Viremia/virologia
, Doença Aguda
, Adolescente
, Asma/epidemiologia
, Bronquiolite/epidemiologia
, Estudos de Casos e Controles
, Criança
, Pré-Escolar
, Resfriado Comum/sangue
, Resfriado Comum/complicações
, Resfriado Comum/diagnóstico
, DNA Viral/análise
, DNA Viral/genética
, Eletroforese em Gel de Ágar
, Feminino
, Grécia/epidemiologia
, Hospitais Pediátricos
, Humanos
, Lactente
, Modelos Logísticos
, Masculino
, Análise Multivariada
, Líquido da Lavagem Nasal
, Pneumonia Viral/epidemiologia
, Reação em Cadeia da Polimerase Via Transcriptase Reversa
, Fatores de Risco
, Índice de Gravidade de Doença
, Viremia/sangue
, Viremia/complicações
, Viremia/diagnóstico