Bridging the gap between ecological succession of fleshy-fruited shrubs and restoration frameworks in semiarid oldfields.

Abandoned agricultural areas (i.e. oldfields) represent an opportunity for natural vegetation recovery, increasing soil carbon sequestration and lessening the impacts of climate change and desertification. Ecological succession in oldfields can be hampered by the harsh conditions of semiarid and arid ecosystems, and hence, restoration actions may be needed in some contexts to reactivate the ecosystem functioning. Fleshy-fruited shrubs are indicators of progression in the ecological succession, which can shift notably across environmental gradients, making difficult to obtain robust conclusions at regional scales. Other poorly studied aspects at such scales (agricultural legacy, structural features and local landscape effects) add to this knowledge gap. Here, we study the species-specific natural colonization patterns of fleshy-fruited shrubs in semiarid oldfields across environmental gradients in the Southeast of the Iberian Peninsula taking into account specific traits of plants. We used Hierarchical Modelling of Species Communities (HMSC) to test the influence of the time since the abandonment and the past land-use history of the oldfields, and the effect of local structural factors, such as the presence of remnant trees and natural patches of vegetation, on the shrub recolonization patterns. We found that altitude and lithology conditioned the structure of shrub communities, allowing the selection of different focal species for making recommendations for restoration. Time since abandonment was not relevant for the colonization process. The persistence of remnant trees in the oldfields showed a positive effect on the occurrence of several shrub species. Close sources of propagules (terrace edges and/or natural vegetation patches) benefited the occurrence of certain species mainly at lower altitudes. Traits of species (growth form, root depth, dispersal mode, fruit length and water content) helped to explain the performance of species along the environmental gradients. We identified the main drivers of natural colonization of fleshy-fruited shrubs in semiarid oldfields across environmental gradients, providing ecological knowledge to guide scientists and practitioners to develop nature-based restoration frameworks. Different management actions are recommended according to the environmental gradient.

Interdisciplinary Spanish consensus on a watch-and-wait approach for rectal cancer.

Watch-and-wait has emerged as a new strategy for the management of rectal cancer when a complete clinical response is achieved after neoadjuvant therapy. In an attempt to standardize this new clinical approach, initiated by the Spanish Cooperative Group for the Treatment of Digestive Tumors (TTD), and with the participation of the Spanish Association of Coloproctology (AECP), the Spanish Society of Pathology (SEAP), the Spanish Society of Gastrointestinal Endoscopy (SEED), the Spanish Society of Radiation Oncology (SEOR), and the Spanish Society of Medical Radiology (SERAM), we present herein a consensus on a watch-and-wait approach for the management of rectal cancer. We have focused on patient selection, the treatment schemes evaluated, the optimal timing for evaluating the clinical complete response, the oncologic outcomes after the implementation of this strategy, and a protocol for surveillance of these patients.

Depresión, ansiedad y estrés durante la pandemia COVID-19 en estudiantes de Odontología / Depression, anxiety and stress during the COVID-19 pandemic in dental students

Resumen Introducción: en diciembre del 2019, se reporta en el mundo un caso de neumonía causada por el coronavirus 2 (SARS-CoV-2). En Paraguay, actualmente se registraron más de 428.000 casos confirmados y más de 13.500 muertes. En ese contexto, la enseñanza ha tenido que convocarse al entorno virtual generando, en todos los niveles, modificaciones reactivas conductuales nacidas desde diversas causas: la dificultad al acceso a elementos informáticos, necesidad en diversos grados de reaprendizaje en un entorno no habitual y sobre todo la incertidumbre. El objetivo del estudio fue determinar los niveles de depresión, ansiedad y estrés durante la pandemia covid-19 en estudiantes de Odontología. Métodos: para este estudio observacional descriptivo de corte transversal se incluyeron estudiantes de grado de la Facultad de Odontología de la Universidad Nacional de Asunción, matriculados al periodo académico 2021. Se utilizó la escala de Depression, Anxiety and Stress (DASS-21) short form. Cada uno de los tres ejes (depresión, ansiedad y estrés) contiene 7 ítems, divididas en subescalas de contenido similar. Las puntuaciones fueron categorizadas en cinco grupos ordenados (normal, leve, moderada, severa y muy severa). Resultados: los estudiantes de Odontología presentaron cuadros de depresión y ansiedad, con síntomas de ansiedad moderada en un 42,58%; 1,29% presentó depresión severa. Conclusión: la mayoría de los estudiantes de Odontología presentaron cuadros de depresión y ansiedad con síntomas muy comunes, como la imposibilidad de relajarse, falta de iniciativa, irritabilidad y nervios.

Abstract Introduction: in December 2019, a case of pneumonia caused by coronavirus 2 (SARS-CoV-2) was reported in the world. Currently, Paraguay has registered more than 428,000 confirmed cases and more than 13,500 deaths. In this context, teaching has had to be transferred to the virtual environment, generating reactive behavioral modifications at all levels arising from various causes: difficulty in accessing computer elements, the need in varying degrees of relearning in an unusual environment and, above all, uncertainty. The aim of this study was to determine the levels of depression, anxiety and stress during the covid-19 pandemic in dental students. Methods: for this cross-sectional descriptive observational study, undergraduate students from the School of Dentistry of the National University of Asunción, enrolled in the academic period 2021, were included. The Depression, Anxiety and Stress (DASS-21) short form scale was used. Each of the three axes (depression, anxiety, and stress) contains 7 items, divided into subscales of similar content. The scores were categorized into five ordered groups (normal, mild, moderate, severe, and very severe). Results: dentistry students presented symptoms of depression and anxiety with symptoms of moderate anxiety in 42.58%; 1.29% presented severe depression . Conclusion: most of the dental students presented depression and anxiety with very common symptoms such as the inability to relax, lack of initiative, irritability and nervousness.

Muscle oxygenation of the quadriceps and gastrocnemius during maximal aerobic effort / Oxigenación muscular de cuádriceps y gastrocnemio durante el esfuerzo aeróbico máximo / Oxigenação muscular de quadríceps e gastrocnêmio durante o esforço aeróbico máximo

ABSTRACT Introduction: Near infrared spectroscopy (NIRS) is a non-invasive technique that is used in the assessment of tissue oxygenation and the monitoring of physical activity. Objective: To determine the influence of sexual, anthropometric and ergospirometric factors on muscle oxygenation of the quadriceps and gastrocnemius, obtained by NIRS during a stress test. Methods: Twenty healthy subjects participated in this study (10 women). Two Humon Hex® devices were placed on the dominant side of the quadriceps and gastrocnemius muscles to measure muscle oxygen saturation (SmO2). The stress test was performed on a treadmill with electrocardiographic control and measurement of oxygen consumption. SmO2 was obtained at rest and after maximum effort during the stress test. In addition, the height, weight, skinfold and waist contour were measured. Bioimpedance was used to obtain the percentages of fat mass and muscle mass, which were used to calculate the relative fat mass (RFM). Results: The SmO2 of both muscles at rest is higher in males than in females. At maximum effort, the SmO2 of the quadriceps is similar in both groups. The SmO2 of both muscles is positively related to height, body mass, percentage of mass muscle and waist contour, and negatively with percentage of mass fat, RFM and skinfold thickness. The negative correlation between fat percentage and oxygen saturation is more evident in females. It was observed that the variables that quantify maximum effort are not related to the SmO2 values, except for the correlation between HR max and SmO2 of the gastrocnemius muscle in males. Conclusion: The SmO2 of recreational athletes is influenced by the location of the device and the fat mass of the subjects. The biggest differences between the sexes are in the gastrocnemius muscle. Level of Evidence II; Diagnostic Studies - Investigating a Diagnostic Test .

RESUMEN Introducción: La espectroscopia de infrarrojo cercano (NIRS) es una técnica no invasiva usada en la evaluación de la oxigenación tisular y en la monitorización de la actividad física. Objetivos: Determinar la influencia de factores sexuales, antropométricos y ergoespirométricos sobre la oxigenación muscular de cuádriceps y del gastrocnemio, obtenidos por NIRS durante un test de esfuerzo. Métodos: Participaron en este estudio 20 individuos saludables (10 mujeres). Se colocaron dos dispositivos Humon Hex® en el lado dominante de los músculos cuádriceps y gastrocnemio para medir la saturación de oxígeno muscular (SmO2). El test de esfuerzo fue realizado en una cinta ergométrica con control electrocardiográfico y medición del consumo de oxígeno. La SmO2 fue obtenida en reposo y después de esfuerzo máximo durante el test. Además, fueron medidos altura, peso, pliegues cutáneos y contorno de la cintura. Fue usada bioimpedancia para obtener los porcentuales de masa grasa y masa muscular, que fueron usados para calcular la masa grasa relativa (MGR). Resultados: La SmO2 de ambos músculos en reposo es mayor en hombres que en mujeres. En el esfuerzo máximo la SmO2 del cuádriceps es semejante en los dos grupos. La SmO2 de ambos músculos es positivamente relacionada con altura, masa corporal, porcentual de masa muscular y contorno de la cintura y negativamente relacionada con el porcentaje de masa grasa, MGR y espesor de los pliegues cutáneos. La correlación negativa entre el porcentual de grasa y la saturación de oxígeno es más evidente en las mujeres. Se observó que variables que cuantifican el esfuerzo máximo no está relacionadas con los valores SmO2, excepto por la correlación entre FCmáx y SmO2 del músculo gastrocnemio en sexo masculino. Conclusión: La SmO2 de los atletas recreativos es influenciada por la localización del dispositivo y por la masa grasa de los individuos. Las mayores diferencias entre los sexos están en el músculo gastrocnemio. Nivel de Evidencia II; Estudios diagnósticos - Investigación de un examen para diagnóstico .

RESUMO Introdução: A espectroscopia de infravermelho próximo (NIRS) é uma técnica não invasiva usada na avaliação da oxigenação tecidual e no monitoramento da atividade física. Objetivos: Determinar a influência de fatores sexuais, antropométricos e ergoespirométricos sobre a oxigenação muscular do quadríceps e do gastrocnêmio obtidos por NIRS durante um teste de esforço. Métodos: Participaram deste estudo 20 indivíduos saudáveis (10 mulheres). Dois dispositivos Humon Hex® foram colocados no lado dominante dos músculos quadríceps e gastrocnêmio para medir a saturação de oxigênio muscular (SmO2). O teste de esforço foi realizado em uma esteira com controle eletrocardiográfico e mensuração do consumo de oxigênio. A SmO2foi obtida em repouso e depois de esforço máximo durante o teste. Além disso, foram medidos estatura, peso, dobras cutâneas e contorno da cintura. A bioimpedância foi usada para obter os percentuais de massa gorda e massa muscular, que foram usados para calcular a massa gorda relativa (MGR). Resultados: A SmO2de ambos os músculos em repouso é maior em homens do que em mulheres. No esforço máximo, a SmO2do quadríceps é semelhante nos dois os grupos. A SmO2de ambos os músculos é positivamente relacionada com estatura, massa corporal, percentual de massa muscular e contorno da cintura e negativamente relacionada com a porcentagem de massa gorda, MGR e espessura das dobras cutâneas. A correlação negativa entre o percentual de gordura e a saturação de oxigênio é mais evidente nas mulheres. Observou-se que variáveis que quantificam o esforço máximo não estão relacionadas com os valores SmO2, exceto pela correlação entre FCmáx e SmO2do músculo gastrocnêmio no sexo masculino. Conclusão: A SmO2dos atletas recreativos é influenciada pela localização do dispositivo e pela massa gorda dos indivíduos. As maiores diferenças entre os sexos estão no músculo gastrocnêmio. Nível de Evidência II; Estudos diagnósticos - Investigação de um exame para diagnóstico .

Prenatal Omega-6:Omega-3 Ratio and Attention Deficit and Hyperactivity Disorder Symptoms.

OBJECTIVE: To evaluate whether higher omega-6:omega-3 (n-6:n-3) long-chain polyunsaturated fatty acid ratio in cord plasma is associated with more symptoms of attention deficit and hyperactivity disorder (ADHD) at 4 and 7 years of age. STUDY DESIGN: This study was based on a population-based birth cohort in Spain. N-6 arachidonic acid and n-3 eicosapentaenoic and docosahexaenoic acid concentrations were measured in cord plasma. At 4 years old, ADHD symptoms were reported by teachers through the ADHD Diagnostic and Statistical Manual of Mental Disorders, 4th ed checklist (n = 580). At 7 years old, ADHD symptoms were reported by parents through the Conners' Rating Scale-Revised (short form; n = 642). The ADHD variable was treated as continuous (score) and as dichotomous (symptom diagnostic criteria). Child and family general characteristics were prospectively collected through questionnaires. We applied pooled zero-inflated negative binomial and logistic regressions adjusted for covariates. RESULTS: A higher omega-6:omega-3 long-chain polyunsaturated fatty acid ratio in cord plasma was associated with a higher ADHD index (incidence rate ratio, 1.13; 95% CI, 1.03, 1.23) at 7 years old. The association was not observed at 4 years old (incidence rate ratio, 1.04; 95% CI, 0.92-1.18). No associations were found using ADHD symptom diagnostic criteria. CONCLUSIONS: High prenatal omega-6:omega-3 long-chain polyunsaturated fatty acid ratio preceded the appearance of subclinical ADHD symptoms during mid-childhood. Our findings suggest that maternal diet during pregnancy may modulate the risk to develop long-term ADHD symptoms in the offspring.

Are Early Physical Activity and Sedentary Behaviors Related to Working Memory at 7 and 14 Years of Age?

OBJECTIVE: To evaluate the role of extracurricular physical activity and sedentary behavior at preschool and primary school age on working memory at primary school age and adolescence, respectively. STUDY DESIGN: This prospective study was based on a birth cohort across 4 Spanish regions. In the 3 younger subcohorts (n = 1093), parents reported lifestyle habits of child at age 4 years of age on a questionnaire, and children performed a computerized working memory task at 7 years of age. In the older subcohort (n = 307), the questionnaire was completed at 6 years of age and working memory was tested at 14 years of age. Adjusted regression models were developed to investigate the associations between lifestyle habits and working memory. RESULTS: Low extracurricular physical activity levels at 4 years of age were associated with a nonsignificant 0.95% (95% CI -2.81 to 0.92) reduction of correct responses in the working memory task at age 7 years of age. Low extracurricular physical activity levels at 6 years of age were associated with a 4.22% (95% CI -8.05 to -0.39) reduction of correct responses at age 14 years. Television watching was not associated with working memory. Other sedentary behaviors at 6 year of age were associated with a 5.07% (95% CI -9.68 to -0.46) reduction of correct responses in boys at 14 years of age. CONCLUSION: Low extracurricular physical activity levels at preschool and primary school ages were associated with poorer working memory performance at primary school age and adolescence, respectively. High sedentary behavior levels at primary school age were related negatively to working memory in adolescent boys.

[Health professionals facing hand hygiene improvement: state-of-the-art strategies versus extended strategies]. / Profesionales de salud ante la mejora de la higiene de las manos: estrategias clásicas versus estrategias avanzadas.

BACKGROUND: The hand hygiene (HH) is one of the preventive practices more .widely and effectively implemented in the control of healthcare associated infections. However, there are several barriers to compliance. OBJECTIVES: To assess which strategy, state-of-the-art strategies (availability of alcohol-based preparations, posters, instructions and training) or extended strategies (feedbacks, formal and informal leadership), are seen as more effective to improve hand hygiene (HH) compliance. METHODS: Analytical study using a self-completed questionnaire developed by the World Health Organization. 2,068 questionnaires, completed by healthcare professionals (HP) in Andalusia (Spain), were received from 2010 to 2012. Analytical technique: Structural equation modeling and multi group measurement invariance. RESULTS: Once the reliability of the proposed constructs was achieved (Cronbach α=0.73, 0.84, 0.70), it was found that those HP working in centers with the highest level of commitment with HH are those who see extended strategies as more effective (χ2=298.3, df=39, CFI=0.972, TLI=0.961, RMSEA=0.057, SRMR=0.028). DISCUSSION: Our results have shown that hospitals' HP, compared to primary care HP, see state-of-the-art strategies as more effective, as well as they give less importance to HH, meanwhile nurses, compared to physicians, see effective both strategies. HP contemplate the combination of state-of-the-art and extended strategies as an effective way to improve the HH compliance. In addition, extended strategies are considered more effective amongst the most "advanced" healthcare settings in terms of their commitment to HH. CONCLUSIONS: The results highlight the need for commitment at management, collective and individual level in order to maintain patient safety.

Profesionales de salud ante la mejora de la higiene de las manos: estrategias clásicas versus estrategias avanzadas / Health professionals facing hand hygiene improvement: state-of-the-art strategies versus extended strategies

Background: The hand hygiene (HH) is one of the preventive practices more .widely and effectively implemented in the control of healthcare associated infections. However, there are several barriers to compliance. Objectives: To assess which strategy, state-of-the-art strategies (availability of alcohol-based preparations, posters, instructions and training) or extended strategies (feedbacks, formal and informal leadership), are seen as more effective to improve hand hygiene (HH) compliance. Methods: Analytical study using a self-completed questionnaire developed by the World Health Organization. 2,068 questionnaires, completed by healthcare professionals (HP) in Andalusia (Spain), were received from 2010 to 2012. Analytical technique: Structural equation modeling and multi group measurement invariance. Results: Once the reliability of the proposed constructs was achieved (Cronbach α = 0.73, 0.84, 0.70), it was found that those HP working in centers with the highest level of commitment with HH are those who see extended strategies as more effective (χ2 = 298.3, df = 39, CFI = 0.972, TLI = 0.961, RMSEA = 0.057, SRMR = 0.028). Discussion: Our results have shown that hospitals' HP, compared to primary care HP, see state-of-the-art strategies as more effective, as well as they give less importance to HH, meanwhile nurses, compared to physicians, see effective both strategies. HP contemplate the combination of state-of-the-art and extended strategies as an effective way to improve the HH compliance. In addition, extended strategies are considered more effective amongst the most "advanced" healthcare settings in terms of their commitment to HH. Conclusions: The results highlight the need for commitment at management, collective and individual level in order to maintain patient safety.

Introducción: La higiene de las manos (HM) es una de las prácticas preventivas de mayor difusión y eficacia en el control de las infecciones asociadas a la atención en salud. Sin embargo, existen diversas barreras para su cumplimiento. Objetivos: Evaluar si son las estrategias clásicas (disponibilidad de preparados de base alcohólica, carteles, instrucciones y formación) de mejora de la HM o las estrategias avanzadas (feedbacks de cumplimiento, liderazgo formal e informal) las que se conciben como más eficaces entre los centros más involucrados con la HM. Métodos: Estudio analítico usando el cuestionario auto-cumplimentado desarrollado por la Organización Mundial de la Salud. Se recibieron 2.068 cuestionarios completos entre 2010-2012 por parte de profesionales sanitarios (PS) en Andalucía (España). Técnica analítica: modelos de ecuaciones estructurales y análisis de invarianza factorial. Resultados: Una vez se verificó la fiabilidad de los constructos propuestos (α de Cronbach: 0,73; 0,84; 0,70), se comprobó cómo aquellos PS que trabajaban en los centros más comprometidos con la HM, son aquellos que ven las estrategias avanzadas como más eficaces (χ2: 298,3; gl: 39; CFI: 0,972; TLI: 0,961; RMSEA: 0,057; SRMR: 0,028). Discusión: Se ha probado cómo en el ámbito hospitalario se ven más eficaces las estrategias clásicas y se le da menos importancia a la HM y cómo las enfermeras confían más que los médicos en todas las estrategias de mejora de la HM. Los PS contemplan la combinación de estrategias clásicas y avanzadas como el método más eficaz para la mejora de la HM. Sin embargo, las estrategias avanzadas se consideran más eficaces en aquellos centros más "maduros" en cuanto a su compromiso con la HM. Conclusiones: Los resultados ponen de manifiesto la necesidad del compromiso directivo, colectivo e individual para la seguridad del paciente.

Anatomía de la vía aérea para el broncoscopista. Una aproximación a la anestesia / Airway anatomy for the bronchoscopist: An anesthesia approach

Introducción: El conocimiento y desarrollo de habilidades en el manejo de la vía aérea es una de las competencias importantes en la formación del anestesiólogo; el «saber¼ y el «saber hacer bien y rápido¼ son determinantes en algunas situaciones críticas a las que se puede enfrentar durante el manejo anestésico. La broncoscopia es un procedimiento útil tanto diagnóstico como terapéutico. El conocimiento de la técnica y de la anatomía de la vía aérea constituye el pilar de la broncoscopia, encontrando diferentes variaciones anatómicas y clasificaciones de la vía aérea. Objetivo: Describir la anatomía de la vía aérea a través de esquemas, evaluar variaciones anatómicas y conocer características propias del procedimiento. Metodología: Con las palabras clave «Bronchoscopy¼, «Anatomy¼, «Airway¼ y «Anesthesia¼ se realizó una revisión no sistemática en bases de datos (PUBMED/MEDLINE, Science Direct, OVID, SciELO). Resultados y conclusiones: La broncoscopia es un procedimiento útil en el plano quirúrgico y diagnóstico, siendo utilizado en distintos procedimientos. Las variaciones anatómicas de la vía aérea se presentan en un porcentaje pequeño de la población. Las clasificaciones anatómicas son diversas tanto anatómica como numéricamente; sin embargo, lo relevante es desarrollar una relación espacial. La broncoscopia es una técnica que va en desarrollo paralelo a otros avances de la tecnología biomédica, es un procedimiento del cual el anestesiólogo debe investigar más con el fin de generar mejores efectos en el campo de la anestesiología.

Introduction: Knowledge and development of skills in the management of the airway is one of important competencies in the training of the anesthesiologist, "knowledge" and "know how well and fast" are decisive in some critical situations during the anesthetic management. Bronchoscopy is a useful both diagnostic and therapeutic procedure. Knowledge of technique and the anatomy of the airway is the key of bronchoscopy, finding different anatomic variations and classifications of the airway. Objective: Describe the airway anatomy through diagrams, evaluate anatomic variations and characteristics of procedure. Methodology: With the keywords "Bronchoscopy" and "Anatomy", "Airway", "Anesthesia" held a non-systematic review databases (PUBMED/MEDLINE, OVID, Science Direct, SciELO). Results and conclusions: The bronchoscopy is a useful procedure in the surgical level and diagnosis, being used in various procedures. Airway anatomical variations occur in a small percentage of the population. Anatomical classifications are different both anatomic as numerically, but what is important is developing a spatial relation. Bronchoscopy is a technique that goes in parallel development of other advances in biomedical technology, is a procedure whereby the anesthesiologist should be investigated in order to generate better effects in the field of the anesthesiology.

Generación de preguntas sobre información no textual: una validación empírica del modelo obstáculo-meta en la comprensión de dispositivos experimentales de ciencias / Questions Generated on Non-Textual Information: An Empirical Validation of the Obstacle-Goal Model Applied to the Comprehension of Scientific Experimental Devices

Los mecanismos cognitivos de la generación de preguntas no son aún bien conocidos. Recientemente, se ha propuesto el modelo obstáculo-meta que asimila la comprensión a un proceso de resolución de un problema cognitivo, cuyos obstáculos hacia la meta pretendida dan lugar a las preguntas. Una predicción del modelo es la relación entre tipos de preguntas formuladas y tipos de representación mental que los sujetos manejan durante la comprensión de la información suministrada. Hasta ahora, esta predicción ha sido validada parcialmente y solo con información textual. En este trabajo se presenta un estudio empírico de validación más fiable de esta predicción, desarrollado en dos fases y que utiliza información no textual, como es el funcionamiento de dispositivos experimentales. Los resultados apoyan la predicción del modelo con suficiente potencia estadística.

The cognitive mechanisms underlying question generation are not yet well understood. Recently, the Obstacle-Goal model has been proposed. This model assimilates comprehension to a problem-solving cognitive process: the obstacles in the way to the intended goal originate the questions asked. A direct prediction from this model is the relationship between the distribution of types of questions asked, and the kinds of mental representations subjects elaborate to understand the provided information. Up to now, this prediction has been partially validated, and only textual information has been used to this purpose. In the present paper an empirical two-phase study is developed to validate the above prediction in a more reliable way, using non-textual information: the operation of experimental devices. Results support the prediction of the model with enough statistical power.

Single or combined effects of Lactobacillus sakei and inulin on growth, non-specific immunity and IgM expression in leopard grouper (Mycteroperca rosacea).

The aim of this study was to evaluate the single or combined effects of Lactobacillus sakei with inulin suitable for immunological in vivo studies in farmed fish. By in vitro assays, L. sakei strain 5-4 showed antibacterial activities against all assayed fish pathogens (except the Vibrio harveyi strain CAIM-1793). L. sakei was able to survive at high fish bile concentrations. Fermentation of the agave inulin resulted in a large increase in number of lactobacilli. For the in vivo study, fish were fed for 8 weeks four practical diets: control diet (control), L. sakei 5-4 (10(7) CFU/g), inulin (1% or 10 g/kg) and L. sakei + inulin (10(7) CFU/g + 10 g/kg). The weight gain showed clearly the synergistic effect of L. sakei 5-4 and inulin at 6 and 8 weeks of treatments. Leopard grouper fed with L. sakei alone or combined with inulin have significantly increased the assayed physiological and humoral immune parameters. By real-time PCR assays, the mRNA transcripts of immunoglobulin M (IgM) were found to be higher expressed in intestine, head kidney, mucus, gill, spleen and skin. Moreover, mRNA expression levels of IgM in head kidney and anterior intestine were measured by real-time PCR. L. sakei 5-4 and L. sakei + inulin supplemented diet up-regulated the expression of IgM at week 4 and 8 in intestine and head kidney, respectively. These results support the idea that the L. sakei 5-4 alone or combined with agave inulin improved growth performance and stimulates the immune system of leopard grouper.

[Pancreatic pseudocyst infection caused by Haemophilus parainfluenzae. Report of one case]. / Infección de pseudoquiste pancreático por Haemophilus parainfluenzae. Caso clínico.

We report a 48-year-old male admitted to hospital due to a severe alcoholic pancreatitis. At four weeks of evolution of the acute episode, an abdominal CAT scan showed a fluid collection of 20 cm diameter located in the pancreatic tail and 2 small collections in the head. The patient received several antimicrobials and during the seventh week of evolution, while receiving vancomycin, presented fever. A fine needle aspiration of the cyst revealed the presence of Haemophilus parainfluenzae biotype VIII. The patient was treated with amoxicillin-clavulanic acid and a laparoscopic cysto-gastrostomy, with a good clinical response.

Modeling the pulsed light inactivation of microorganisms naturally occurring on vegetable substrates.

Pulsed light (PL) is a fast non-thermal method for microbial inactivation. This research studied the kinetics of PL inactivation of microorganisms naturally occurring in some vegetables. Iceberg lettuce, white cabbage and Julienne-style cut carrots were subjected to increasing PL fluences up to 12J/cm(2) in order to study its effect on aerobic mesophilic bacteria determined by plate count. Also, sample temperature increase was determined by infrared thermometry. Survivors' curves were adjusted to several models. No shoulder but tail was observed. The Weibull model showed good fitting performance of data. Results for lettuce were: goodness-of-fit parameter RMSE=0.2289, fluence for the first decimal reduction δ=0.98±0.80J/cm(2) and concavity parameter p=0.33±0.08. Results for cabbage were: RMSE=0.0725, δ=0.81±0.23J/cm(2) and p=0.30±0.02; and for carrot: RMSE=0.1235, δ=0.39±0.24J/cm(2) and p=0.23±0.03. For lettuce, a log-linear and tail model was also suitable. Validation of the Weibull model produced determination coefficients of 0.88-0.96 and slopes of 0.78-0.99. Heating was too low to contribute to inactivation. A single low-energy pulse was enough to achieve one log reduction, with an ultrafast treatment time of 0.5ms. While PL efficacy was found to be limited to high residual counts, the achievable inactivation level may be considered useful for shelf-life extension.

Infección de pseudoquiste pancreático por Haemophilus parainfluenzae: Caso clínico / Pancreatic pseudocyst infection caused by Haemophilus parainfluenzae: Report of one case

We report a 48-year-old male admitted to hospital due to a severe alcoholic pancreatitis. At four weeks of evolution of the acute episode, an abdominal CAT scan showed a fluid collection of 20 cm diameter located in the pancreatic tail and 2 small collections in the head. The patient received several antimicrobials and during the seventh week of evolution, while receiving vancomycin, presented fever. A fine needle aspiration of the cyst revealed the presence of Haemophilus parainfluenzae biotype VIII. The patient was treated with amoxicillin-clavulanic acid and a laparoscopic cysto-gastrostomy, with a good clinical response.

Evaluación in vitro de la filtración coronaria a través de dos técnicas de obturación de gutapercha plastificada: híbrida de tagger y guttaflow / In vitro evaluation of coronary filtration through two sealing techniques plasticized gutta percha: Hybrid tagger and GuttaFlow

El objetivo de este trabajo fue evaluar la filtración vía coronaria de dos técnicas de obturación diferentes: la técnica Híbrida de Tagger y el Sistema Guttaflow. Cincuenta primeros premolares inferiores humanos fueron preparados con la técnica crown-dowm y divididos aleatoriamente en dos grupos de 20 dientes. Otros 5 dientes fueron utilizados como grupo control negativo y otros 5 dientes como grupo control positivo. El grupo 1 fue obturado con la técnica Hibrida de Tagger con Sealer 26 como cemento obturador y el grupo 2 fue obturado con el sistema Guttaflow. Los dientes fueron restaurados de forma provisional con Coltosol y colocados en una estufa a 37° C con una humedad de 95%. Después de permanecer 30 días en la estufa, fue retirada la restauración provisional y los dientes fueron dejados en la estufa por 30 días más. Fue realizada la impermeabilización con cianoacrilato de etilo. Los dientes fueron inmersos en rodamina b al 2% durante 72 horas. Cada diente fue cortado de mesial a distal para observar el grado de filtración. Los dientes fueron escaneados y medidos mediante el software Image Tools Image Tools v.3.00. Las dos técnicas de obturación permitieron una filtración del colorante vía coronaria; pero entre los dos grupos se observó una diferencia estadística importante. En el grupo 1 (Hibrida de Tagger) se observó una filtración promedio de 0,96 mm y en el grupo 2 (Guttaflow) se observó una infiltración promedio de 2,36 mm. Los resultados mostraron que la técnica Híbrida de Tagger ofreció un mejor sellado de los conductos en comparación al sistema GuttaFlow

The purpose of this research was to evaluate the coronary's infiltration of two different filling techniques, the Tagger´s Hybrid technique and the Guttaflow System. Fifty inferior premolar human teeth were prepared using the crown-down technique, and then divided into two groups of twenty each. Five extra teeth were used as a positive control group, and other five as a negative control group. The first group was filled with the Tagger´s Hybrid technique using Sealer 26 as a root filler compound. On the other hand, Guttaflow System was used on the second group. All of the teeth were temporary restaured with Coltosol and placed in a stove at 37º C and 95 % humidity. After 30 days in the stove, the temporary filling was removed and remained for another 30 days. The impermeabilization was made with ethil of cyanoacrylate . All the teeth were immersed in 2% Rodamine b for 72 hours. Each tooth was cut from mesial to distal side to observe the infiltration amount. The teeth were scaned and measure by Image Tools v.3.00 software. The two filling techniques allowed colorant infiltration throw the crown; but was observed an important statistic difference. The first group (Tagger´s Hybrid technique) showed an average infiltration of 0.96mm and the second group (Guttaflow) 2.36mm. The results of this research showed that the Tagger´s Hybrid technique presented less coronary's infiltration than the Guttaflow

El Triage de enfermería: la consulta de admisión, recepción y clasificación / The nursing triag: the admission, reception and classification visit

Los autores describen el método del Triage para priorizar y optimizar el uso de los recursos, principalmente el factor tiempo y humano, a fin de que aquellos pacientes que realmente necesitan una atención rápida, la obtengan, contribuyendo además a una atención de mayor calidad (AU)

El Triage de enfermería: la consulta de admisión, recepción y clasificación / The nursing triag: the admission, reception and classification visit

Los autores describen el método del Triage para priorizar y optimizar el uso de los recursos, principalmente el factor tiempo y humano, a fin de que aquellos pacientes que realmente necesitan una atención rápida, la obtengan, contribuyendo además a una atención de mayor calidad

Fruit characterization of high oil content avocado varieties

To expand the data bank on avocado (Persea americana Mill.) varieties all over the world, and to select good varieties for commercial or improving purposes, a partial fruit characterization of 13 high oil content (11.23-18.80%) was performed. The chosen varieties are growing in a Venezuelan germplasm bank: Fuerte, Peruano, Lula, Ortega, Red Collinson, Alcemio, Araira 1, Pope, Ettinger, Gripiña 5, Barker, Duke, and Ryan. They were characterized for pulp oil and moisture; weight (whole fruit, seed, pulp and peel); length, width and fruit shape; peel characteristics (roughness, color and hand peeling); and ripeness time. The variety Ryan showed the highest oil content (18.80%) and calorific value (191 Kcal/100 g wet flesh). Avocado varieties grown in Venezuela have generally less oil content and are generally lighter than those from other countries. Most of the varieties had low pulp proportion, and were pyriform, with rough green peel and difficult to hand peel. Red Collinson had an uncommon reddish peel. The ripening time was between 4 and 10 days after harvest.

O teor de óleo de abacates (Persea americana Mill.)é uma característica importante para seu consumo in natura e para a indústria. Treze variedades de abacate de alto teor de óleo (11,23-18,80%) de um pomar venezuelano: Fuerte, Peruano, Lula Ortega, Red Collison, Alcemio, Araira 1, Pope, Ettinger, Gripiña 5, Barker, Duke e Ryan, foram caracterizados pelo teor de óleo da polpa e umidade; peso (fruto inteiro, semente, polpa e casca); comprimento, largura e forma; características da casca (rugosidade, cor e soltura); e tempo de maturação. A variedade Ryan mostrou os maiores teor de óleo (18,80%) e valor calórico (191 kcal/100 g de peso fresco). As variedades venezuelanas têm, em geral, teor mais baixo de gordura e proporção de polpa, e são em geral mais leves que aquelas de outros países. A maioria das variedades é periforme, com casca verde áspera e difíceis de serem descascadas manualmente. A variedade Red Collison tem uma cor avermelhada incomum. A maturação esteve entre 4 e 10 dias após a colheita.