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1.
Mikrobiol Z ; 78(4): 90-101, 2016 Jul.
Artigo em Russo, Ucraniano | MEDLINE | ID: mdl-30653883

RESUMO

The features of the soybean symbiotic systems formation and carry out the complex es- timate of the rhizobium nodulation ability at the seed inoculation of the microbial composi- tions on the bases of nodule bacteria, azotobacter and phytolectins (soybean seeds lectin, wheat germ agglutinin) were studied in the green-house experiments with a soil cultures. It was shown, that complex inoculants accelerate the process of becoming infected of plants by rhizobia in the early stages of soybean development; contribute to the expansion of the spectrum of genetically determined ability of nodule bacteria in the formation of a certain number of nodules on the host plant during the growing season as well as the formation of more root nodules with more of their weight during the second half of the growing season of soybean and significant increase mass of the one nodule and also slow the root nodules aging process at the end of the growing season compared with a rhizobial monoinoculant. It was proposed to use a complex of criteria in the estimating of the rhizobia nodulation ability in the microbial compositions: "nodulation activity", "nodulation range", "the num- ber of nodules on the plant", "mass of nodules per plant", "mass of one nodule", which are indicative for different stages of the symbiosis formation.


Assuntos
Azotobacter/crescimento & desenvolvimento , Bradyrhizobium/crescimento & desenvolvimento , Glycine max/microbiologia , Nodulação/fisiologia , Sementes/microbiologia , Simbiose/fisiologia , Azotobacter/efeitos dos fármacos , Azotobacter/metabolismo , Bradyrhizobium/efeitos dos fármacos , Bradyrhizobium/metabolismo , Meios de Cultura/química , Meios de Cultura/farmacologia , Lectinas de Plantas/farmacologia , Nodulação/efeitos dos fármacos , Rizosfera , Sementes/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Proteínas de Soja/farmacologia , Glycine max/efeitos dos fármacos , Glycine max/crescimento & desenvolvimento , Simbiose/efeitos dos fármacos , Aglutininas do Germe de Trigo/farmacologia
2.
Mikrobiol Z ; 77(4): 2-7, 2015.
Artigo em Ucraniano | MEDLINE | ID: mdl-26422918

RESUMO

The isolates of microorganisms from the rhizosphere of spring barley plants and soil at the use of analytical selection method was isolated. Its isolates on the ability of "nitrogen-fixing activity" was tested. It was shown that isolates of microorganisms had different of the colonies formed and cultural growth on the Eshbi's selective medium as well as the ability to fixing of molecular nitrogen. The different levels of intensity and dynamics of isolates nitrogenase activity in vitro were identified. New isolates of the soil microorganisms complement of the gene pool diazotrophic bacteria. Its isolates are perspectivity for the study as the basis or components of the bacterial fertilizers for the crops.


Assuntos
Bactérias/isolamento & purificação , Proteínas de Bactérias/metabolismo , Hordeum/microbiologia , Nitrogenase/metabolismo , Microbiologia do Solo , Bactérias/enzimologia , Bactérias/genética , Proteínas de Bactérias/genética , Produtos Agrícolas/crescimento & desenvolvimento , Produtos Agrícolas/microbiologia , Fertilizantes/provisão & distribuição , Expressão Gênica , Pool Gênico , Hordeum/crescimento & desenvolvimento , Consórcios Microbianos/genética , Nitrogênio/metabolismo , Fixação de Nitrogênio/fisiologia , Nitrogenase/genética , Rizosfera , Simbiose/fisiologia
3.
Ukr Biokhim Zh (1999) ; 80(1): 107-13, 2008.
Artigo em Ucraniano | MEDLINE | ID: mdl-18710035

RESUMO

The response of spring wheat and rhizospheric nitrogen-fixing micro-organisms to the presowing treatment of seeds by wheat germ agglutinin was investigated in conditions of green house experiments. It was shown, that exogenous lectin induced the metabolic changes in plants and caused an increase in chlorophyll content and activity of endogenous lectins in the leaves, as well as enhanced accumulation of plants biomass and nitrogen-fixing capacity of the rhizospheric micro-organisms. These results evidence for the considerable role of exogenous lectin as a regulator of growth and development of plants and activity of the nitrogen-fixing microorganisms.


Assuntos
Clorofila/metabolismo , Nitrogênio/metabolismo , Triticum , Aglutininas do Germe de Trigo/farmacologia , Biomassa , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Lectinas de Plantas/metabolismo , Rizoma/efeitos dos fármacos , Rizoma/crescimento & desenvolvimento , Rizoma/microbiologia , Sementes/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Triticum/efeitos dos fármacos , Triticum/crescimento & desenvolvimento , Triticum/metabolismo , Triticum/microbiologia
4.
Mikrobiol Z ; 70(1): 17-24, 2008.
Artigo em Ucraniano | MEDLINE | ID: mdl-18416150

RESUMO

The influence of inoculation compositions on the basis of nodule bacteria Bradyrhizobium japonicum 634b and phytohormonal plant preparation Reglalg on formation, functioning and productivity of soybean-rhisobium symbiosis was studied in the vegetation and field experiments. It was shown, that the phytohormonal component of the composition increases the nodulation and nitrogenase activity of the Bradyrhizobium japonicum 634b and effectiveness of the symbiotic system.


Assuntos
Bradyrhizobium/crescimento & desenvolvimento , Glycine max/crescimento & desenvolvimento , Reguladores de Crescimento de Plantas/farmacologia , Nódulos Radiculares de Plantas/crescimento & desenvolvimento , Simbiose/efeitos dos fármacos , Agricultura , Biomassa , Bradyrhizobium/efeitos dos fármacos , Fixação de Nitrogênio/efeitos dos fármacos , Nódulos Radiculares de Plantas/efeitos dos fármacos , Nódulos Radiculares de Plantas/microbiologia , Glycine max/efeitos dos fármacos , Glycine max/microbiologia
5.
Ukr Biokhim Zh (1999) ; 78(2): 106-12, 2006.
Artigo em Ucraniano | MEDLINE | ID: mdl-17100292

RESUMO

The presawing treatment of wheat seeds by lectin specific to the plant and its compositions with gapten and nitrogen fixation bacteria on the change in enzyme activity of antioxidant system of plant protection (peroxydase and katalase), endogenous lectin activity and flavonoid content in wheat leaves was studied in vegetation experiments. It is shown, that different biochemical links of protection plant system are activated, i.e. lectin activity in leaves increases 1.3 to 1.5 times, peroxydase activity and katalase activity rises 1.5 times and 5.0 to 8.0 times respectively and flavonoide content in leaves of vegetating plants increases under normal conditions of plant growth at the influence of factors of lectin nature.


Assuntos
Antioxidantes/metabolismo , Catalase/metabolismo , Flavonoides/metabolismo , Peroxidase/metabolismo , Triticum , Aglutininas do Germe de Trigo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/enzimologia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Sementes/efeitos dos fármacos , Triticum/efeitos dos fármacos , Triticum/enzimologia , Triticum/crescimento & desenvolvimento , Triticum/metabolismo , Aglutininas do Germe de Trigo/metabolismo , Aglutininas do Germe de Trigo/farmacologia
6.
Ukr Biokhim Zh (1999) ; 78(6): 105-12, 2006.
Artigo em Ucraniano | MEDLINE | ID: mdl-17494326

RESUMO

The wheat lectin hemagglutination activity and degree of its interaction with the bacterium Azotobacter chroococcum T79 and aminosaccharide N-acetyl-D-glucosamin hapten of wheat lectin was studied in laboratory experiments with the purpose of creation of biologic activity compositions of lectin nature for plant growing. It was shown that plant-bacterial compositions encloses the "bacteria+lectin" complex, free lectin and bacterial cells. The addition of aminosaccharide N-acetyl-D-glucosamin to wheat lectin, to the bacterial culture and plant-bacterial composition decreases its hemagglutination activity. The possibility of creation of new complexes in this compositions effected by hapten "lectin+hapten", "lectin+hapten+bacteria", "bacteria+hapten" is under discussion.


Assuntos
Acetilglucosamina/química , Azotobacter/química , Haptenos/química , Triticum/metabolismo , Aglutininas do Germe de Trigo/química , Agregação Eritrocítica/efeitos dos fármacos , Hemaglutinação , Humanos , Técnicas In Vitro , Triticum/crescimento & desenvolvimento , Aglutininas do Germe de Trigo/metabolismo , Aglutininas do Germe de Trigo/farmacologia
7.
Ukr Biokhim Zh (1999) ; 77(4): 133-7, 2005.
Artigo em Ucraniano | MEDLINE | ID: mdl-16568616

RESUMO

The effect of presowing treatment of wheat seeds by exogenous wheat lectin on endogenous activity of lectin as well as on RNA content in vegetative organs of wheat was investigated. The variability of lectin activity in the seedling and leaves of wheat plants was obtained. Both endogenous activity of lectin and RNA amount increase under the effect of exogenous lectin. This effect was partially inhibited by the hapten of the wheat lectin. The possibility of induction of modifications in both endogenous lectin pool and functional activity of plant genome by exogenous lectin is under discussion.


Assuntos
Folhas de Planta/efeitos dos fármacos , RNA de Plantas/metabolismo , Plântula/efeitos dos fármacos , Triticum/efeitos dos fármacos , Aglutininas do Germe de Trigo/farmacologia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Triticum/crescimento & desenvolvimento , Triticum/metabolismo , Aglutininas do Germe de Trigo/metabolismo
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