RESUMO
Yellow band disease (YBD) is a common and wide-spread Caribbean syndrome that affects the genus Orbicella, a group of species that constitute the framework of Caribbean coral reefs. Previous studies have shown that the structure and function of bacterial assemblages vary between healthy tissues and YBD lesions; however, how the molecular composition of tissues varies as tissues transition from healthy to YBD has not been determined before. The present study provides the first survey of macromolecules found from healthy (H), apparently healthy (AH), transition (TR) and YBD tissues of Orbicella faveolata. For this, we used Fourier-transformed mid-infrared spectroscopy (FTIR) to compare absorption profiles as a proxy for the gross molecular composition of decalcified H, AH and YBD tissues. We found a significantly higher level of infrared absorption for bands assigned to lipids in H tissues compared to YBD tissues, suggesting that lipid compounds are more abundant in compromised tissues in relation to other macromolecules. We also found a lower level of intensity of bands assigned to carbohydrates and proteins in YBD tissues, compared to H and AH tissues. A similar pattern was observed for phospholipidic compounds in relation to fatty acids. This study is the first to show that healthy and YBD-compromised tissues have different infrared absorption profiles, suggesting that alterations in the biochemical composition occur during pathogenesis. Future studies should focus on determining the actual concentration of these compounds in H, AH, TR and YBD tissues and on testing the role of translocation of photoassimilates from H tissues and/or from endolithic algae to YBD tissues.
Assuntos
Antozoários/microbiologia , Espectroscopia de Infravermelho com Transformada de Fourier , Animais , Região do Caribe , Ecossistema , VibrioRESUMO
Con el propósito de valorar el efecto antipirético de un extracto fluido de Opuntia aff. soehrensii Britton & Rose se hizo esta investigación. El modelo biológico usado fue la rata Wistar. Las dosis probadas: 100, 200 y 400 mg/kg., y el control positivo utilizado fue: Antalgina 25 mg/kg. Todos los grupos constaban de 6 animales distribuidos de forma aleatoria. La variable utilizada fue la Temperatura Rectal a las 0, 4, 5, y 6 horas post- Tratamiento, el resultado demostró un efecto antipirético con 400 mg/kg, dosis máxima experimentada. La fiebre se indujo con solución al 15 por ciento de levadura desecada en cloruro de sodio al 0,9 por ciento. El nivel de significación se fijó en una probabilidad menor o igual que 0,05. Se discuten los resultados a la luz de los conocimientos actuales sobre los efectos farmacológicos atribuidos científicamente a esta planta.
